The vascular targeted citrus FLOWERING LOCUS T3 gene promotes non-inductive early flowering in transgenic Carrizo rootstocks and grafted juvenile scions

The vascular targeted citrus FLOWERING LOCUS T3 gene promotes non-inductive early flowering in transgenic Carrizo rootstocks and grafted juvenile scions

Abstract Shortening the juvenile stage in citrus and inducing early flowering has been the focus of several citrus genetic improvement programs. FLOWERING LOCUS T (FT) is a small phloem-translocated protein that regulates precocious flowering. In this study, two populations of transgenic Carrizo cit...

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Autores principales: Juliana M. Soares, Kyle C. Weber, Wenming Qiu, Daniel Stanton, Lamiaa M. Mahmoud, Hao Wu, Patrick Huyck, Janice Zale, Kawther Al Jasim, Jude W. Grosser, Manjul Dutt
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Publicado: Nature Portfolio 2020
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Acceso en línea:https://doaj.org/article/8f121e382ff049bab1daf03031df9ea2
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spelling oai:doaj.org-article:8f121e382ff049bab1daf03031df9ea22021-12-02T12:33:05ZThe vascular targeted citrus FLOWERING LOCUS T3 gene promotes non-inductive early flowering in transgenic Carrizo rootstocks and grafted juvenile scions10.1038/s41598-020-78417-92045-2322https://doaj.org/article/8f121e382ff049bab1daf03031df9ea22020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78417-9https://doaj.org/toc/2045-2322Abstract Shortening the juvenile stage in citrus and inducing early flowering has been the focus of several citrus genetic improvement programs. FLOWERING LOCUS T (FT) is a small phloem-translocated protein that regulates precocious flowering. In this study, two populations of transgenic Carrizo citrange rootstocks expressing either Citrus clementina FT1 or FT3 genes under the control of the Arabidopsis thaliana phloem specific SUCROSE SYNTHASE 2 (AtSUC2) promoter were developed. The transgenic plants were morphologically similar to the non-transgenic controls (non-transgenic Carrizo citrange), however, only AtSUC2-CcFT3 was capable of inducing precocious flowers. The transgenic lines produced flowers 16 months after transformation and flower buds appeared 30–40 days on juvenile immature scions grafted onto transgenic rootstock. Gene expression analysis revealed that the expression of SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1) and APETALA1 (AP1) were enhanced in the transgenics. Transcriptome profiling of a selected transgenic line showed the induction of genes in different groups including: genes from the flowering induction pathway, APETALA2/ETHYLENE RESPONSE FACTOR (AP2/ERF) family genes, and jasmonic acid (JA) pathway genes. Altogether, our results suggested that ectopic expression of CcFT3 in phloem tissues of Carrizo citrange triggered the expression of several genes to mediate early flowering.Juliana M. SoaresKyle C. WeberWenming QiuDaniel StantonLamiaa M. MahmoudHao WuPatrick HuyckJanice ZaleKawther Al JasimJude W. GrosserManjul DuttNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-18 (2020)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Juliana M. Soares
Kyle C. Weber
Wenming Qiu
Daniel Stanton
Lamiaa M. Mahmoud
Hao Wu
Patrick Huyck
Janice Zale
Kawther Al Jasim
Jude W. Grosser
Manjul Dutt
The vascular targeted citrus FLOWERING LOCUS T3 gene promotes non-inductive early flowering in transgenic Carrizo rootstocks and grafted juvenile scions
description Abstract Shortening the juvenile stage in citrus and inducing early flowering has been the focus of several citrus genetic improvement programs. FLOWERING LOCUS T (FT) is a small phloem-translocated protein that regulates precocious flowering. In this study, two populations of transgenic Carrizo citrange rootstocks expressing either Citrus clementina FT1 or FT3 genes under the control of the Arabidopsis thaliana phloem specific SUCROSE SYNTHASE 2 (AtSUC2) promoter were developed. The transgenic plants were morphologically similar to the non-transgenic controls (non-transgenic Carrizo citrange), however, only AtSUC2-CcFT3 was capable of inducing precocious flowers. The transgenic lines produced flowers 16 months after transformation and flower buds appeared 30–40 days on juvenile immature scions grafted onto transgenic rootstock. Gene expression analysis revealed that the expression of SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1) and APETALA1 (AP1) were enhanced in the transgenics. Transcriptome profiling of a selected transgenic line showed the induction of genes in different groups including: genes from the flowering induction pathway, APETALA2/ETHYLENE RESPONSE FACTOR (AP2/ERF) family genes, and jasmonic acid (JA) pathway genes. Altogether, our results suggested that ectopic expression of CcFT3 in phloem tissues of Carrizo citrange triggered the expression of several genes to mediate early flowering.
format article
author Juliana M. Soares
Kyle C. Weber
Wenming Qiu
Daniel Stanton
Lamiaa M. Mahmoud
Hao Wu
Patrick Huyck
Janice Zale
Kawther Al Jasim
Jude W. Grosser
Manjul Dutt
author_facet Juliana M. Soares
Kyle C. Weber
Wenming Qiu
Daniel Stanton
Lamiaa M. Mahmoud
Hao Wu
Patrick Huyck
Janice Zale
Kawther Al Jasim
Jude W. Grosser
Manjul Dutt
author_sort Juliana M. Soares
title The vascular targeted citrus FLOWERING LOCUS T3 gene promotes non-inductive early flowering in transgenic Carrizo rootstocks and grafted juvenile scions
title_short The vascular targeted citrus FLOWERING LOCUS T3 gene promotes non-inductive early flowering in transgenic Carrizo rootstocks and grafted juvenile scions
title_full The vascular targeted citrus FLOWERING LOCUS T3 gene promotes non-inductive early flowering in transgenic Carrizo rootstocks and grafted juvenile scions
title_fullStr The vascular targeted citrus FLOWERING LOCUS T3 gene promotes non-inductive early flowering in transgenic Carrizo rootstocks and grafted juvenile scions
title_full_unstemmed The vascular targeted citrus FLOWERING LOCUS T3 gene promotes non-inductive early flowering in transgenic Carrizo rootstocks and grafted juvenile scions
title_sort vascular targeted citrus flowering locus t3 gene promotes non-inductive early flowering in transgenic carrizo rootstocks and grafted juvenile scions
publisher Nature Portfolio
publishDate 2020
url https://doaj.org/article/8f121e382ff049bab1daf03031df9ea2
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