Electrospun Scaffold with Sustained Antibacterial and Tissue-Matched Mechanical Properties for Potential Application as Functional Mesh

Zhengni Liu, Xiaoqiang Zhu, Rui Tang Department of Hernia and Abdominal Wall Surgery, Shanghai East Hospital, Tongji University, Shanghai, People’s Republic of ChinaCorrespondence: Rui TangDepartment of Hernia and Abdominal Wall Surgery, Shanghai East Hospital, Tongji University, Shanghai,...

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Autores principales:	Liu Z, Zhu X, Tang R
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	functional mesh antibacterial activity sustained release tissue-matched mechanical properties electrospinning Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/8ee9f977c2144265abbab09c2b4f2726
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spelling	oai:doaj.org-article:8ee9f977c2144265abbab09c2b4f27262021-12-02T15:31:13ZElectrospun Scaffold with Sustained Antibacterial and Tissue-Matched Mechanical Properties for Potential Application as Functional Mesh1178-2013https://doaj.org/article/8ee9f977c2144265abbab09c2b4f27262020-07-01T00:00:00Zhttps://www.dovepress.com/electrospun-scaffold-with-sustained-antibacterial-and-tissue-matched-m-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Zhengni Liu, Xiaoqiang Zhu, Rui Tang Department of Hernia and Abdominal Wall Surgery, Shanghai East Hospital, Tongji University, Shanghai, People’s Republic of ChinaCorrespondence: Rui TangDepartment of Hernia and Abdominal Wall Surgery, Shanghai East Hospital, Tongji University, Shanghai, People’s Republic of ChinaEmail kevintown1972@126.comIntroduction: Various materials and approaches have been used to reduce the mesh-induced inflammatory response and modify the mesh with tissue-matched mechanical properties, aiming to improve the repair of abdominal wall defects.Materials and Methods: In this study, we fabricated a polycaprolactone (PCL)/silk fibroin (SF) mesh integrated with amoxicillin (AMX)-incorporating multiwalled carbon nanotubes (MWCNTs) via electrospinning, grafting and crosslinking, developing a sustainable antibiotic and flexible mesh. AMX was loaded into the hollow tubular MWCNTs by physical adsorption, and a nanofibrous structure was constructed by electrospinning PCL and SF (40:60 w/w). The AMX@MWCNTs were then chemically grafted onto the surfaces of the PCL/SF nanofibers by treating with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) solution for simultaneous crosslinking and coating. The incorporation of AMX into the MWCNTs (AMX@MWCNTs) and the integration of the AMX@MWCNTs with the PCL/SF nanofibers were characterized. Then, the functional mesh was fabricated and fully evaluated in terms of antibacterial activity, mechanical properties and host response.Results: Our results demonstrated that the PCL/SF nanofibrous structure was fabricated successfully by electrospinning. After integrating with AMX@MWCNT by grafting and crosslinking, the functional mesh showed undeformed structure, modified surface hydrophilicity and biocompatible interfaces, abdominal wall-matched mechanical properties, and a sustained-release antibiotic profile in E. coli growth inhibition compared to those of PCL/SF mesh in vitro. In a rat model with subcutaneous implantation, the functional mesh incited less mesh-induced inflammatory and foreign body responses than PCL/SF mesh within 14 days. The histological analysis revealed less infiltration of granulocytes and macrophages during this period, resulting in the loosely packed collagen deposition on the functional mesh and prominent collagen incorporation.Discussion: Therefore, this designed PCL/SF–AMX@MWCNT nanofibrous mesh, functionalized with antibacterial and tissue-matched mechanical properties, provides a promising alternative for the repair of abdominal wall defects.Keywords: functional mesh, antibacterial activity, sustained release, tissue-matched mechanical properties, electrospinningLiu ZZhu XTang RDove Medical Pressarticlefunctional meshantibacterial activitysustained releasetissue-matched mechanical propertieselectrospinningMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 4991-5004 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	functional mesh antibacterial activity sustained release tissue-matched mechanical properties electrospinning Medicine (General) R5-920
spellingShingle	functional mesh antibacterial activity sustained release tissue-matched mechanical properties electrospinning Medicine (General) R5-920 Liu Z Zhu X Tang R Electrospun Scaffold with Sustained Antibacterial and Tissue-Matched Mechanical Properties for Potential Application as Functional Mesh
description	Zhengni Liu, Xiaoqiang Zhu, Rui Tang Department of Hernia and Abdominal Wall Surgery, Shanghai East Hospital, Tongji University, Shanghai, People’s Republic of ChinaCorrespondence: Rui TangDepartment of Hernia and Abdominal Wall Surgery, Shanghai East Hospital, Tongji University, Shanghai, People’s Republic of ChinaEmail kevintown1972@126.comIntroduction: Various materials and approaches have been used to reduce the mesh-induced inflammatory response and modify the mesh with tissue-matched mechanical properties, aiming to improve the repair of abdominal wall defects.Materials and Methods: In this study, we fabricated a polycaprolactone (PCL)/silk fibroin (SF) mesh integrated with amoxicillin (AMX)-incorporating multiwalled carbon nanotubes (MWCNTs) via electrospinning, grafting and crosslinking, developing a sustainable antibiotic and flexible mesh. AMX was loaded into the hollow tubular MWCNTs by physical adsorption, and a nanofibrous structure was constructed by electrospinning PCL and SF (40:60 w/w). The AMX@MWCNTs were then chemically grafted onto the surfaces of the PCL/SF nanofibers by treating with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) solution for simultaneous crosslinking and coating. The incorporation of AMX into the MWCNTs (AMX@MWCNTs) and the integration of the AMX@MWCNTs with the PCL/SF nanofibers were characterized. Then, the functional mesh was fabricated and fully evaluated in terms of antibacterial activity, mechanical properties and host response.Results: Our results demonstrated that the PCL/SF nanofibrous structure was fabricated successfully by electrospinning. After integrating with AMX@MWCNT by grafting and crosslinking, the functional mesh showed undeformed structure, modified surface hydrophilicity and biocompatible interfaces, abdominal wall-matched mechanical properties, and a sustained-release antibiotic profile in E. coli growth inhibition compared to those of PCL/SF mesh in vitro. In a rat model with subcutaneous implantation, the functional mesh incited less mesh-induced inflammatory and foreign body responses than PCL/SF mesh within 14 days. The histological analysis revealed less infiltration of granulocytes and macrophages during this period, resulting in the loosely packed collagen deposition on the functional mesh and prominent collagen incorporation.Discussion: Therefore, this designed PCL/SF–AMX@MWCNT nanofibrous mesh, functionalized with antibacterial and tissue-matched mechanical properties, provides a promising alternative for the repair of abdominal wall defects.Keywords: functional mesh, antibacterial activity, sustained release, tissue-matched mechanical properties, electrospinning
format	article
author	Liu Z Zhu X Tang R
author_facet	Liu Z Zhu X Tang R
author_sort	Liu Z
title	Electrospun Scaffold with Sustained Antibacterial and Tissue-Matched Mechanical Properties for Potential Application as Functional Mesh
title_short	Electrospun Scaffold with Sustained Antibacterial and Tissue-Matched Mechanical Properties for Potential Application as Functional Mesh
title_full	Electrospun Scaffold with Sustained Antibacterial and Tissue-Matched Mechanical Properties for Potential Application as Functional Mesh
title_fullStr	Electrospun Scaffold with Sustained Antibacterial and Tissue-Matched Mechanical Properties for Potential Application as Functional Mesh
title_full_unstemmed	Electrospun Scaffold with Sustained Antibacterial and Tissue-Matched Mechanical Properties for Potential Application as Functional Mesh
title_sort	electrospun scaffold with sustained antibacterial and tissue-matched mechanical properties for potential application as functional mesh
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/8ee9f977c2144265abbab09c2b4f2726
work_keys_str_mv	AT liuz electrospunscaffoldwithsustainedantibacterialandtissuematchedmechanicalpropertiesforpotentialapplicationasfunctionalmesh AT zhux electrospunscaffoldwithsustainedantibacterialandtissuematchedmechanicalpropertiesforpotentialapplicationasfunctionalmesh AT tangr electrospunscaffoldwithsustainedantibacterialandtissuematchedmechanicalpropertiesforpotentialapplicationasfunctionalmesh
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Electrospun Scaffold with Sustained Antibacterial and Tissue-Matched Mechanical Properties for Potential Application as Functional Mesh

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