Proteomic and phosphoproteomic profiling of shammah induced signaling in oral keratinocytes

Abstract Shammah is a smokeless tobacco product often mixed with lime, ash, black pepper and flavorings. Exposure to shammah has been linked with dental diseases and oral squamous cell carcinoma. There is limited literature on the prevalence of shammah and its role in pathobiology of oral cancer. In...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shankargouda Patil, Mohd Younis Bhat, Jayshree Advani, Sonali V. Mohan, Niraj Babu, Keshava K. Datta, Tejaswini Subbannayya, Pavithra Rajagopalan, Firdous A. Bhat, Nezar Al-hebshi, David Sidransky, Harsha Gowda, Aditi Chatterjee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8f0e8442e1844fe5a525d733299b371e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8f0e8442e1844fe5a525d733299b371e
record_format	dspace
spelling	oai:doaj.org-article:8f0e8442e1844fe5a525d733299b371e2021-12-02T17:15:33ZProteomic and phosphoproteomic profiling of shammah induced signaling in oral keratinocytes10.1038/s41598-021-88345-x2045-2322https://doaj.org/article/8f0e8442e1844fe5a525d733299b371e2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88345-xhttps://doaj.org/toc/2045-2322Abstract Shammah is a smokeless tobacco product often mixed with lime, ash, black pepper and flavorings. Exposure to shammah has been linked with dental diseases and oral squamous cell carcinoma. There is limited literature on the prevalence of shammah and its role in pathobiology of oral cancer. In this study, we developed a cellular model to understand the effect of chronic shammah exposure on oral keratinocytes. Chronic exposure to shammah resulted in increased proliferation and invasiveness of non-transformed oral keratinocytes. Quantitative proteomics of shammah treated cells compared to untreated cells led to quantification of 4712 proteins of which 402 were found to be significantly altered. In addition, phosphoproteomics analysis of shammah treated cells compared to untreated revealed hyperphosphorylation of 36 proteins and hypophosphorylation of 83 proteins (twofold, p-value ≤ 0.05). Bioinformatics analysis of significantly altered proteins showed enrichment of proteins involved in extracellular matrix interactions, necroptosis and peroxisome mediated fatty acid oxidation. Kinase-Substrate Enrichment Analysis showed significant increase in activity of kinases such as ROCK1, RAF1, PRKCE and HIPK2 in shammah treated cells. These results provide better understanding of how shammah transforms non-neoplastic cells and warrants additional studies that may assist in improved early diagnosis and treatment of shammah induced oral cancer.Shankargouda PatilMohd Younis BhatJayshree AdvaniSonali V. MohanNiraj BabuKeshava K. DattaTejaswini SubbannayyaPavithra RajagopalanFirdous A. BhatNezar Al-hebshiDavid SidranskyHarsha GowdaAditi ChatterjeeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shankargouda Patil Mohd Younis Bhat Jayshree Advani Sonali V. Mohan Niraj Babu Keshava K. Datta Tejaswini Subbannayya Pavithra Rajagopalan Firdous A. Bhat Nezar Al-hebshi David Sidransky Harsha Gowda Aditi Chatterjee Proteomic and phosphoproteomic profiling of shammah induced signaling in oral keratinocytes
description	Abstract Shammah is a smokeless tobacco product often mixed with lime, ash, black pepper and flavorings. Exposure to shammah has been linked with dental diseases and oral squamous cell carcinoma. There is limited literature on the prevalence of shammah and its role in pathobiology of oral cancer. In this study, we developed a cellular model to understand the effect of chronic shammah exposure on oral keratinocytes. Chronic exposure to shammah resulted in increased proliferation and invasiveness of non-transformed oral keratinocytes. Quantitative proteomics of shammah treated cells compared to untreated cells led to quantification of 4712 proteins of which 402 were found to be significantly altered. In addition, phosphoproteomics analysis of shammah treated cells compared to untreated revealed hyperphosphorylation of 36 proteins and hypophosphorylation of 83 proteins (twofold, p-value ≤ 0.05). Bioinformatics analysis of significantly altered proteins showed enrichment of proteins involved in extracellular matrix interactions, necroptosis and peroxisome mediated fatty acid oxidation. Kinase-Substrate Enrichment Analysis showed significant increase in activity of kinases such as ROCK1, RAF1, PRKCE and HIPK2 in shammah treated cells. These results provide better understanding of how shammah transforms non-neoplastic cells and warrants additional studies that may assist in improved early diagnosis and treatment of shammah induced oral cancer.
format	article
author	Shankargouda Patil Mohd Younis Bhat Jayshree Advani Sonali V. Mohan Niraj Babu Keshava K. Datta Tejaswini Subbannayya Pavithra Rajagopalan Firdous A. Bhat Nezar Al-hebshi David Sidransky Harsha Gowda Aditi Chatterjee
author_facet	Shankargouda Patil Mohd Younis Bhat Jayshree Advani Sonali V. Mohan Niraj Babu Keshava K. Datta Tejaswini Subbannayya Pavithra Rajagopalan Firdous A. Bhat Nezar Al-hebshi David Sidransky Harsha Gowda Aditi Chatterjee
author_sort	Shankargouda Patil
title	Proteomic and phosphoproteomic profiling of shammah induced signaling in oral keratinocytes
title_short	Proteomic and phosphoproteomic profiling of shammah induced signaling in oral keratinocytes
title_full	Proteomic and phosphoproteomic profiling of shammah induced signaling in oral keratinocytes
title_fullStr	Proteomic and phosphoproteomic profiling of shammah induced signaling in oral keratinocytes
title_full_unstemmed	Proteomic and phosphoproteomic profiling of shammah induced signaling in oral keratinocytes
title_sort	proteomic and phosphoproteomic profiling of shammah induced signaling in oral keratinocytes
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8f0e8442e1844fe5a525d733299b371e
work_keys_str_mv	AT shankargoudapatil proteomicandphosphoproteomicprofilingofshammahinducedsignalinginoralkeratinocytes AT mohdyounisbhat proteomicandphosphoproteomicprofilingofshammahinducedsignalinginoralkeratinocytes AT jayshreeadvani proteomicandphosphoproteomicprofilingofshammahinducedsignalinginoralkeratinocytes AT sonalivmohan proteomicandphosphoproteomicprofilingofshammahinducedsignalinginoralkeratinocytes AT nirajbabu proteomicandphosphoproteomicprofilingofshammahinducedsignalinginoralkeratinocytes AT keshavakdatta proteomicandphosphoproteomicprofilingofshammahinducedsignalinginoralkeratinocytes AT tejaswinisubbannayya proteomicandphosphoproteomicprofilingofshammahinducedsignalinginoralkeratinocytes AT pavithrarajagopalan proteomicandphosphoproteomicprofilingofshammahinducedsignalinginoralkeratinocytes AT firdousabhat proteomicandphosphoproteomicprofilingofshammahinducedsignalinginoralkeratinocytes AT nezaralhebshi proteomicandphosphoproteomicprofilingofshammahinducedsignalinginoralkeratinocytes AT davidsidransky proteomicandphosphoproteomicprofilingofshammahinducedsignalinginoralkeratinocytes AT harshagowda proteomicandphosphoproteomicprofilingofshammahinducedsignalinginoralkeratinocytes AT aditichatterjee proteomicandphosphoproteomicprofilingofshammahinducedsignalinginoralkeratinocytes
_version_	1718381271994859520

Proteomic and phosphoproteomic profiling of shammah induced signaling in oral keratinocytes

Ejemplares similares