Small RNA Profiling in Mycobacterium Provides Insights Into Stress Adaptability

Mycobacteria encounter a number of environmental changes during infection and respond using different mechanisms. Small RNA (sRNA) is a post-transcriptionally regulatory system for gene functions and has been investigated in many other bacteria. This study used Mycobacterium tuberculosis and Mycobac...

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Autores principales:	Yingyu Chen, Wenjun Zhai, Kailun Zhang, Han Liu, Tingting Zhu, Li Su, Luiz Bermudez, Huanchun Chen, Aizhen Guo
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Mycobacterium bovis Mycobacterium bovis BCG sRNA stress mycobacteria Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/8eef4f7fd8074eddbb5b486d19aa34f4
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spelling	oai:doaj.org-article:8eef4f7fd8074eddbb5b486d19aa34f42021-11-04T08:31:25ZSmall RNA Profiling in Mycobacterium Provides Insights Into Stress Adaptability1664-302X10.3389/fmicb.2021.752537https://doaj.org/article/8eef4f7fd8074eddbb5b486d19aa34f42021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.752537/fullhttps://doaj.org/toc/1664-302XMycobacteria encounter a number of environmental changes during infection and respond using different mechanisms. Small RNA (sRNA) is a post-transcriptionally regulatory system for gene functions and has been investigated in many other bacteria. This study used Mycobacterium tuberculosis and Mycobacterium bovis Bacillus Calmette-Guérin (BCG) infection models and sequenced whole bacterial RNAs before and after host cell infection. A comparison of differentially expressed sRNAs using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) and target prediction was carried out. Six pathogenically relevant stress conditions, growth rate, and morphology were used to screen and identify sRNAs. From these data, a subset of sRNAs was differentially expressed in multiple infection groups and stress conditions. Many were found associated with lipid metabolism. Among them, ncBCG427 was significantly downregulated when BCG entered into macrophages and was associated with increased biofilm formation. The reduction of virulence possibility depends on regulating lipid metabolism.Yingyu ChenYingyu ChenWenjun ZhaiWenjun ZhaiKailun ZhangKailun ZhangHan LiuHan LiuTingting ZhuTingting ZhuLi SuLi SuLuiz BermudezHuanchun ChenHuanchun ChenAizhen GuoAizhen GuoFrontiers Media S.A.articleMycobacterium bovisMycobacterium bovis BCGsRNAstressmycobacteriaMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Mycobacterium bovis Mycobacterium bovis BCG sRNA stress mycobacteria Microbiology QR1-502
spellingShingle	Mycobacterium bovis Mycobacterium bovis BCG sRNA stress mycobacteria Microbiology QR1-502 Yingyu Chen Yingyu Chen Wenjun Zhai Wenjun Zhai Kailun Zhang Kailun Zhang Han Liu Han Liu Tingting Zhu Tingting Zhu Li Su Li Su Luiz Bermudez Huanchun Chen Huanchun Chen Aizhen Guo Aizhen Guo Small RNA Profiling in Mycobacterium Provides Insights Into Stress Adaptability
description	Mycobacteria encounter a number of environmental changes during infection and respond using different mechanisms. Small RNA (sRNA) is a post-transcriptionally regulatory system for gene functions and has been investigated in many other bacteria. This study used Mycobacterium tuberculosis and Mycobacterium bovis Bacillus Calmette-Guérin (BCG) infection models and sequenced whole bacterial RNAs before and after host cell infection. A comparison of differentially expressed sRNAs using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) and target prediction was carried out. Six pathogenically relevant stress conditions, growth rate, and morphology were used to screen and identify sRNAs. From these data, a subset of sRNAs was differentially expressed in multiple infection groups and stress conditions. Many were found associated with lipid metabolism. Among them, ncBCG427 was significantly downregulated when BCG entered into macrophages and was associated with increased biofilm formation. The reduction of virulence possibility depends on regulating lipid metabolism.
format	article
author	Yingyu Chen Yingyu Chen Wenjun Zhai Wenjun Zhai Kailun Zhang Kailun Zhang Han Liu Han Liu Tingting Zhu Tingting Zhu Li Su Li Su Luiz Bermudez Huanchun Chen Huanchun Chen Aizhen Guo Aizhen Guo
author_facet	Yingyu Chen Yingyu Chen Wenjun Zhai Wenjun Zhai Kailun Zhang Kailun Zhang Han Liu Han Liu Tingting Zhu Tingting Zhu Li Su Li Su Luiz Bermudez Huanchun Chen Huanchun Chen Aizhen Guo Aizhen Guo
author_sort	Yingyu Chen
title	Small RNA Profiling in Mycobacterium Provides Insights Into Stress Adaptability
title_short	Small RNA Profiling in Mycobacterium Provides Insights Into Stress Adaptability
title_full	Small RNA Profiling in Mycobacterium Provides Insights Into Stress Adaptability
title_fullStr	Small RNA Profiling in Mycobacterium Provides Insights Into Stress Adaptability
title_full_unstemmed	Small RNA Profiling in Mycobacterium Provides Insights Into Stress Adaptability
title_sort	small rna profiling in mycobacterium provides insights into stress adaptability
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/8eef4f7fd8074eddbb5b486d19aa34f4
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Small RNA Profiling in Mycobacterium Provides Insights Into Stress Adaptability

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