Indicators of the molecular pathogenesis of virulent Newcastle disease virus in chickens revealed by transcriptomic profiling of spleen

Abstract Newcastle disease virus (NDV) has caused significant outbreaks in South-East Asia, particularly in Indonesia in recent years. Recently emerged genotype VII NDVs (NDV-GVII) have shifted their tropism from gastrointestinal/respiratory tropism to a lymphotropic virus, invading lymphoid organs...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mohammad Rabiei, Wai Yee Low, Yan Ren, Mohamad Indro Cahyono, Phuong Thi Kim Doan, Indi Dharmayanti, Eleonora Dal Grande, Farhid Hemmatzadeh
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8ef630892335491c8ce2cfb979e8c9ba
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8ef630892335491c8ce2cfb979e8c9ba
record_format	dspace
spelling	oai:doaj.org-article:8ef630892335491c8ce2cfb979e8c9ba2021-12-02T15:29:02ZIndicators of the molecular pathogenesis of virulent Newcastle disease virus in chickens revealed by transcriptomic profiling of spleen10.1038/s41598-021-96929-w2045-2322https://doaj.org/article/8ef630892335491c8ce2cfb979e8c9ba2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96929-whttps://doaj.org/toc/2045-2322Abstract Newcastle disease virus (NDV) has caused significant outbreaks in South-East Asia, particularly in Indonesia in recent years. Recently emerged genotype VII NDVs (NDV-GVII) have shifted their tropism from gastrointestinal/respiratory tropism to a lymphotropic virus, invading lymphoid organs including spleen and bursa of Fabricius to cause profound lymphoid depletion. In this study, we aimed to identify candidate genes and biological pathways that contribute to the disease caused by this velogenic NDV-GVII. A transcriptomic analysis based on RNA-Seq of spleen was performed in chickens challenged with NDV-GVII and a control group. In total, 6361 genes were differentially expressed that included 3506 up-regulated genes and 2855 down-regulated genes. Real-Time PCR of ten selected genes validated the RNA-Seq results as the correlation between them is 0.98. Functional and network analysis of Differentially Expressed Genes (DEGs) showed altered regulation of ElF2 signalling, mTOR signalling, proliferation of cells of the lymphoid system, signalling by Rho family GTPases and synaptogenesis signalling in spleen. We have also identified modified expression of IFIT5, PI3K, AGT and PLP1 genes in NDV-GVII infected chickens. Our findings in activation of autophagy-mediated cell death, lymphotropic and synaptogenesis signalling pathways provide new insights into the molecular pathogenesis of this newly emerged NDV-GVII.Mohammad RabieiWai Yee LowYan RenMohamad Indro CahyonoPhuong Thi Kim DoanIndi DharmayantiEleonora Dal GrandeFarhid HemmatzadehNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mohammad Rabiei Wai Yee Low Yan Ren Mohamad Indro Cahyono Phuong Thi Kim Doan Indi Dharmayanti Eleonora Dal Grande Farhid Hemmatzadeh Indicators of the molecular pathogenesis of virulent Newcastle disease virus in chickens revealed by transcriptomic profiling of spleen
description	Abstract Newcastle disease virus (NDV) has caused significant outbreaks in South-East Asia, particularly in Indonesia in recent years. Recently emerged genotype VII NDVs (NDV-GVII) have shifted their tropism from gastrointestinal/respiratory tropism to a lymphotropic virus, invading lymphoid organs including spleen and bursa of Fabricius to cause profound lymphoid depletion. In this study, we aimed to identify candidate genes and biological pathways that contribute to the disease caused by this velogenic NDV-GVII. A transcriptomic analysis based on RNA-Seq of spleen was performed in chickens challenged with NDV-GVII and a control group. In total, 6361 genes were differentially expressed that included 3506 up-regulated genes and 2855 down-regulated genes. Real-Time PCR of ten selected genes validated the RNA-Seq results as the correlation between them is 0.98. Functional and network analysis of Differentially Expressed Genes (DEGs) showed altered regulation of ElF2 signalling, mTOR signalling, proliferation of cells of the lymphoid system, signalling by Rho family GTPases and synaptogenesis signalling in spleen. We have also identified modified expression of IFIT5, PI3K, AGT and PLP1 genes in NDV-GVII infected chickens. Our findings in activation of autophagy-mediated cell death, lymphotropic and synaptogenesis signalling pathways provide new insights into the molecular pathogenesis of this newly emerged NDV-GVII.
format	article
author	Mohammad Rabiei Wai Yee Low Yan Ren Mohamad Indro Cahyono Phuong Thi Kim Doan Indi Dharmayanti Eleonora Dal Grande Farhid Hemmatzadeh
author_facet	Mohammad Rabiei Wai Yee Low Yan Ren Mohamad Indro Cahyono Phuong Thi Kim Doan Indi Dharmayanti Eleonora Dal Grande Farhid Hemmatzadeh
author_sort	Mohammad Rabiei
title	Indicators of the molecular pathogenesis of virulent Newcastle disease virus in chickens revealed by transcriptomic profiling of spleen
title_short	Indicators of the molecular pathogenesis of virulent Newcastle disease virus in chickens revealed by transcriptomic profiling of spleen
title_full	Indicators of the molecular pathogenesis of virulent Newcastle disease virus in chickens revealed by transcriptomic profiling of spleen
title_fullStr	Indicators of the molecular pathogenesis of virulent Newcastle disease virus in chickens revealed by transcriptomic profiling of spleen
title_full_unstemmed	Indicators of the molecular pathogenesis of virulent Newcastle disease virus in chickens revealed by transcriptomic profiling of spleen
title_sort	indicators of the molecular pathogenesis of virulent newcastle disease virus in chickens revealed by transcriptomic profiling of spleen
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8ef630892335491c8ce2cfb979e8c9ba
work_keys_str_mv	AT mohammadrabiei indicatorsofthemolecularpathogenesisofvirulentnewcastlediseasevirusinchickensrevealedbytranscriptomicprofilingofspleen AT waiyeelow indicatorsofthemolecularpathogenesisofvirulentnewcastlediseasevirusinchickensrevealedbytranscriptomicprofilingofspleen AT yanren indicatorsofthemolecularpathogenesisofvirulentnewcastlediseasevirusinchickensrevealedbytranscriptomicprofilingofspleen AT mohamadindrocahyono indicatorsofthemolecularpathogenesisofvirulentnewcastlediseasevirusinchickensrevealedbytranscriptomicprofilingofspleen AT phuongthikimdoan indicatorsofthemolecularpathogenesisofvirulentnewcastlediseasevirusinchickensrevealedbytranscriptomicprofilingofspleen AT indidharmayanti indicatorsofthemolecularpathogenesisofvirulentnewcastlediseasevirusinchickensrevealedbytranscriptomicprofilingofspleen AT eleonoradalgrande indicatorsofthemolecularpathogenesisofvirulentnewcastlediseasevirusinchickensrevealedbytranscriptomicprofilingofspleen AT farhidhemmatzadeh indicatorsofthemolecularpathogenesisofvirulentnewcastlediseasevirusinchickensrevealedbytranscriptomicprofilingofspleen
_version_	1718387154022825984

Indicators of the molecular pathogenesis of virulent Newcastle disease virus in chickens revealed by transcriptomic profiling of spleen

Ejemplares similares