Ultrasonic Nanoemulsification of Cuminum cyminum Essential Oil and Its Applications in Medicine

M Joyce Nirmala, 1 Latha Durai, 2 Kagitala Anvesh Rao, 1 R Nagarajan 1 1Department of Chemical Engineering, Indian Institute of Technology Madras, Chennai 600 036, India; 2Department of Biotechnology, Bhupat & Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, Chenna...

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Autores principales:	Nirmala MJ, Durai L, Rao KA, Nagarajan R
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	cumin oil nanoemulsion tongue carcinoma cell line apoptosis antibacterial activity anticancer activity. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/8eedd6c2cad044d386ba2622ef31c761
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spelling	oai:doaj.org-article:8eedd6c2cad044d386ba2622ef31c7612021-12-02T05:44:58ZUltrasonic Nanoemulsification of Cuminum cyminum Essential Oil and Its Applications in Medicine1178-2013https://doaj.org/article/8eedd6c2cad044d386ba2622ef31c7612020-02-01T00:00:00Zhttps://www.dovepress.com/ultrasonic-nanoemulsification-of-cuminum-cyminum-essential-oil-and-its-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013M Joyce Nirmala, 1 Latha Durai, 2 Kagitala Anvesh Rao, 1 R Nagarajan 1 1Department of Chemical Engineering, Indian Institute of Technology Madras, Chennai 600 036, India; 2Department of Biotechnology, Bhupat & Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, Chennai 600036, IndiaCorrespondence: M Joyce NirmalaDepartment of Chemical Engineering, Indian Institute of Technology Madras, Chennai 600 036, IndiaEmail joycegitz@gmail.comBackground and Study: Cumin seed oil (extracted from Cuminum cyminum) has many applications but conclusive evidence of its therapeutic uses has not been presented. This study has explored the anticancer and antibacterial properties of the seed oil.Methods: The cumin nanoemulsion was prepared with Tween 80 non-ionic surfactant employing ultra-sonication technology. The anticancer activity of the nanoscale-based emulsion was evaluated through cell viability (MTT), antiproliferation evaluation through clonogenic assay, and apoptosis through Annexin V-FITC assay. Agar well diffusion was used to study the antimicrobial activity, and this was supported by membrane integrity analysis.Results: A thorough study of process parameters, aimed at obtaining the optimal surface concentration and emulsification time, was completed. GC-MS data indicated cumaldehyde as a major component. The resultant droplet diameter after a sonication time of 5 min was 10.4 ± 0.5 nm. MTT assay revealed the IC 50 value at 1.5 μL/mL and the early induction of apoptosis was evident. Tongue carcinoma cell line treated with cumin nanoemulsion presented a diminished colony formation. The nanoemulsion exhibited significant antibacterial activity against S. aureus. A significant cytoplasmic leakage was observed on treatment with cumin nanoemulsion. The consequences of the analysis projected cumin as a potential component for cancer therapy.Conclusion: This study provides definitive evidence for cumin essential oil nanoemulsion as a legitimate plant-based medicine that can bypass the drawbacks of the present aggressive treatment of cancer, can overcome the antimicrobial resistance, and can also meet all prerequisites.Keywords: cumin oil, nanoemulsion, tongue carcinoma cell line, apoptosis, antibacterial activity, anticancer activityNirmala MJDurai LRao KANagarajan RDove Medical Pressarticlecumin oilnanoemulsiontongue carcinoma cell lineapoptosisantibacterial activityanticancer activity.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 795-807 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	cumin oil nanoemulsion tongue carcinoma cell line apoptosis antibacterial activity anticancer activity. Medicine (General) R5-920
spellingShingle	cumin oil nanoemulsion tongue carcinoma cell line apoptosis antibacterial activity anticancer activity. Medicine (General) R5-920 Nirmala MJ Durai L Rao KA Nagarajan R Ultrasonic Nanoemulsification of Cuminum cyminum Essential Oil and Its Applications in Medicine
description	M Joyce Nirmala, 1 Latha Durai, 2 Kagitala Anvesh Rao, 1 R Nagarajan 1 1Department of Chemical Engineering, Indian Institute of Technology Madras, Chennai 600 036, India; 2Department of Biotechnology, Bhupat & Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, Chennai 600036, IndiaCorrespondence: M Joyce NirmalaDepartment of Chemical Engineering, Indian Institute of Technology Madras, Chennai 600 036, IndiaEmail joycegitz@gmail.comBackground and Study: Cumin seed oil (extracted from Cuminum cyminum) has many applications but conclusive evidence of its therapeutic uses has not been presented. This study has explored the anticancer and antibacterial properties of the seed oil.Methods: The cumin nanoemulsion was prepared with Tween 80 non-ionic surfactant employing ultra-sonication technology. The anticancer activity of the nanoscale-based emulsion was evaluated through cell viability (MTT), antiproliferation evaluation through clonogenic assay, and apoptosis through Annexin V-FITC assay. Agar well diffusion was used to study the antimicrobial activity, and this was supported by membrane integrity analysis.Results: A thorough study of process parameters, aimed at obtaining the optimal surface concentration and emulsification time, was completed. GC-MS data indicated cumaldehyde as a major component. The resultant droplet diameter after a sonication time of 5 min was 10.4 ± 0.5 nm. MTT assay revealed the IC 50 value at 1.5 μL/mL and the early induction of apoptosis was evident. Tongue carcinoma cell line treated with cumin nanoemulsion presented a diminished colony formation. The nanoemulsion exhibited significant antibacterial activity against S. aureus. A significant cytoplasmic leakage was observed on treatment with cumin nanoemulsion. The consequences of the analysis projected cumin as a potential component for cancer therapy.Conclusion: This study provides definitive evidence for cumin essential oil nanoemulsion as a legitimate plant-based medicine that can bypass the drawbacks of the present aggressive treatment of cancer, can overcome the antimicrobial resistance, and can also meet all prerequisites.Keywords: cumin oil, nanoemulsion, tongue carcinoma cell line, apoptosis, antibacterial activity, anticancer activity
format	article
author	Nirmala MJ Durai L Rao KA Nagarajan R
author_facet	Nirmala MJ Durai L Rao KA Nagarajan R
author_sort	Nirmala MJ
title	Ultrasonic Nanoemulsification of Cuminum cyminum Essential Oil and Its Applications in Medicine
title_short	Ultrasonic Nanoemulsification of Cuminum cyminum Essential Oil and Its Applications in Medicine
title_full	Ultrasonic Nanoemulsification of Cuminum cyminum Essential Oil and Its Applications in Medicine
title_fullStr	Ultrasonic Nanoemulsification of Cuminum cyminum Essential Oil and Its Applications in Medicine
title_full_unstemmed	Ultrasonic Nanoemulsification of Cuminum cyminum Essential Oil and Its Applications in Medicine
title_sort	ultrasonic nanoemulsification of cuminum cyminum essential oil and its applications in medicine
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/8eedd6c2cad044d386ba2622ef31c761
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Ultrasonic Nanoemulsification of Cuminum cyminum Essential Oil and Its Applications in Medicine

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