Polyimide mesh-based sample holder with irregular crystal mounting holes for fixed-target serial crystallography

Abstract The serial crystallography (SX) technique enables the determination of the room-temperature structure of a macromolecule while causing minimal radiation damage, as well as the visualization of the molecular dynamics by time-resolved studies. The fixed-target (FT) scanning approach is one me...

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Autores principales:	Ki Hyun Nam, Jihan Kim, Yunje Cho
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8efaf6bc55fb4cf6ba4a6bda82f2385d
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spelling	oai:doaj.org-article:8efaf6bc55fb4cf6ba4a6bda82f2385d2021-12-02T18:02:44ZPolyimide mesh-based sample holder with irregular crystal mounting holes for fixed-target serial crystallography10.1038/s41598-021-92687-x2045-2322https://doaj.org/article/8efaf6bc55fb4cf6ba4a6bda82f2385d2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92687-xhttps://doaj.org/toc/2045-2322Abstract The serial crystallography (SX) technique enables the determination of the room-temperature structure of a macromolecule while causing minimal radiation damage, as well as the visualization of the molecular dynamics by time-resolved studies. The fixed-target (FT) scanning approach is one method for SX sample delivery that minimizes sample consumption and minimizes physical damage to crystals during data collection. Settling of the crystals on the sample holder in random orientation is important for complete three dimensional data collection. To increase the random orientation of crystals on the sample holder, we developed a polyimide mesh-based sample holder with irregular crystal mounting holes for FT-SX. The polyimide mesh was fabricated using a picosecond laser. Each hole in the polyimide mesh has irregularly shaped holes because of laser thermal damage, which may cause more crystals to settle at random orientations compared to regular shaped sample holders. A crystal sample was spread onto a polyimide-mesh, and a polyimide film was added to both sides to prevent dehydration. Using this sample holder, FT-SX was performed at synchrotron and determined the room-temperature lysozyme structure at 1.65 Å. The polyimide mesh with irregularly shaped holes will allow for expanded applications in sample delivery for FT-SX experiments.Ki Hyun NamJihan KimYunje ChoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ki Hyun Nam Jihan Kim Yunje Cho Polyimide mesh-based sample holder with irregular crystal mounting holes for fixed-target serial crystallography
description	Abstract The serial crystallography (SX) technique enables the determination of the room-temperature structure of a macromolecule while causing minimal radiation damage, as well as the visualization of the molecular dynamics by time-resolved studies. The fixed-target (FT) scanning approach is one method for SX sample delivery that minimizes sample consumption and minimizes physical damage to crystals during data collection. Settling of the crystals on the sample holder in random orientation is important for complete three dimensional data collection. To increase the random orientation of crystals on the sample holder, we developed a polyimide mesh-based sample holder with irregular crystal mounting holes for FT-SX. The polyimide mesh was fabricated using a picosecond laser. Each hole in the polyimide mesh has irregularly shaped holes because of laser thermal damage, which may cause more crystals to settle at random orientations compared to regular shaped sample holders. A crystal sample was spread onto a polyimide-mesh, and a polyimide film was added to both sides to prevent dehydration. Using this sample holder, FT-SX was performed at synchrotron and determined the room-temperature lysozyme structure at 1.65 Å. The polyimide mesh with irregularly shaped holes will allow for expanded applications in sample delivery for FT-SX experiments.
format	article
author	Ki Hyun Nam Jihan Kim Yunje Cho
author_facet	Ki Hyun Nam Jihan Kim Yunje Cho
author_sort	Ki Hyun Nam
title	Polyimide mesh-based sample holder with irregular crystal mounting holes for fixed-target serial crystallography
title_short	Polyimide mesh-based sample holder with irregular crystal mounting holes for fixed-target serial crystallography
title_full	Polyimide mesh-based sample holder with irregular crystal mounting holes for fixed-target serial crystallography
title_fullStr	Polyimide mesh-based sample holder with irregular crystal mounting holes for fixed-target serial crystallography
title_full_unstemmed	Polyimide mesh-based sample holder with irregular crystal mounting holes for fixed-target serial crystallography
title_sort	polyimide mesh-based sample holder with irregular crystal mounting holes for fixed-target serial crystallography
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8efaf6bc55fb4cf6ba4a6bda82f2385d
work_keys_str_mv	AT kihyunnam polyimidemeshbasedsampleholderwithirregularcrystalmountingholesforfixedtargetserialcrystallography AT jihankim polyimidemeshbasedsampleholderwithirregularcrystalmountingholesforfixedtargetserialcrystallography AT yunjecho polyimidemeshbasedsampleholderwithirregularcrystalmountingholesforfixedtargetserialcrystallography
_version_	1718378880510722048

Polyimide mesh-based sample holder with irregular crystal mounting holes for fixed-target serial crystallography

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