Genomic survey of the non-cultivatable opportunistic human pathogen, Enterocytozoon bieneusi.
Enterocytozoon bieneusi is the most common microsporidian associated with human disease, particularly in the immunocompromised population. In the setting of HIV infection, it is associated with diarrhea and wasting syndrome. Like all microsporidia, E. bieneusi is an obligate, intracellular parasite,...
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Public Library of Science (PLoS)
2009
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oai:doaj.org-article:8f3b63a125a644e789ba24722f68d85e2021-11-25T05:47:21ZGenomic survey of the non-cultivatable opportunistic human pathogen, Enterocytozoon bieneusi.1553-73661553-737410.1371/journal.ppat.1000261https://doaj.org/article/8f3b63a125a644e789ba24722f68d85e2009-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19132089/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Enterocytozoon bieneusi is the most common microsporidian associated with human disease, particularly in the immunocompromised population. In the setting of HIV infection, it is associated with diarrhea and wasting syndrome. Like all microsporidia, E. bieneusi is an obligate, intracellular parasite, but unlike others, it is in direct contact with the host cell cytoplasm. Studies of E. bieneusi have been greatly limited due to the absence of genomic data and lack of a robust cultivation system. Here, we present the first large-scale genomic dataset for E. bieneusi. Approximately 3.86 Mb of unique sequence was generated by paired end Sanger sequencing, representing about 64% of the estimated 6 Mb genome. A total of 3,804 genes were identified in E. bieneusi, of which 1,702 encode proteins with assigned functions. Of these, 653 are homologs of Encephalitozoon cuniculi proteins. Only one E. bieneusi protein with assigned function had no E. cuniculi homolog. The shared proteins were, in general, evenly distributed among the functional categories, with the exception of a dearth of genes encoding proteins associated with pathways for fatty acid and core carbon metabolism. Short intergenic regions, high gene density, and shortened protein-coding sequences were observed in the E. bieneusi genome, all traits consistent with genomic compaction. Our findings suggest that E. bieneusi is a likely model for extreme genome reduction and host dependence.Donna E AkiyoshiHilary G MorrisonShi LeiXiaochuan FengQuanshun ZhangNicolas CorradiHarriet MayanjaJames K TumwinePatrick J KeelingLouis M WeissSaul TziporiPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 5, Iss 1, p e1000261 (2009) |
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DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 |
| spellingShingle |
Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Donna E Akiyoshi Hilary G Morrison Shi Lei Xiaochuan Feng Quanshun Zhang Nicolas Corradi Harriet Mayanja James K Tumwine Patrick J Keeling Louis M Weiss Saul Tzipori Genomic survey of the non-cultivatable opportunistic human pathogen, Enterocytozoon bieneusi. |
| description |
Enterocytozoon bieneusi is the most common microsporidian associated with human disease, particularly in the immunocompromised population. In the setting of HIV infection, it is associated with diarrhea and wasting syndrome. Like all microsporidia, E. bieneusi is an obligate, intracellular parasite, but unlike others, it is in direct contact with the host cell cytoplasm. Studies of E. bieneusi have been greatly limited due to the absence of genomic data and lack of a robust cultivation system. Here, we present the first large-scale genomic dataset for E. bieneusi. Approximately 3.86 Mb of unique sequence was generated by paired end Sanger sequencing, representing about 64% of the estimated 6 Mb genome. A total of 3,804 genes were identified in E. bieneusi, of which 1,702 encode proteins with assigned functions. Of these, 653 are homologs of Encephalitozoon cuniculi proteins. Only one E. bieneusi protein with assigned function had no E. cuniculi homolog. The shared proteins were, in general, evenly distributed among the functional categories, with the exception of a dearth of genes encoding proteins associated with pathways for fatty acid and core carbon metabolism. Short intergenic regions, high gene density, and shortened protein-coding sequences were observed in the E. bieneusi genome, all traits consistent with genomic compaction. Our findings suggest that E. bieneusi is a likely model for extreme genome reduction and host dependence. |
| format |
article |
| author |
Donna E Akiyoshi Hilary G Morrison Shi Lei Xiaochuan Feng Quanshun Zhang Nicolas Corradi Harriet Mayanja James K Tumwine Patrick J Keeling Louis M Weiss Saul Tzipori |
| author_facet |
Donna E Akiyoshi Hilary G Morrison Shi Lei Xiaochuan Feng Quanshun Zhang Nicolas Corradi Harriet Mayanja James K Tumwine Patrick J Keeling Louis M Weiss Saul Tzipori |
| author_sort |
Donna E Akiyoshi |
| title |
Genomic survey of the non-cultivatable opportunistic human pathogen, Enterocytozoon bieneusi. |
| title_short |
Genomic survey of the non-cultivatable opportunistic human pathogen, Enterocytozoon bieneusi. |
| title_full |
Genomic survey of the non-cultivatable opportunistic human pathogen, Enterocytozoon bieneusi. |
| title_fullStr |
Genomic survey of the non-cultivatable opportunistic human pathogen, Enterocytozoon bieneusi. |
| title_full_unstemmed |
Genomic survey of the non-cultivatable opportunistic human pathogen, Enterocytozoon bieneusi. |
| title_sort |
genomic survey of the non-cultivatable opportunistic human pathogen, enterocytozoon bieneusi. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2009 |
| url |
https://doaj.org/article/8f3b63a125a644e789ba24722f68d85e |
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| _version_ |
1718414469835522048 |