CMCE Propolis Extract Improve Caspase 3 Expressions of The Hepatocytes and IL-6 Levels in Rats Exposed with CCl4

Introduction: Liver plays an important role in the metabolic processes possibly exposed to the toxic materials. One of those hepatotoxic substances is carbon tetrachloride. The propolis extract contains the balsamic active substances, CAPE compounds and flavonoids. Flavonoids can prevent from the ap...

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Autores principales:	Chalid Firdaus, Chodidjah Chodidjah, Taufiqurrachman Nasihun
Formato:	article
Lenguaje:	EN
Publicado:	Universitas Sultan Agung Semarang 2021
Materias:	propolis extract (cmce method), caspase 3 expression in hepatocytes, il-6 levels. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/8f0cda5986d741439456fdee8267329e
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spelling	oai:doaj.org-article:8f0cda5986d741439456fdee8267329e2021-11-25T13:16:44ZCMCE Propolis Extract Improve Caspase 3 Expressions of The Hepatocytes and IL-6 Levels in Rats Exposed with CCl42085-15452339-093X10.30659/sainsmed.v11i2.5190https://doaj.org/article/8f0cda5986d741439456fdee8267329e2021-01-01T00:00:00Zhttp://jurnal.unissula.ac.id/index.php/sainsmedika/article/view/5190https://doaj.org/toc/2085-1545https://doaj.org/toc/2339-093XIntroduction: Liver plays an important role in the metabolic processes possibly exposed to the toxic materials. One of those hepatotoxic substances is carbon tetrachloride. The propolis extract contains the balsamic active substances, CAPE compounds and flavonoids. Flavonoids can prevent from the apoptosis and reduce the inflammation. Objective: To validate propolis extract’s (CMCE method) ability in improving caspase 3 expressions of hepatocytes and IL-6 levels in Wistar male rats induced with carbon tetrachloride. Methods: An experimental study with a post-test only control group design applied to the study. The research subjects were 28 male Wistar rats, divided into four groups. The negative control group (CN-G) was only injected with CCl4, while the experimental groups were administered with the CMCE propolis at the dosages of 3.6 mg/200g (CM3-G), 7.2 mg/200g (CM7-G), and 14.4 mg/200g (CM14-G). The CMCE propolis extract was administered for 14 days and on day 14, the CCl4 was then given. The caspase 3 expression of hepatocytes was measured using HE liver cell preparations, while the IL-6 levels were measured using ELISA method. Results: The results of Mann-Whitney U statistical analysis showed that the hepatocytes’ caspase 3 expressions in CM14-G (2.77 ± 0.531), CM7-G (3.14 ± 0.378), and CM3-G (4.22 ± 0.690) were lower than those in CN-G (5.43 ± 0.535), p<0.05. Meanwhile, the Post Hoc LSD analysis Results showed that IL-6 levels in CM14-G (55.032 ± 9.336), CM7-G (78.362 ± 8.313), and CM3-G (114.975 ± 10.359) were lower those in CN-G (180.301 ± 5.428), p<0.05. Conclusion: the administration of propolis extract (CMCE method) improve the caspase 3 expressions of hepatocytes and Il-6 levels in Wistar male rats induced with carbon tetrachloride.Chalid FirdausChodidjah ChodidjahTaufiqurrachman NasihunUniversitas Sultan Agung Semarangarticlepropolis extract (cmce method), caspase 3 expression in hepatocytes, il-6 levels.Medicine (General)R5-920ENSains Medika, Vol 11, Iss 2, Pp 67-73 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	propolis extract (cmce method), caspase 3 expression in hepatocytes, il-6 levels. Medicine (General) R5-920
spellingShingle	propolis extract (cmce method), caspase 3 expression in hepatocytes, il-6 levels. Medicine (General) R5-920 Chalid Firdaus Chodidjah Chodidjah Taufiqurrachman Nasihun CMCE Propolis Extract Improve Caspase 3 Expressions of The Hepatocytes and IL-6 Levels in Rats Exposed with CCl4
description	Introduction: Liver plays an important role in the metabolic processes possibly exposed to the toxic materials. One of those hepatotoxic substances is carbon tetrachloride. The propolis extract contains the balsamic active substances, CAPE compounds and flavonoids. Flavonoids can prevent from the apoptosis and reduce the inflammation. Objective: To validate propolis extract’s (CMCE method) ability in improving caspase 3 expressions of hepatocytes and IL-6 levels in Wistar male rats induced with carbon tetrachloride. Methods: An experimental study with a post-test only control group design applied to the study. The research subjects were 28 male Wistar rats, divided into four groups. The negative control group (CN-G) was only injected with CCl4, while the experimental groups were administered with the CMCE propolis at the dosages of 3.6 mg/200g (CM3-G), 7.2 mg/200g (CM7-G), and 14.4 mg/200g (CM14-G). The CMCE propolis extract was administered for 14 days and on day 14, the CCl4 was then given. The caspase 3 expression of hepatocytes was measured using HE liver cell preparations, while the IL-6 levels were measured using ELISA method. Results: The results of Mann-Whitney U statistical analysis showed that the hepatocytes’ caspase 3 expressions in CM14-G (2.77 ± 0.531), CM7-G (3.14 ± 0.378), and CM3-G (4.22 ± 0.690) were lower than those in CN-G (5.43 ± 0.535), p<0.05. Meanwhile, the Post Hoc LSD analysis Results showed that IL-6 levels in CM14-G (55.032 ± 9.336), CM7-G (78.362 ± 8.313), and CM3-G (114.975 ± 10.359) were lower those in CN-G (180.301 ± 5.428), p<0.05. Conclusion: the administration of propolis extract (CMCE method) improve the caspase 3 expressions of hepatocytes and Il-6 levels in Wistar male rats induced with carbon tetrachloride.
format	article
author	Chalid Firdaus Chodidjah Chodidjah Taufiqurrachman Nasihun
author_facet	Chalid Firdaus Chodidjah Chodidjah Taufiqurrachman Nasihun
author_sort	Chalid Firdaus
title	CMCE Propolis Extract Improve Caspase 3 Expressions of The Hepatocytes and IL-6 Levels in Rats Exposed with CCl4
title_short	CMCE Propolis Extract Improve Caspase 3 Expressions of The Hepatocytes and IL-6 Levels in Rats Exposed with CCl4
title_full	CMCE Propolis Extract Improve Caspase 3 Expressions of The Hepatocytes and IL-6 Levels in Rats Exposed with CCl4
title_fullStr	CMCE Propolis Extract Improve Caspase 3 Expressions of The Hepatocytes and IL-6 Levels in Rats Exposed with CCl4
title_full_unstemmed	CMCE Propolis Extract Improve Caspase 3 Expressions of The Hepatocytes and IL-6 Levels in Rats Exposed with CCl4
title_sort	cmce propolis extract improve caspase 3 expressions of the hepatocytes and il-6 levels in rats exposed with ccl4
publisher	Universitas Sultan Agung Semarang
publishDate	2021
url	https://doaj.org/article/8f0cda5986d741439456fdee8267329e
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CMCE Propolis Extract Improve Caspase 3 Expressions of The Hepatocytes and IL-6 Levels in Rats Exposed with CCl4

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