Bioinspired Nanofiber Scaffold for Differentiating Bone Marrow-Derived Neural Stem Cells to Oligodendrocyte-Like Cells: Design, Fabrication, and Characterization

Fatemeh Rasti Boroojeni,1,2 Shohreh Mashayekhan,1 Hojjat-Allah Abbaszadeh,3,4 Mohamadhasan Ansarizadeh,1,5 Maryam-Sadat Khoramgah,4 Vafa Rahimi Movaghar6 1Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, Iran; 2Division of Molecular Physics, Department of Ph...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Rasti Boroojeni F, Mashayekhan S, Abbaszadeh HA, Ansarizadeh M, Khoramgah MS, Rahimi Movaghar V
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	nanofibers scaffold oligodendrocyte cells controlled triiodothyronine release central nervous system polyaniline graphene Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/8f4eaa3e1a68435989adc9eb1bc9c3eb
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8f4eaa3e1a68435989adc9eb1bc9c3eb
record_format	dspace
institution	DOAJ
collection	DOAJ
language	EN
topic	nanofibers scaffold oligodendrocyte cells controlled triiodothyronine release central nervous system polyaniline graphene Medicine (General) R5-920
spellingShingle	nanofibers scaffold oligodendrocyte cells controlled triiodothyronine release central nervous system polyaniline graphene Medicine (General) R5-920 Rasti Boroojeni F Mashayekhan S Abbaszadeh HA Ansarizadeh M Khoramgah MS Rahimi Movaghar V Bioinspired Nanofiber Scaffold for Differentiating Bone Marrow-Derived Neural Stem Cells to Oligodendrocyte-Like Cells: Design, Fabrication, and Characterization
description	Fatemeh Rasti Boroojeni,1,2 Shohreh Mashayekhan,1 Hojjat-Allah Abbaszadeh,3,4 Mohamadhasan Ansarizadeh,1,5 Maryam-Sadat Khoramgah,4 Vafa Rahimi Movaghar6 1Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, Iran; 2Division of Molecular Physics, Department of Physics, Chemistry and Biology, Linköping University, Linköping, Sweden; 3Hearing Disorders Research Center, Loghman Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 4Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 5Biocenter Oulu, Faculty of Biochemistry and Molecular Medicine, University of Oulu, Oulu, Finland; 6Sina Trauma and Surgery Research Center, Tehran University of Medical Sciences, Tehran, IranCorrespondence: Hojjat-Allah AbbaszadehHearing Disorders Research Center, Loghman Hakim Hospital, Shahid Beheshti University of Medical Sciences, PO Box: 19395-4719, Tehran, Iran+98 21-22439976Email Dr.Abbaszadeh@sbmu.ac.irVafa Rahimi MovagharSina Trauma and Surgery Research Center, Tehran University of Medical Sciences, Tehran, IranTel +98 21-66757001Fax +98 21-66757009Email V_rahimi@sina.tums.ac.irBackground: Researchers are trying to study the mechanism of neural stem cells (NSCs) differentiation to oligodendrocyte-like cells (OLCs) as well as to enhance the selective differentiation of NSCs to oligodendrocytes. However, the limitation in nerve tissue accessibility to isolate the NSCs as well as their differentiation toward oligodendrocytes is still challenging.Purpose: In the present study, a hybrid polycaprolactone (PCL)-gelatin nanofiber scaffold mimicking the native extracellular matrix and axon morphology to direct the differentiation of bone marrow-derived NSCs to OLCs was introduced.Materials and Methods: In order to achieve a sustained release of T3, this factor was encapsulated within chitosan nanoparticles and chitosan-loaded T3 was incorporated within PCL nanofibers. Polyaniline graphene (PAG) nanocomposite was incorporated within gelatin nanofibers to endow the scaffold with conductive properties, which resemble the conductive behavior of axons. Biodegradation, water contact angle measurements, and scanning electron microscopy (SEM) observations as well as conductivity tests were used to evaluate the properties of the prepared scaffold. The concentration of PAG and T3-loaded chitosan NPs in nanofibers were optimized by examining the proliferation of cultured bone marrow-derived mesenchymal stem cells (BMSCs) on the scaffolds. The differentiation of BMSCs-derived NSCs cultured on the fabricated scaffolds into OLCs was analyzed by evaluating the expression of oligodendrocyte markers using immunofluorescence (ICC), RT-PCR and flowcytometric assays.Results: Incorporating 2% PAG proved to have superior cell support and proliferation while guaranteeing electrical conductivity of 10.8 × 10− 5 S/cm. Moreover, the scaffold containing 2% of T3-loaded chitosan NPs was considered to be the most biocompatible samples. Result of ICC, RT-PCR and flow cytometry showed high expression of O4, Olig2, platelet-derived growth factor receptor-alpha (PDGFR-α), O1, myelin/oligodendrocyte glycoprotein (MOG) and myelin basic protein (MBP) high expressed but low expression of glial fibrillary acidic protein (GFAP). Conclusion: Considering surface topography, biocompatibility, electrical conductivity and gene expression, the hybrid PCL/gelatin scaffold with the controlled release of T3 may be considered as a promising candidate to be used as an in vitro model to study patient-derived oligodendrocytes by isolating patient’s BMSCs in pathological conditions such as diseases or injuries. Moreover, the resulted oligodendrocytes can be used as a desirable source for transplanting in patients.Keywords: nanofibers scaffold, oligodendrocyte cells, controlled triiodothyronine release, central nervous system, polyaniline graphene
format	article
author	Rasti Boroojeni F Mashayekhan S Abbaszadeh HA Ansarizadeh M Khoramgah MS Rahimi Movaghar V
author_facet	Rasti Boroojeni F Mashayekhan S Abbaszadeh HA Ansarizadeh M Khoramgah MS Rahimi Movaghar V
author_sort	Rasti Boroojeni F
title	Bioinspired Nanofiber Scaffold for Differentiating Bone Marrow-Derived Neural Stem Cells to Oligodendrocyte-Like Cells: Design, Fabrication, and Characterization
title_short	Bioinspired Nanofiber Scaffold for Differentiating Bone Marrow-Derived Neural Stem Cells to Oligodendrocyte-Like Cells: Design, Fabrication, and Characterization
title_full	Bioinspired Nanofiber Scaffold for Differentiating Bone Marrow-Derived Neural Stem Cells to Oligodendrocyte-Like Cells: Design, Fabrication, and Characterization
title_fullStr	Bioinspired Nanofiber Scaffold for Differentiating Bone Marrow-Derived Neural Stem Cells to Oligodendrocyte-Like Cells: Design, Fabrication, and Characterization
title_full_unstemmed	Bioinspired Nanofiber Scaffold for Differentiating Bone Marrow-Derived Neural Stem Cells to Oligodendrocyte-Like Cells: Design, Fabrication, and Characterization
title_sort	bioinspired nanofiber scaffold for differentiating bone marrow-derived neural stem cells to oligodendrocyte-like cells: design, fabrication, and characterization
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/8f4eaa3e1a68435989adc9eb1bc9c3eb
work_keys_str_mv	AT rastiboroojenif bioinspirednanofiberscaffoldfordifferentiatingbonemarrowderivedneuralstemcellstooligodendrocytelikecellsdesignfabricationandcharacterization AT mashayekhans bioinspirednanofiberscaffoldfordifferentiatingbonemarrowderivedneuralstemcellstooligodendrocytelikecellsdesignfabricationandcharacterization AT abbaszadehha bioinspirednanofiberscaffoldfordifferentiatingbonemarrowderivedneuralstemcellstooligodendrocytelikecellsdesignfabricationandcharacterization AT ansarizadehm bioinspirednanofiberscaffoldfordifferentiatingbonemarrowderivedneuralstemcellstooligodendrocytelikecellsdesignfabricationandcharacterization AT khoramgahms bioinspirednanofiberscaffoldfordifferentiatingbonemarrowderivedneuralstemcellstooligodendrocytelikecellsdesignfabricationandcharacterization AT rahimimovagharv bioinspirednanofiberscaffoldfordifferentiatingbonemarrowderivedneuralstemcellstooligodendrocytelikecellsdesignfabricationandcharacterization
_version_	1718397809636409344
spelling	oai:doaj.org-article:8f4eaa3e1a68435989adc9eb1bc9c3eb2021-12-02T10:00:47ZBioinspired Nanofiber Scaffold for Differentiating Bone Marrow-Derived Neural Stem Cells to Oligodendrocyte-Like Cells: Design, Fabrication, and Characterization1178-2013https://doaj.org/article/8f4eaa3e1a68435989adc9eb1bc9c3eb2020-06-01T00:00:00Zhttps://www.dovepress.com/bioinspired-nanofiber-scaffold-for-differentiating-bone-marrow-derived-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Fatemeh Rasti Boroojeni,1,2 Shohreh Mashayekhan,1 Hojjat-Allah Abbaszadeh,3,4 Mohamadhasan Ansarizadeh,1,5 Maryam-Sadat Khoramgah,4 Vafa Rahimi Movaghar6 1Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, Iran; 2Division of Molecular Physics, Department of Physics, Chemistry and Biology, Linköping University, Linköping, Sweden; 3Hearing Disorders Research Center, Loghman Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 4Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran; 5Biocenter Oulu, Faculty of Biochemistry and Molecular Medicine, University of Oulu, Oulu, Finland; 6Sina Trauma and Surgery Research Center, Tehran University of Medical Sciences, Tehran, IranCorrespondence: Hojjat-Allah AbbaszadehHearing Disorders Research Center, Loghman Hakim Hospital, Shahid Beheshti University of Medical Sciences, PO Box: 19395-4719, Tehran, Iran+98 21-22439976Email Dr.Abbaszadeh@sbmu.ac.irVafa Rahimi MovagharSina Trauma and Surgery Research Center, Tehran University of Medical Sciences, Tehran, IranTel +98 21-66757001Fax +98 21-66757009Email V_rahimi@sina.tums.ac.irBackground: Researchers are trying to study the mechanism of neural stem cells (NSCs) differentiation to oligodendrocyte-like cells (OLCs) as well as to enhance the selective differentiation of NSCs to oligodendrocytes. However, the limitation in nerve tissue accessibility to isolate the NSCs as well as their differentiation toward oligodendrocytes is still challenging.Purpose: In the present study, a hybrid polycaprolactone (PCL)-gelatin nanofiber scaffold mimicking the native extracellular matrix and axon morphology to direct the differentiation of bone marrow-derived NSCs to OLCs was introduced.Materials and Methods: In order to achieve a sustained release of T3, this factor was encapsulated within chitosan nanoparticles and chitosan-loaded T3 was incorporated within PCL nanofibers. Polyaniline graphene (PAG) nanocomposite was incorporated within gelatin nanofibers to endow the scaffold with conductive properties, which resemble the conductive behavior of axons. Biodegradation, water contact angle measurements, and scanning electron microscopy (SEM) observations as well as conductivity tests were used to evaluate the properties of the prepared scaffold. The concentration of PAG and T3-loaded chitosan NPs in nanofibers were optimized by examining the proliferation of cultured bone marrow-derived mesenchymal stem cells (BMSCs) on the scaffolds. The differentiation of BMSCs-derived NSCs cultured on the fabricated scaffolds into OLCs was analyzed by evaluating the expression of oligodendrocyte markers using immunofluorescence (ICC), RT-PCR and flowcytometric assays.Results: Incorporating 2% PAG proved to have superior cell support and proliferation while guaranteeing electrical conductivity of 10.8 × 10− 5 S/cm. Moreover, the scaffold containing 2% of T3-loaded chitosan NPs was considered to be the most biocompatible samples. Result of ICC, RT-PCR and flow cytometry showed high expression of O4, Olig2, platelet-derived growth factor receptor-alpha (PDGFR-α), O1, myelin/oligodendrocyte glycoprotein (MOG) and myelin basic protein (MBP) high expressed but low expression of glial fibrillary acidic protein (GFAP). Conclusion: Considering surface topography, biocompatibility, electrical conductivity and gene expression, the hybrid PCL/gelatin scaffold with the controlled release of T3 may be considered as a promising candidate to be used as an in vitro model to study patient-derived oligodendrocytes by isolating patient’s BMSCs in pathological conditions such as diseases or injuries. Moreover, the resulted oligodendrocytes can be used as a desirable source for transplanting in patients.Keywords: nanofibers scaffold, oligodendrocyte cells, controlled triiodothyronine release, central nervous system, polyaniline grapheneRasti Boroojeni FMashayekhan SAbbaszadeh HAAnsarizadeh MKhoramgah MSRahimi Movaghar VDove Medical Pressarticlenanofibers scaffoldoligodendrocyte cellscontrolled triiodothyronine releasecentral nervous systempolyaniline grapheneMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 3903-3920 (2020)

Bioinspired Nanofiber Scaffold for Differentiating Bone Marrow-Derived Neural Stem Cells to Oligodendrocyte-Like Cells: Design, Fabrication, and Characterization

Ejemplares similares