Field evaluation of PGP Bacillus sp. strain D5 native to Crocus sativus, in traditional and non traditional areas, and mining of PGP genes from its genome
Abstract Native Bacillus sp. strain D5 coded as (Bar D5) has been isolated from the saffron corm that showed plant growth promotion (PGP) properties and also inhibits the growth of corm rot causing Fusarium oxysporum R1 (Fox R1) in-vitro. Bar D5 was more efficient PGP bacterium in comparison to earl...
Guardado en:
| Autores principales: | , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/8f5723bc35be421e857b16b5ef33d752 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:8f5723bc35be421e857b16b5ef33d752 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:8f5723bc35be421e857b16b5ef33d7522021-12-02T11:35:41ZField evaluation of PGP Bacillus sp. strain D5 native to Crocus sativus, in traditional and non traditional areas, and mining of PGP genes from its genome10.1038/s41598-021-84585-z2045-2322https://doaj.org/article/8f5723bc35be421e857b16b5ef33d7522021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84585-zhttps://doaj.org/toc/2045-2322Abstract Native Bacillus sp. strain D5 coded as (Bar D5) has been isolated from the saffron corm that showed plant growth promotion (PGP) properties and also inhibits the growth of corm rot causing Fusarium oxysporum R1 (Fox R1) in-vitro. Bar D5 was more efficient PGP bacterium in comparison to earlier reported native bio-formulations by our group. Pot assays and field evaluation of Bar D5 confirmed its in-vivo efficacy for PGP traits and biocontrol activity as well. Pot trials were followed by field trials at traditional (Kishtwar) and non-traditional (R.S Pura) saffron cultivation areas in Jammu and Kashmir. At both places, Bar D5 bio-formulation treatment led to the increase in root number & length, shoot number & length, flower number and number & weight of daughter corms. Additionally, it also decreased the corm rot disease incidence significantly. Priming of corms with bio-formulation resulted in the reduction of pathogenic fungal load by three fold at the depth of corm sowing from ground level. The shelf life/viability of Bar D5 based bio-formulation was found to be 52% (viable spores) for one year at room temperature. Draft genome sequence of Bar D5 revealed the presence of genes necessary for PGP and biocontrol activity. Further, confirmation of gene sequences and annotation was done by amplification, re-sequencing and mapping of PGP and biocontrol genes on draft genome. Bar D5 based bio-formulation can be provided to companies/researchers interested in saffron cultivation or bio-formulation production for commercial exploitation, since saffron is grown as revenue crop across continents. The present study bridges the gap between genomics and its field application.Shanu MagotraNancy BhagatSheetal AmbardarTahir AliBarbara Reinhold HurekThomas HurekPraveen Kumar VermaJyoti VakhluNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Shanu Magotra Nancy Bhagat Sheetal Ambardar Tahir Ali Barbara Reinhold Hurek Thomas Hurek Praveen Kumar Verma Jyoti Vakhlu Field evaluation of PGP Bacillus sp. strain D5 native to Crocus sativus, in traditional and non traditional areas, and mining of PGP genes from its genome |
| description |
Abstract Native Bacillus sp. strain D5 coded as (Bar D5) has been isolated from the saffron corm that showed plant growth promotion (PGP) properties and also inhibits the growth of corm rot causing Fusarium oxysporum R1 (Fox R1) in-vitro. Bar D5 was more efficient PGP bacterium in comparison to earlier reported native bio-formulations by our group. Pot assays and field evaluation of Bar D5 confirmed its in-vivo efficacy for PGP traits and biocontrol activity as well. Pot trials were followed by field trials at traditional (Kishtwar) and non-traditional (R.S Pura) saffron cultivation areas in Jammu and Kashmir. At both places, Bar D5 bio-formulation treatment led to the increase in root number & length, shoot number & length, flower number and number & weight of daughter corms. Additionally, it also decreased the corm rot disease incidence significantly. Priming of corms with bio-formulation resulted in the reduction of pathogenic fungal load by three fold at the depth of corm sowing from ground level. The shelf life/viability of Bar D5 based bio-formulation was found to be 52% (viable spores) for one year at room temperature. Draft genome sequence of Bar D5 revealed the presence of genes necessary for PGP and biocontrol activity. Further, confirmation of gene sequences and annotation was done by amplification, re-sequencing and mapping of PGP and biocontrol genes on draft genome. Bar D5 based bio-formulation can be provided to companies/researchers interested in saffron cultivation or bio-formulation production for commercial exploitation, since saffron is grown as revenue crop across continents. The present study bridges the gap between genomics and its field application. |
| format |
article |
| author |
Shanu Magotra Nancy Bhagat Sheetal Ambardar Tahir Ali Barbara Reinhold Hurek Thomas Hurek Praveen Kumar Verma Jyoti Vakhlu |
| author_facet |
Shanu Magotra Nancy Bhagat Sheetal Ambardar Tahir Ali Barbara Reinhold Hurek Thomas Hurek Praveen Kumar Verma Jyoti Vakhlu |
| author_sort |
Shanu Magotra |
| title |
Field evaluation of PGP Bacillus sp. strain D5 native to Crocus sativus, in traditional and non traditional areas, and mining of PGP genes from its genome |
| title_short |
Field evaluation of PGP Bacillus sp. strain D5 native to Crocus sativus, in traditional and non traditional areas, and mining of PGP genes from its genome |
| title_full |
Field evaluation of PGP Bacillus sp. strain D5 native to Crocus sativus, in traditional and non traditional areas, and mining of PGP genes from its genome |
| title_fullStr |
Field evaluation of PGP Bacillus sp. strain D5 native to Crocus sativus, in traditional and non traditional areas, and mining of PGP genes from its genome |
| title_full_unstemmed |
Field evaluation of PGP Bacillus sp. strain D5 native to Crocus sativus, in traditional and non traditional areas, and mining of PGP genes from its genome |
| title_sort |
field evaluation of pgp bacillus sp. strain d5 native to crocus sativus, in traditional and non traditional areas, and mining of pgp genes from its genome |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/8f5723bc35be421e857b16b5ef33d752 |
| work_keys_str_mv |
AT shanumagotra fieldevaluationofpgpbacillusspstraind5nativetocrocussativusintraditionalandnontraditionalareasandminingofpgpgenesfromitsgenome AT nancybhagat fieldevaluationofpgpbacillusspstraind5nativetocrocussativusintraditionalandnontraditionalareasandminingofpgpgenesfromitsgenome AT sheetalambardar fieldevaluationofpgpbacillusspstraind5nativetocrocussativusintraditionalandnontraditionalareasandminingofpgpgenesfromitsgenome AT tahirali fieldevaluationofpgpbacillusspstraind5nativetocrocussativusintraditionalandnontraditionalareasandminingofpgpgenesfromitsgenome AT barbarareinholdhurek fieldevaluationofpgpbacillusspstraind5nativetocrocussativusintraditionalandnontraditionalareasandminingofpgpgenesfromitsgenome AT thomashurek fieldevaluationofpgpbacillusspstraind5nativetocrocussativusintraditionalandnontraditionalareasandminingofpgpgenesfromitsgenome AT praveenkumarverma fieldevaluationofpgpbacillusspstraind5nativetocrocussativusintraditionalandnontraditionalareasandminingofpgpgenesfromitsgenome AT jyotivakhlu fieldevaluationofpgpbacillusspstraind5nativetocrocussativusintraditionalandnontraditionalareasandminingofpgpgenesfromitsgenome |
| _version_ |
1718395832334548992 |