Quantitative analysis of galactose using LDI-TOF MS based on a TiO2 nanowire chip

Abstract A novel method for quantifying galactose was developed to serve as a newborn screening test for galactosemia using laser desorption/ionization time-of-flight (LDI-TOF) mass spectrometry (MS) with a TiO2 nanowire chip. Herein, phosphate citrate buffer, serum, and dried blood spot (DBS) were...

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Autores principales:	Joo-Yoon Noh, Moon-Ju Kim, Mira Kim, Jo-Il Kim, Jong-Min Park, Tae Gyeong Yun, Min-Jung Kang, Jae-Chul Pyun
Formato:	article
Lenguaje:	EN
Publicado:	SpringerOpen 2021
Materias:	Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry TiO2 nanowire chip Galactose Reduction power o-phenylene diamine 2,3-diaminophenazine Chemistry QD1-999 Analytical chemistry QD71-142
Acceso en línea:	https://doaj.org/article/8efec2d00a2f4013a7648479b6d800bc
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spelling	oai:doaj.org-article:8efec2d00a2f4013a7648479b6d800bc2021-11-07T12:07:11ZQuantitative analysis of galactose using LDI-TOF MS based on a TiO2 nanowire chip10.1186/s40543-021-00300-72093-3371https://doaj.org/article/8efec2d00a2f4013a7648479b6d800bc2021-11-01T00:00:00Zhttps://doi.org/10.1186/s40543-021-00300-7https://doaj.org/toc/2093-3371Abstract A novel method for quantifying galactose was developed to serve as a newborn screening test for galactosemia using laser desorption/ionization time-of-flight (LDI-TOF) mass spectrometry (MS) with a TiO2 nanowire chip. Herein, phosphate citrate buffer, serum, and dried blood spot (DBS) were employed for the quantitative analysis of galactose. To quantitatively analyze galactose, its reduction potential was used to oxidize o-phenylene diamine (OPD) into 2,3-diaminophenazine (DA), which were both detected using LDI-TOF MS with a TiO2 nanowire chip according to the concentration of galactose. The reproducibility and the interference of glucose were determined to demonstrate the applicability of this method. Moreover, mixtures of galactose, phenylalanine, and 17 α-OHP were analyzed to determine the interference induced by other biomarkers of metabolic disorders. The OPD oxidation of galactose was found to be selectively achieved under high-glucose conditions, similar to human blood, thereby showing good reproducibility. The intensities of the mass peaks of OPD and DA based on LDI-TOF MS with a TiO2 nanowire chip were linearly correlated in the galactose concentration range of 57.2–220.0 μg/mL (r 2 = 0.999 and 0.950, respectively) for serum samples and 52.5–220.0 μg/mL (r 2 = 0.993 and 0.985, respectively) for DBS after methanol precipitation/extraction. The enzyme immunoassay and LDI-TOF MS analysis results were statistically analyzed, and a mixture of phenylalanine, 17 α-OHP, and galactose was simultaneously investigated quantitatively at the cutoff level.Joo-Yoon NohMoon-Ju KimMira KimJo-Il KimJong-Min ParkTae Gyeong YunMin-Jung KangJae-Chul PyunSpringerOpenarticleMatrix-assisted laser desorption/ionization time-of-flight mass spectrometryTiO2 nanowire chipGalactoseReduction powero-phenylene diamine2,3-diaminophenazineChemistryQD1-999Analytical chemistryQD71-142ENJournal of Analytical Science and Technology, Vol 12, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry TiO2 nanowire chip Galactose Reduction power o-phenylene diamine 2,3-diaminophenazine Chemistry QD1-999 Analytical chemistry QD71-142
spellingShingle	Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry TiO2 nanowire chip Galactose Reduction power o-phenylene diamine 2,3-diaminophenazine Chemistry QD1-999 Analytical chemistry QD71-142 Joo-Yoon Noh Moon-Ju Kim Mira Kim Jo-Il Kim Jong-Min Park Tae Gyeong Yun Min-Jung Kang Jae-Chul Pyun Quantitative analysis of galactose using LDI-TOF MS based on a TiO2 nanowire chip
description	Abstract A novel method for quantifying galactose was developed to serve as a newborn screening test for galactosemia using laser desorption/ionization time-of-flight (LDI-TOF) mass spectrometry (MS) with a TiO2 nanowire chip. Herein, phosphate citrate buffer, serum, and dried blood spot (DBS) were employed for the quantitative analysis of galactose. To quantitatively analyze galactose, its reduction potential was used to oxidize o-phenylene diamine (OPD) into 2,3-diaminophenazine (DA), which were both detected using LDI-TOF MS with a TiO2 nanowire chip according to the concentration of galactose. The reproducibility and the interference of glucose were determined to demonstrate the applicability of this method. Moreover, mixtures of galactose, phenylalanine, and 17 α-OHP were analyzed to determine the interference induced by other biomarkers of metabolic disorders. The OPD oxidation of galactose was found to be selectively achieved under high-glucose conditions, similar to human blood, thereby showing good reproducibility. The intensities of the mass peaks of OPD and DA based on LDI-TOF MS with a TiO2 nanowire chip were linearly correlated in the galactose concentration range of 57.2–220.0 μg/mL (r 2 = 0.999 and 0.950, respectively) for serum samples and 52.5–220.0 μg/mL (r 2 = 0.993 and 0.985, respectively) for DBS after methanol precipitation/extraction. The enzyme immunoassay and LDI-TOF MS analysis results were statistically analyzed, and a mixture of phenylalanine, 17 α-OHP, and galactose was simultaneously investigated quantitatively at the cutoff level.
format	article
author	Joo-Yoon Noh Moon-Ju Kim Mira Kim Jo-Il Kim Jong-Min Park Tae Gyeong Yun Min-Jung Kang Jae-Chul Pyun
author_facet	Joo-Yoon Noh Moon-Ju Kim Mira Kim Jo-Il Kim Jong-Min Park Tae Gyeong Yun Min-Jung Kang Jae-Chul Pyun
author_sort	Joo-Yoon Noh
title	Quantitative analysis of galactose using LDI-TOF MS based on a TiO2 nanowire chip
title_short	Quantitative analysis of galactose using LDI-TOF MS based on a TiO2 nanowire chip
title_full	Quantitative analysis of galactose using LDI-TOF MS based on a TiO2 nanowire chip
title_fullStr	Quantitative analysis of galactose using LDI-TOF MS based on a TiO2 nanowire chip
title_full_unstemmed	Quantitative analysis of galactose using LDI-TOF MS based on a TiO2 nanowire chip
title_sort	quantitative analysis of galactose using ldi-tof ms based on a tio2 nanowire chip
publisher	SpringerOpen
publishDate	2021
url	https://doaj.org/article/8efec2d00a2f4013a7648479b6d800bc
work_keys_str_mv	AT jooyoonnoh quantitativeanalysisofgalactoseusinglditofmsbasedonatio2nanowirechip AT moonjukim quantitativeanalysisofgalactoseusinglditofmsbasedonatio2nanowirechip AT mirakim quantitativeanalysisofgalactoseusinglditofmsbasedonatio2nanowirechip AT joilkim quantitativeanalysisofgalactoseusinglditofmsbasedonatio2nanowirechip AT jongminpark quantitativeanalysisofgalactoseusinglditofmsbasedonatio2nanowirechip AT taegyeongyun quantitativeanalysisofgalactoseusinglditofmsbasedonatio2nanowirechip AT minjungkang quantitativeanalysisofgalactoseusinglditofmsbasedonatio2nanowirechip AT jaechulpyun quantitativeanalysisofgalactoseusinglditofmsbasedonatio2nanowirechip
_version_	1718443560847540224

Quantitative analysis of galactose using LDI-TOF MS based on a TiO2 nanowire chip

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