Identification of target genes regulated by the Drosophila histone methyltransferase Eggless reveals a role of Decapentaplegic in apoptotic signaling

Abstract Epigenetic gene regulation is essential for developmental processes. Eggless (Egg), the Drosophila orthologue of the mammalian histone methyltransferase, SETDB1, is known to be involved in the survival and differentiation of germline stem cells and piRNA cluster transcription during Drosoph...

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Autores principales:	Igojo Kang, Yourim Choi, Sueun Jung, Jae Yun Lim, Dooyoung Lee, Sumeet Gupta, Woongjoon Moon, Chanseok Shin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8f3ab14e92274215968513f9f7d8bd6c
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spelling	oai:doaj.org-article:8f3ab14e92274215968513f9f7d8bd6c2021-12-02T11:40:54ZIdentification of target genes regulated by the Drosophila histone methyltransferase Eggless reveals a role of Decapentaplegic in apoptotic signaling10.1038/s41598-018-25483-92045-2322https://doaj.org/article/8f3ab14e92274215968513f9f7d8bd6c2018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25483-9https://doaj.org/toc/2045-2322Abstract Epigenetic gene regulation is essential for developmental processes. Eggless (Egg), the Drosophila orthologue of the mammalian histone methyltransferase, SETDB1, is known to be involved in the survival and differentiation of germline stem cells and piRNA cluster transcription during Drosophila oogenesis; however the detailed mechanisms remain to be determined. Here, using high-throughput RNA sequencing, we investigated target genes regulated by Egg in an unbiased manner. We show that Egg plays diverse roles in particular piRNA pathway gene expression, some long non-coding RNA expression, apoptosis-related gene regulation, and Decapentaplegic (Dpp) signaling during Drosophila oogenesis. Furthermore, using genetic and cell biological approaches, we demonstrate that ectopic upregulation of dpp caused by loss of Egg in the germarium can trigger apoptotic cell death through activation of two pro-apoptotic genes, reaper and head involution defective. We propose a model in which Egg regulates germ cell differentiation and apoptosis through canonical and noncanonical Dpp pathways in Drosophila oogenesis.Igojo KangYourim ChoiSueun JungJae Yun LimDooyoung LeeSumeet GuptaWoongjoon MoonChanseok ShinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Igojo Kang Yourim Choi Sueun Jung Jae Yun Lim Dooyoung Lee Sumeet Gupta Woongjoon Moon Chanseok Shin Identification of target genes regulated by the Drosophila histone methyltransferase Eggless reveals a role of Decapentaplegic in apoptotic signaling
description	Abstract Epigenetic gene regulation is essential for developmental processes. Eggless (Egg), the Drosophila orthologue of the mammalian histone methyltransferase, SETDB1, is known to be involved in the survival and differentiation of germline stem cells and piRNA cluster transcription during Drosophila oogenesis; however the detailed mechanisms remain to be determined. Here, using high-throughput RNA sequencing, we investigated target genes regulated by Egg in an unbiased manner. We show that Egg plays diverse roles in particular piRNA pathway gene expression, some long non-coding RNA expression, apoptosis-related gene regulation, and Decapentaplegic (Dpp) signaling during Drosophila oogenesis. Furthermore, using genetic and cell biological approaches, we demonstrate that ectopic upregulation of dpp caused by loss of Egg in the germarium can trigger apoptotic cell death through activation of two pro-apoptotic genes, reaper and head involution defective. We propose a model in which Egg regulates germ cell differentiation and apoptosis through canonical and noncanonical Dpp pathways in Drosophila oogenesis.
format	article
author	Igojo Kang Yourim Choi Sueun Jung Jae Yun Lim Dooyoung Lee Sumeet Gupta Woongjoon Moon Chanseok Shin
author_facet	Igojo Kang Yourim Choi Sueun Jung Jae Yun Lim Dooyoung Lee Sumeet Gupta Woongjoon Moon Chanseok Shin
author_sort	Igojo Kang
title	Identification of target genes regulated by the Drosophila histone methyltransferase Eggless reveals a role of Decapentaplegic in apoptotic signaling
title_short	Identification of target genes regulated by the Drosophila histone methyltransferase Eggless reveals a role of Decapentaplegic in apoptotic signaling
title_full	Identification of target genes regulated by the Drosophila histone methyltransferase Eggless reveals a role of Decapentaplegic in apoptotic signaling
title_fullStr	Identification of target genes regulated by the Drosophila histone methyltransferase Eggless reveals a role of Decapentaplegic in apoptotic signaling
title_full_unstemmed	Identification of target genes regulated by the Drosophila histone methyltransferase Eggless reveals a role of Decapentaplegic in apoptotic signaling
title_sort	identification of target genes regulated by the drosophila histone methyltransferase eggless reveals a role of decapentaplegic in apoptotic signaling
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/8f3ab14e92274215968513f9f7d8bd6c
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Identification of target genes regulated by the Drosophila histone methyltransferase Eggless reveals a role of Decapentaplegic in apoptotic signaling

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