Modulatory effects of Benjakul extract on rat hepatic cytochrome P450 enzymes

Benjakul, a traditional Thai formulation, has been used as a carminative and adaptogenic drug. It consists of five plants, Piper chaba Hunter, Piper sarmentosum Roxb., Piper interruptum Opiz., Plumbago indica Linn., and Zingiber officinale Roscoe, in equal ratios. Some individual herbs present in Be...

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Autores principales:	Suchittra Samuhasaneeto, Gorawit Yusakul
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Bejakul Cytochrome P450 CYP2C11 CYP3A1 Herb-drug interaction Science (General) Q1-390 Social sciences (General) H1-99
Acceso en línea:	https://doaj.org/article/8f4a7d731b494a89a4e541ce6cb4d222
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spelling	oai:doaj.org-article:8f4a7d731b494a89a4e541ce6cb4d2222021-12-02T05:03:27ZModulatory effects of Benjakul extract on rat hepatic cytochrome P450 enzymes2405-844010.1016/j.heliyon.2021.e08498https://doaj.org/article/8f4a7d731b494a89a4e541ce6cb4d2222021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2405844021026013https://doaj.org/toc/2405-8440Benjakul, a traditional Thai formulation, has been used as a carminative and adaptogenic drug. It consists of five plants, Piper chaba Hunter, Piper sarmentosum Roxb., Piper interruptum Opiz., Plumbago indica Linn., and Zingiber officinale Roscoe, in equal ratios. Some individual herbs present in Benjakul were reported to modulate cytochrome P450 (CYP) enzymes. This study aimed to investigate the effects of Benjakul extract on the activities and mRNA expression levels of hepatic CYP2C11 and CYP3A1 in rats. Adult male rats were orally administered 200, 400, or 600 mg/kg BW Benjakul extract for 28 days. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN) and creatinine levels were assayed. CYP2C11 and CYP3A1 activities were analyzed using cytochrome P450 assay kits. The mRNA expression of CYP2C11 and CYP3A1 was measured using a quantitative real-time PCR assay. Benjakul treatment significantly increased the serum ALT and BUN levels. At doses of 200, 400, and 600 mg/kg BW, Benjakul treatment increased hepatic CYP3A1 activity and CYP3A1 mRNA expression. CYP2C11 mRNA expression was unchanged by treatment with Benjakul extract; however, treatment with the high and middle doses of Benjakul extract increased CYP2C11 activity. Treament with Benjakul extract induced CYP2C11 and CYP3A1 activity in rats. Concurrent use of Benjakul with conventional drugs should be considered to potentially induce herb-drug interactions.Suchittra SamuhasaneetoGorawit YusakulElsevierarticleBejakulCytochrome P450CYP2C11CYP3A1Herb-drug interactionScience (General)Q1-390Social sciences (General)H1-99ENHeliyon, Vol 7, Iss 11, Pp e08498- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Bejakul Cytochrome P450 CYP2C11 CYP3A1 Herb-drug interaction Science (General) Q1-390 Social sciences (General) H1-99
spellingShingle	Bejakul Cytochrome P450 CYP2C11 CYP3A1 Herb-drug interaction Science (General) Q1-390 Social sciences (General) H1-99 Suchittra Samuhasaneeto Gorawit Yusakul Modulatory effects of Benjakul extract on rat hepatic cytochrome P450 enzymes
description	Benjakul, a traditional Thai formulation, has been used as a carminative and adaptogenic drug. It consists of five plants, Piper chaba Hunter, Piper sarmentosum Roxb., Piper interruptum Opiz., Plumbago indica Linn., and Zingiber officinale Roscoe, in equal ratios. Some individual herbs present in Benjakul were reported to modulate cytochrome P450 (CYP) enzymes. This study aimed to investigate the effects of Benjakul extract on the activities and mRNA expression levels of hepatic CYP2C11 and CYP3A1 in rats. Adult male rats were orally administered 200, 400, or 600 mg/kg BW Benjakul extract for 28 days. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN) and creatinine levels were assayed. CYP2C11 and CYP3A1 activities were analyzed using cytochrome P450 assay kits. The mRNA expression of CYP2C11 and CYP3A1 was measured using a quantitative real-time PCR assay. Benjakul treatment significantly increased the serum ALT and BUN levels. At doses of 200, 400, and 600 mg/kg BW, Benjakul treatment increased hepatic CYP3A1 activity and CYP3A1 mRNA expression. CYP2C11 mRNA expression was unchanged by treatment with Benjakul extract; however, treatment with the high and middle doses of Benjakul extract increased CYP2C11 activity. Treament with Benjakul extract induced CYP2C11 and CYP3A1 activity in rats. Concurrent use of Benjakul with conventional drugs should be considered to potentially induce herb-drug interactions.
format	article
author	Suchittra Samuhasaneeto Gorawit Yusakul
author_facet	Suchittra Samuhasaneeto Gorawit Yusakul
author_sort	Suchittra Samuhasaneeto
title	Modulatory effects of Benjakul extract on rat hepatic cytochrome P450 enzymes
title_short	Modulatory effects of Benjakul extract on rat hepatic cytochrome P450 enzymes
title_full	Modulatory effects of Benjakul extract on rat hepatic cytochrome P450 enzymes
title_fullStr	Modulatory effects of Benjakul extract on rat hepatic cytochrome P450 enzymes
title_full_unstemmed	Modulatory effects of Benjakul extract on rat hepatic cytochrome P450 enzymes
title_sort	modulatory effects of benjakul extract on rat hepatic cytochrome p450 enzymes
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/8f4a7d731b494a89a4e541ce6cb4d222
work_keys_str_mv	AT suchittrasamuhasaneeto modulatoryeffectsofbenjakulextractonrathepaticcytochromep450enzymes AT gorawityusakul modulatoryeffectsofbenjakulextractonrathepaticcytochromep450enzymes
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Modulatory effects of Benjakul extract on rat hepatic cytochrome P450 enzymes

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