ACR11 modulates levels of reactive oxygen species and salicylic acid-associated defense response in Arabidopsis

Abstract The ACT domain (aspartate kinase, chorismate mutase and TyrA), an allosteric effector binding domain, is commonly found in amino acid metabolic enzymes. In addition to ACT domain-containing enzymes, plants have a novel family of ACT domain repeat (ACR) proteins, which do not contain any rec...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shashi Kant Singh, Tzu-Ying Sung, Tsui-Yun Chung, Shao-Yu Lin, Sang-Chu Lin, Jo-Chien Liao, Wei-Yu Hsieh, Ming-Hsiun Hsieh
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8ef539a3f5f944f0b2640f3ec0334312
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8ef539a3f5f944f0b2640f3ec0334312
record_format	dspace
spelling	oai:doaj.org-article:8ef539a3f5f944f0b2640f3ec03343122021-12-02T15:07:51ZACR11 modulates levels of reactive oxygen species and salicylic acid-associated defense response in Arabidopsis10.1038/s41598-018-30304-02045-2322https://doaj.org/article/8ef539a3f5f944f0b2640f3ec03343122018-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-30304-0https://doaj.org/toc/2045-2322Abstract The ACT domain (aspartate kinase, chorismate mutase and TyrA), an allosteric effector binding domain, is commonly found in amino acid metabolic enzymes. In addition to ACT domain-containing enzymes, plants have a novel family of ACT domain repeat (ACR) proteins, which do not contain any recognizable catalytic domain. Arabidopsis has 12 ACR proteins, whose functions are largely unknown. To study the functions of Arabidopsis ACR11, we have characterized two independent T-DNA insertion mutants, acr11-2 and acr11-3. RNA gel-blot analysis revealed that the expression of wild-type ACR11 transcripts was not detectable in the acr11 mutants. Interestingly, a lesion-mimic phenotype occurs in some rosette leaves of the acr11 mutants. In addition, high levels of reactive oxygen species (ROS), salicylic acid (SA), and callose accumulate in the mutant leaves when grown under normal conditions. The expression of several SA marker genes and the key SA biosynthetic gene ISOCHORISMATE SYNTHASE1 is up-regulated in the acr11 mutants. Furthermore, the acr11 mutants are more resistant to the infection of bacterial pathogen Pseudomonas syringae pathovar tomato DC3000. These results suggest that ACR11 may be directly or indirectly involved in the regulation of ROS and SA accumulation, which in turn modulates SA-associated defense responses and disease resistance in Arabidopsis.Shashi Kant SinghTzu-Ying SungTsui-Yun ChungShao-Yu LinSang-Chu LinJo-Chien LiaoWei-Yu HsiehMing-Hsiun HsiehNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shashi Kant Singh Tzu-Ying Sung Tsui-Yun Chung Shao-Yu Lin Sang-Chu Lin Jo-Chien Liao Wei-Yu Hsieh Ming-Hsiun Hsieh ACR11 modulates levels of reactive oxygen species and salicylic acid-associated defense response in Arabidopsis
description	Abstract The ACT domain (aspartate kinase, chorismate mutase and TyrA), an allosteric effector binding domain, is commonly found in amino acid metabolic enzymes. In addition to ACT domain-containing enzymes, plants have a novel family of ACT domain repeat (ACR) proteins, which do not contain any recognizable catalytic domain. Arabidopsis has 12 ACR proteins, whose functions are largely unknown. To study the functions of Arabidopsis ACR11, we have characterized two independent T-DNA insertion mutants, acr11-2 and acr11-3. RNA gel-blot analysis revealed that the expression of wild-type ACR11 transcripts was not detectable in the acr11 mutants. Interestingly, a lesion-mimic phenotype occurs in some rosette leaves of the acr11 mutants. In addition, high levels of reactive oxygen species (ROS), salicylic acid (SA), and callose accumulate in the mutant leaves when grown under normal conditions. The expression of several SA marker genes and the key SA biosynthetic gene ISOCHORISMATE SYNTHASE1 is up-regulated in the acr11 mutants. Furthermore, the acr11 mutants are more resistant to the infection of bacterial pathogen Pseudomonas syringae pathovar tomato DC3000. These results suggest that ACR11 may be directly or indirectly involved in the regulation of ROS and SA accumulation, which in turn modulates SA-associated defense responses and disease resistance in Arabidopsis.
format	article
author	Shashi Kant Singh Tzu-Ying Sung Tsui-Yun Chung Shao-Yu Lin Sang-Chu Lin Jo-Chien Liao Wei-Yu Hsieh Ming-Hsiun Hsieh
author_facet	Shashi Kant Singh Tzu-Ying Sung Tsui-Yun Chung Shao-Yu Lin Sang-Chu Lin Jo-Chien Liao Wei-Yu Hsieh Ming-Hsiun Hsieh
author_sort	Shashi Kant Singh
title	ACR11 modulates levels of reactive oxygen species and salicylic acid-associated defense response in Arabidopsis
title_short	ACR11 modulates levels of reactive oxygen species and salicylic acid-associated defense response in Arabidopsis
title_full	ACR11 modulates levels of reactive oxygen species and salicylic acid-associated defense response in Arabidopsis
title_fullStr	ACR11 modulates levels of reactive oxygen species and salicylic acid-associated defense response in Arabidopsis
title_full_unstemmed	ACR11 modulates levels of reactive oxygen species and salicylic acid-associated defense response in Arabidopsis
title_sort	acr11 modulates levels of reactive oxygen species and salicylic acid-associated defense response in arabidopsis
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/8ef539a3f5f944f0b2640f3ec0334312
work_keys_str_mv	AT shashikantsingh acr11modulateslevelsofreactiveoxygenspeciesandsalicylicacidassociateddefenseresponseinarabidopsis AT tzuyingsung acr11modulateslevelsofreactiveoxygenspeciesandsalicylicacidassociateddefenseresponseinarabidopsis AT tsuiyunchung acr11modulateslevelsofreactiveoxygenspeciesandsalicylicacidassociateddefenseresponseinarabidopsis AT shaoyulin acr11modulateslevelsofreactiveoxygenspeciesandsalicylicacidassociateddefenseresponseinarabidopsis AT sangchulin acr11modulateslevelsofreactiveoxygenspeciesandsalicylicacidassociateddefenseresponseinarabidopsis AT jochienliao acr11modulateslevelsofreactiveoxygenspeciesandsalicylicacidassociateddefenseresponseinarabidopsis AT weiyuhsieh acr11modulateslevelsofreactiveoxygenspeciesandsalicylicacidassociateddefenseresponseinarabidopsis AT minghsiunhsieh acr11modulateslevelsofreactiveoxygenspeciesandsalicylicacidassociateddefenseresponseinarabidopsis
_version_	1718388402778275840

ACR11 modulates levels of reactive oxygen species and salicylic acid-associated defense response in Arabidopsis

Ejemplares similares