A call for caution in analysing mammalian co-transfection experiments and implications of resource competition in data misinterpretation

Transient transfections are routinely used in basic and synthetic biology studies to unravel pathway regulation and to probe and characterise circuit designs. As each experiment has a component of intrinsic variability, reporter gene expression is usually normalized with co-delivered genes that act...

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Autores principales: Roberto Di Blasi, Masue M. Marbiah, Velia Siciliano, Karen Polizzi, Francesca Ceroni
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/8edf6ae5ac17436485b407b1d9cf9f05
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