Transcriptomic response of durum wheat to nitrogen starvation

Abstract Nitrogen (N) is a key macronutrient representing a limiting factor for plant growth and development and affects productivity in wheat. In this study, durum wheat response to N chronic starvation during grain filling was investigated through a transcriptomic approach in roots, leaves/stems,...

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Autores principales:	Pasquale L. Curci, Riccardo Aiese Cigliano, Diana L. Zuluaga, Michela Janni, Walter Sanseverino, Gabriella Sonnante
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8f178b35e4cb4052915ddcf52d4e0a64
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spelling	oai:doaj.org-article:8f178b35e4cb4052915ddcf52d4e0a642021-12-02T16:06:16ZTranscriptomic response of durum wheat to nitrogen starvation10.1038/s41598-017-01377-02045-2322https://doaj.org/article/8f178b35e4cb4052915ddcf52d4e0a642017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01377-0https://doaj.org/toc/2045-2322Abstract Nitrogen (N) is a key macronutrient representing a limiting factor for plant growth and development and affects productivity in wheat. In this study, durum wheat response to N chronic starvation during grain filling was investigated through a transcriptomic approach in roots, leaves/stems, flag leaf and spikes of cv. Svevo. Nitrogen stress negatively influenced plant height, tillering, flag leaf area, spike and seed traits, and total N content. RNA-seq data revealed 4,626 differentially expressed genes (DEGs). Most transcriptomic changes were observed in roots, with 3,270 DEGs, while 963 were found in leaves/stems, 470 in flag leaf, and 355 in spike tissues. A total of 799 gene ontology (GO) terms were identified, 180 and 619 among the upregulated and downregulated genes, respectively. Among the most addressed GO categories, N compound metabolism, carbon metabolism, and photosynthesis were mostly represented. Interesting DEGs, such as N transporters, genes involved in N assimilation, along with transcription factors, protein kinases and other genes related to stress were highlighted. These results provide valuable information about the transcriptomic response to chronic N stress in durum wheat, which could be useful for future improvement of N use efficiency.Pasquale L. CurciRiccardo Aiese CiglianoDiana L. ZuluagaMichela JanniWalter SanseverinoGabriella SonnanteNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pasquale L. Curci Riccardo Aiese Cigliano Diana L. Zuluaga Michela Janni Walter Sanseverino Gabriella Sonnante Transcriptomic response of durum wheat to nitrogen starvation
description	Abstract Nitrogen (N) is a key macronutrient representing a limiting factor for plant growth and development and affects productivity in wheat. In this study, durum wheat response to N chronic starvation during grain filling was investigated through a transcriptomic approach in roots, leaves/stems, flag leaf and spikes of cv. Svevo. Nitrogen stress negatively influenced plant height, tillering, flag leaf area, spike and seed traits, and total N content. RNA-seq data revealed 4,626 differentially expressed genes (DEGs). Most transcriptomic changes were observed in roots, with 3,270 DEGs, while 963 were found in leaves/stems, 470 in flag leaf, and 355 in spike tissues. A total of 799 gene ontology (GO) terms were identified, 180 and 619 among the upregulated and downregulated genes, respectively. Among the most addressed GO categories, N compound metabolism, carbon metabolism, and photosynthesis were mostly represented. Interesting DEGs, such as N transporters, genes involved in N assimilation, along with transcription factors, protein kinases and other genes related to stress were highlighted. These results provide valuable information about the transcriptomic response to chronic N stress in durum wheat, which could be useful for future improvement of N use efficiency.
format	article
author	Pasquale L. Curci Riccardo Aiese Cigliano Diana L. Zuluaga Michela Janni Walter Sanseverino Gabriella Sonnante
author_facet	Pasquale L. Curci Riccardo Aiese Cigliano Diana L. Zuluaga Michela Janni Walter Sanseverino Gabriella Sonnante
author_sort	Pasquale L. Curci
title	Transcriptomic response of durum wheat to nitrogen starvation
title_short	Transcriptomic response of durum wheat to nitrogen starvation
title_full	Transcriptomic response of durum wheat to nitrogen starvation
title_fullStr	Transcriptomic response of durum wheat to nitrogen starvation
title_full_unstemmed	Transcriptomic response of durum wheat to nitrogen starvation
title_sort	transcriptomic response of durum wheat to nitrogen starvation
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8f178b35e4cb4052915ddcf52d4e0a64
work_keys_str_mv	AT pasqualelcurci transcriptomicresponseofdurumwheattonitrogenstarvation AT riccardoaiesecigliano transcriptomicresponseofdurumwheattonitrogenstarvation AT dianalzuluaga transcriptomicresponseofdurumwheattonitrogenstarvation AT michelajanni transcriptomicresponseofdurumwheattonitrogenstarvation AT waltersanseverino transcriptomicresponseofdurumwheattonitrogenstarvation AT gabriellasonnante transcriptomicresponseofdurumwheattonitrogenstarvation
_version_	1718385037633650688

Transcriptomic response of durum wheat to nitrogen starvation

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