Biological and antimicrobial properties of the association Ambroxol and a water-soluble viscous liquid as a vehicle for a tricalcium silicate-based sealer

Abstract This study aimed to investigate the antimicrobial and biological properties of Ambroxol associated with glycerin (GLI), propylene glycol (PG), and polyethylene glycol (PEG) as a possible vehicle for an experimental tricalcium silicate sealer, with the intention of developing a new biomateri...

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Autores principales:	Índia Olinta de Azevedo Queiroz, Thiago Machado, Camila Carneiro Alves, Victor Gustavo Balera Brito, Bruno Carvalho de Vasconcelos, João Eduardo Gomes-Filho, Edilson Ervolino, Sandra Helena Penha de Oliveira, Marco Antonio Hungaro Duarte
Formato:	article
Lenguaje:	EN
Publicado:	Springer 2021
Materias:	Materials of engineering and construction. Mechanics of materials TA401-492 Medical technology R855-855.5
Acceso en línea:	https://doaj.org/article/8f29aefa681b4871bd87faa1bb442e60
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spelling	oai:doaj.org-article:8f29aefa681b4871bd87faa1bb442e602021-11-28T12:26:53ZBiological and antimicrobial properties of the association Ambroxol and a water-soluble viscous liquid as a vehicle for a tricalcium silicate-based sealer10.1007/s10856-021-06604-90957-45301573-4838https://doaj.org/article/8f29aefa681b4871bd87faa1bb442e602021-11-01T00:00:00Zhttps://doi.org/10.1007/s10856-021-06604-9https://doaj.org/toc/0957-4530https://doaj.org/toc/1573-4838Abstract This study aimed to investigate the antimicrobial and biological properties of Ambroxol associated with glycerin (GLI), propylene glycol (PG), and polyethylene glycol (PEG) as a possible vehicle for an experimental tricalcium silicate sealer, with the intention of developing a new biomaterial. Mouse undifferentiated dental pulp cells (OD-21) were cultured, and the effects of different association on cell proliferation and inflammatory cytokine production were investigated. Antimicrobial adhesion of Enterococcus faecalis to setting sealers at 2 h was evaluated. Polyethylene tubes containing experimental sealers and empty tubes were implanted into dorsal connective tissues of 12 male 3- to 4-months-old Wistar rats (250–280 g). After 7 and 30 days, the tubes were removed and processed for histological and immunohistochemical analyses. ANOVA followed by Bonferroni correction and ANOVA followed by Tukey test was used for parametric data and Kruskal–Wallis followed by Dunn for nonparametric (p < 0.05). Cell proliferation was dose-dependent, since all association were cytotoxic at higher concentrations; however, Ambroxol–PEG showed significantly higher cytotoxicity than other association (p < 0.05). In addition, irrespective of the association, no cytokine production was observed in vitro. Ambroxol–GLI reduced bacterial viability, whereas Ambroxol–PEG increased (p < 0.05). Histological examination showed no significant difference in the inflammatory response (p > 0.05) and mineralization ability in all association. Additionally, IL-1β and TNF-α were upregulated on Ambroxol–PEG in relation to Control at 07 days (p < 0.05). Ambroxol–GLI was the best vehicle for experimental tricalcium silicate sealer, as it promoted an increase in antimicrobial activity without altering the inflammatory response or mineralization ability.Índia Olinta de Azevedo QueirozThiago MachadoCamila Carneiro AlvesVictor Gustavo Balera BritoBruno Carvalho de VasconcelosJoão Eduardo Gomes-FilhoEdilson ErvolinoSandra Helena Penha de OliveiraMarco Antonio Hungaro DuarteSpringerarticleMaterials of engineering and construction. Mechanics of materialsTA401-492Medical technologyR855-855.5ENJournal of Materials Science: Materials in Medicine, Vol 32, Iss 12, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Materials of engineering and construction. Mechanics of materials TA401-492 Medical technology R855-855.5
spellingShingle	Materials of engineering and construction. Mechanics of materials TA401-492 Medical technology R855-855.5 Índia Olinta de Azevedo Queiroz Thiago Machado Camila Carneiro Alves Victor Gustavo Balera Brito Bruno Carvalho de Vasconcelos João Eduardo Gomes-Filho Edilson Ervolino Sandra Helena Penha de Oliveira Marco Antonio Hungaro Duarte Biological and antimicrobial properties of the association Ambroxol and a water-soluble viscous liquid as a vehicle for a tricalcium silicate-based sealer
description	Abstract This study aimed to investigate the antimicrobial and biological properties of Ambroxol associated with glycerin (GLI), propylene glycol (PG), and polyethylene glycol (PEG) as a possible vehicle for an experimental tricalcium silicate sealer, with the intention of developing a new biomaterial. Mouse undifferentiated dental pulp cells (OD-21) were cultured, and the effects of different association on cell proliferation and inflammatory cytokine production were investigated. Antimicrobial adhesion of Enterococcus faecalis to setting sealers at 2 h was evaluated. Polyethylene tubes containing experimental sealers and empty tubes were implanted into dorsal connective tissues of 12 male 3- to 4-months-old Wistar rats (250–280 g). After 7 and 30 days, the tubes were removed and processed for histological and immunohistochemical analyses. ANOVA followed by Bonferroni correction and ANOVA followed by Tukey test was used for parametric data and Kruskal–Wallis followed by Dunn for nonparametric (p < 0.05). Cell proliferation was dose-dependent, since all association were cytotoxic at higher concentrations; however, Ambroxol–PEG showed significantly higher cytotoxicity than other association (p < 0.05). In addition, irrespective of the association, no cytokine production was observed in vitro. Ambroxol–GLI reduced bacterial viability, whereas Ambroxol–PEG increased (p < 0.05). Histological examination showed no significant difference in the inflammatory response (p > 0.05) and mineralization ability in all association. Additionally, IL-1β and TNF-α were upregulated on Ambroxol–PEG in relation to Control at 07 days (p < 0.05). Ambroxol–GLI was the best vehicle for experimental tricalcium silicate sealer, as it promoted an increase in antimicrobial activity without altering the inflammatory response or mineralization ability.
format	article
author	Índia Olinta de Azevedo Queiroz Thiago Machado Camila Carneiro Alves Victor Gustavo Balera Brito Bruno Carvalho de Vasconcelos João Eduardo Gomes-Filho Edilson Ervolino Sandra Helena Penha de Oliveira Marco Antonio Hungaro Duarte
author_facet	Índia Olinta de Azevedo Queiroz Thiago Machado Camila Carneiro Alves Victor Gustavo Balera Brito Bruno Carvalho de Vasconcelos João Eduardo Gomes-Filho Edilson Ervolino Sandra Helena Penha de Oliveira Marco Antonio Hungaro Duarte
author_sort	Índia Olinta de Azevedo Queiroz
title	Biological and antimicrobial properties of the association Ambroxol and a water-soluble viscous liquid as a vehicle for a tricalcium silicate-based sealer
title_short	Biological and antimicrobial properties of the association Ambroxol and a water-soluble viscous liquid as a vehicle for a tricalcium silicate-based sealer
title_full	Biological and antimicrobial properties of the association Ambroxol and a water-soluble viscous liquid as a vehicle for a tricalcium silicate-based sealer
title_fullStr	Biological and antimicrobial properties of the association Ambroxol and a water-soluble viscous liquid as a vehicle for a tricalcium silicate-based sealer
title_full_unstemmed	Biological and antimicrobial properties of the association Ambroxol and a water-soluble viscous liquid as a vehicle for a tricalcium silicate-based sealer
title_sort	biological and antimicrobial properties of the association ambroxol and a water-soluble viscous liquid as a vehicle for a tricalcium silicate-based sealer
publisher	Springer
publishDate	2021
url	https://doaj.org/article/8f29aefa681b4871bd87faa1bb442e60
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Biological and antimicrobial properties of the association Ambroxol and a water-soluble viscous liquid as a vehicle for a tricalcium silicate-based sealer

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