Cell Culture Isolation and Whole Genome Characterization of Hepatitis E Virus Strains from Wild Boars in Germany

Infection with hepatitis E virus (HEV) can cause acute and chronic hepatitis in humans. The HEV genotype 3 can be zoonotically transmitted from animals to humans, with wild boars representing an important reservoir species. Cell culture isolation of HEV is generally difficult and mainly described fo...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Katja Schilling-Loeffler, Oliver Viera-Segura, Victor Max Corman, Julia Schneider, Ashish K. Gadicherla, Ulrich Schotte, Reimar Johne
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	hepatitis E virus wild boar cell culture hypervariable region whole genome sequencing zoonosis Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/8ef4c5a954544adf93f9f3c1acb9f598
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8ef4c5a954544adf93f9f3c1acb9f598
record_format	dspace
spelling	oai:doaj.org-article:8ef4c5a954544adf93f9f3c1acb9f5982021-11-25T18:24:57ZCell Culture Isolation and Whole Genome Characterization of Hepatitis E Virus Strains from Wild Boars in Germany10.3390/microorganisms91123022076-2607https://doaj.org/article/8ef4c5a954544adf93f9f3c1acb9f5982021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2302https://doaj.org/toc/2076-2607Infection with hepatitis E virus (HEV) can cause acute and chronic hepatitis in humans. The HEV genotype 3 can be zoonotically transmitted from animals to humans, with wild boars representing an important reservoir species. Cell culture isolation of HEV is generally difficult and mainly described for human isolates so far. Here, five sera and five liver samples from HEV-RNA-positive wild boar samples were inoculated onto PLC/PRF/5 cells, incubated for 3 months and thereafter passaged for additional 6 weeks. As demonstrated by RT-qPCR, immunofluorescence and immune electron microscopy, virus was successfully isolated from two liver samples, which originally contained high HEV genome copy numbers. Both isolates showed slower growth than the culture-adapted HEV strain 47832c. In contrast to this strain, the isolated strains had no insertions in their hypervariable genome region. Next generation sequencing using an HEV sequence-enriched library enabled full genome sequencing. Strain Wb108/17 belongs to subtype 3f and strain Wb257/17 to a tentative novel subtype recently described in Italian wild boars. The results indicate that HEV can be successfully isolated in cell culture from wild boar samples containing high HEV genome copy numbers. The isolates may be used further to study the zoonotic potential of wild boar-derived HEV subtypes.Katja Schilling-LoefflerOliver Viera-SeguraVictor Max CormanJulia SchneiderAshish K. GadicherlaUlrich SchotteReimar JohneMDPI AGarticlehepatitis E viruswild boarcell culturehypervariable regionwhole genome sequencingzoonosisBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2302, p 2302 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hepatitis E virus wild boar cell culture hypervariable region whole genome sequencing zoonosis Biology (General) QH301-705.5
spellingShingle	hepatitis E virus wild boar cell culture hypervariable region whole genome sequencing zoonosis Biology (General) QH301-705.5 Katja Schilling-Loeffler Oliver Viera-Segura Victor Max Corman Julia Schneider Ashish K. Gadicherla Ulrich Schotte Reimar Johne Cell Culture Isolation and Whole Genome Characterization of Hepatitis E Virus Strains from Wild Boars in Germany
description	Infection with hepatitis E virus (HEV) can cause acute and chronic hepatitis in humans. The HEV genotype 3 can be zoonotically transmitted from animals to humans, with wild boars representing an important reservoir species. Cell culture isolation of HEV is generally difficult and mainly described for human isolates so far. Here, five sera and five liver samples from HEV-RNA-positive wild boar samples were inoculated onto PLC/PRF/5 cells, incubated for 3 months and thereafter passaged for additional 6 weeks. As demonstrated by RT-qPCR, immunofluorescence and immune electron microscopy, virus was successfully isolated from two liver samples, which originally contained high HEV genome copy numbers. Both isolates showed slower growth than the culture-adapted HEV strain 47832c. In contrast to this strain, the isolated strains had no insertions in their hypervariable genome region. Next generation sequencing using an HEV sequence-enriched library enabled full genome sequencing. Strain Wb108/17 belongs to subtype 3f and strain Wb257/17 to a tentative novel subtype recently described in Italian wild boars. The results indicate that HEV can be successfully isolated in cell culture from wild boar samples containing high HEV genome copy numbers. The isolates may be used further to study the zoonotic potential of wild boar-derived HEV subtypes.
format	article
author	Katja Schilling-Loeffler Oliver Viera-Segura Victor Max Corman Julia Schneider Ashish K. Gadicherla Ulrich Schotte Reimar Johne
author_facet	Katja Schilling-Loeffler Oliver Viera-Segura Victor Max Corman Julia Schneider Ashish K. Gadicherla Ulrich Schotte Reimar Johne
author_sort	Katja Schilling-Loeffler
title	Cell Culture Isolation and Whole Genome Characterization of Hepatitis E Virus Strains from Wild Boars in Germany
title_short	Cell Culture Isolation and Whole Genome Characterization of Hepatitis E Virus Strains from Wild Boars in Germany
title_full	Cell Culture Isolation and Whole Genome Characterization of Hepatitis E Virus Strains from Wild Boars in Germany
title_fullStr	Cell Culture Isolation and Whole Genome Characterization of Hepatitis E Virus Strains from Wild Boars in Germany
title_full_unstemmed	Cell Culture Isolation and Whole Genome Characterization of Hepatitis E Virus Strains from Wild Boars in Germany
title_sort	cell culture isolation and whole genome characterization of hepatitis e virus strains from wild boars in germany
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/8ef4c5a954544adf93f9f3c1acb9f598
work_keys_str_mv	AT katjaschillingloeffler cellcultureisolationandwholegenomecharacterizationofhepatitisevirusstrainsfromwildboarsingermany AT olivervierasegura cellcultureisolationandwholegenomecharacterizationofhepatitisevirusstrainsfromwildboarsingermany AT victormaxcorman cellcultureisolationandwholegenomecharacterizationofhepatitisevirusstrainsfromwildboarsingermany AT juliaschneider cellcultureisolationandwholegenomecharacterizationofhepatitisevirusstrainsfromwildboarsingermany AT ashishkgadicherla cellcultureisolationandwholegenomecharacterizationofhepatitisevirusstrainsfromwildboarsingermany AT ulrichschotte cellcultureisolationandwholegenomecharacterizationofhepatitisevirusstrainsfromwildboarsingermany AT reimarjohne cellcultureisolationandwholegenomecharacterizationofhepatitisevirusstrainsfromwildboarsingermany
_version_	1718411211158061056

Cell Culture Isolation and Whole Genome Characterization of Hepatitis E Virus Strains from Wild Boars in Germany

Ejemplares similares