Optimization of polycaprolactone - based nanofiber matrices for the cultivation of corneal endothelial cells

Abstract Posterior lamellar transplantation of the eye’ s cornea (DSAEK, DMEK) currently is the gold standard for treating patients with corneal endothelial cell and back surface pathologies resulting in functional impairment. An artificial biomimetic graft carrying human corneal endothelium could m...

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Autores principales:	Marcus Himmler, Fabian Garreis, Friedrich Paulsen, Dirk W. Schubert, Thomas A. Fuchsluger
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8f3eea5ac78040f1a0896901f3a44563
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spelling	oai:doaj.org-article:8f3eea5ac78040f1a0896901f3a445632021-12-02T15:15:04ZOptimization of polycaprolactone - based nanofiber matrices for the cultivation of corneal endothelial cells10.1038/s41598-021-98426-62045-2322https://doaj.org/article/8f3eea5ac78040f1a0896901f3a445632021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98426-6https://doaj.org/toc/2045-2322Abstract Posterior lamellar transplantation of the eye’ s cornea (DSAEK, DMEK) currently is the gold standard for treating patients with corneal endothelial cell and back surface pathologies resulting in functional impairment. An artificial biomimetic graft carrying human corneal endothelium could minimize the dependency on human donor corneas giving access to this vision-restoring surgery to large numbers of patients, thus reducing current long waiting lists. In this study, four groups of electrospun nanofibrous scaffolds were compared: polycaprolactone (PCL), PCL/collagen, PCL/gelatin and PCL/chitosan. Each of the scaffolds were tissue-engineered with human corneal endothelial cells (HCEC-B4G12) and analyzed with regard to their potential application as artificial posterior lamellar grafts. Staining with ZO-1 and Na+/K+-ATPase antibodies revealed intact cell functionalities. It could be shown, that blending leads to decreasing contact angle, whereby a heterogeneous blend morphology could be revealed. Scaffold cytocompatibility could be confirmed for all groups via live/dead staining, whereby a significant higher cell viability could be observed for the collagen and gelatine blended matrices with 97 ± 3% and 98 ± 2% living cells respectively. TEM images show the superficial anchoring of the HCECs onto the scaffolds. This work emphasizes the benefit of blended PCL nanofibrous scaffolds for corneal endothelial keratoplasty.Marcus HimmlerFabian GarreisFriedrich PaulsenDirk W. SchubertThomas A. FuchslugerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marcus Himmler Fabian Garreis Friedrich Paulsen Dirk W. Schubert Thomas A. Fuchsluger Optimization of polycaprolactone - based nanofiber matrices for the cultivation of corneal endothelial cells
description	Abstract Posterior lamellar transplantation of the eye’ s cornea (DSAEK, DMEK) currently is the gold standard for treating patients with corneal endothelial cell and back surface pathologies resulting in functional impairment. An artificial biomimetic graft carrying human corneal endothelium could minimize the dependency on human donor corneas giving access to this vision-restoring surgery to large numbers of patients, thus reducing current long waiting lists. In this study, four groups of electrospun nanofibrous scaffolds were compared: polycaprolactone (PCL), PCL/collagen, PCL/gelatin and PCL/chitosan. Each of the scaffolds were tissue-engineered with human corneal endothelial cells (HCEC-B4G12) and analyzed with regard to their potential application as artificial posterior lamellar grafts. Staining with ZO-1 and Na+/K+-ATPase antibodies revealed intact cell functionalities. It could be shown, that blending leads to decreasing contact angle, whereby a heterogeneous blend morphology could be revealed. Scaffold cytocompatibility could be confirmed for all groups via live/dead staining, whereby a significant higher cell viability could be observed for the collagen and gelatine blended matrices with 97 ± 3% and 98 ± 2% living cells respectively. TEM images show the superficial anchoring of the HCECs onto the scaffolds. This work emphasizes the benefit of blended PCL nanofibrous scaffolds for corneal endothelial keratoplasty.
format	article
author	Marcus Himmler Fabian Garreis Friedrich Paulsen Dirk W. Schubert Thomas A. Fuchsluger
author_facet	Marcus Himmler Fabian Garreis Friedrich Paulsen Dirk W. Schubert Thomas A. Fuchsluger
author_sort	Marcus Himmler
title	Optimization of polycaprolactone - based nanofiber matrices for the cultivation of corneal endothelial cells
title_short	Optimization of polycaprolactone - based nanofiber matrices for the cultivation of corneal endothelial cells
title_full	Optimization of polycaprolactone - based nanofiber matrices for the cultivation of corneal endothelial cells
title_fullStr	Optimization of polycaprolactone - based nanofiber matrices for the cultivation of corneal endothelial cells
title_full_unstemmed	Optimization of polycaprolactone - based nanofiber matrices for the cultivation of corneal endothelial cells
title_sort	optimization of polycaprolactone - based nanofiber matrices for the cultivation of corneal endothelial cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8f3eea5ac78040f1a0896901f3a44563
work_keys_str_mv	AT marcushimmler optimizationofpolycaprolactonebasednanofibermatricesforthecultivationofcornealendothelialcells AT fabiangarreis optimizationofpolycaprolactonebasednanofibermatricesforthecultivationofcornealendothelialcells AT friedrichpaulsen optimizationofpolycaprolactonebasednanofibermatricesforthecultivationofcornealendothelialcells AT dirkwschubert optimizationofpolycaprolactonebasednanofibermatricesforthecultivationofcornealendothelialcells AT thomasafuchsluger optimizationofpolycaprolactonebasednanofibermatricesforthecultivationofcornealendothelialcells
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Optimization of polycaprolactone - based nanofiber matrices for the cultivation of corneal endothelial cells

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