Generation of restriction endonucleases barcode map to trace SARS-CoV-2 origin and evolution

Abstract Since the first report of SARS-CoV-2 in China in 2019, there has been a huge debate about the origin. In this work, using a different method we aimed to strengthen the observation that no evidence of genetic manipulation has been found by (1) detecting classical restriction site (RS) sequen...

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Autores principales:	Federico Colombo, Elisa Corsiero, Myles J. Lewis, Costantino Pitzalis
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8f5ad5791f7140adafe410f90355b306
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spelling	oai:doaj.org-article:8f5ad5791f7140adafe410f90355b3062021-12-02T17:51:06ZGeneration of restriction endonucleases barcode map to trace SARS-CoV-2 origin and evolution10.1038/s41598-021-91264-62045-2322https://doaj.org/article/8f5ad5791f7140adafe410f90355b3062021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91264-6https://doaj.org/toc/2045-2322Abstract Since the first report of SARS-CoV-2 in China in 2019, there has been a huge debate about the origin. In this work, using a different method we aimed to strengthen the observation that no evidence of genetic manipulation has been found by (1) detecting classical restriction site (RS) sequence in human SARS-CoV-2 genomes and (2) comparing them with other recombinant SARS-CoV-like virus created for experimental purposes. Finally, we propose a novel approach consisting in the generation of a restriction endonucleases site map of SARS-CoV-2 and other related coronavirus genomes to be used as a fingerprint to trace the virus evolution.Federico ColomboElisa CorsieroMyles J. LewisCostantino PitzalisNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Federico Colombo Elisa Corsiero Myles J. Lewis Costantino Pitzalis Generation of restriction endonucleases barcode map to trace SARS-CoV-2 origin and evolution
description	Abstract Since the first report of SARS-CoV-2 in China in 2019, there has been a huge debate about the origin. In this work, using a different method we aimed to strengthen the observation that no evidence of genetic manipulation has been found by (1) detecting classical restriction site (RS) sequence in human SARS-CoV-2 genomes and (2) comparing them with other recombinant SARS-CoV-like virus created for experimental purposes. Finally, we propose a novel approach consisting in the generation of a restriction endonucleases site map of SARS-CoV-2 and other related coronavirus genomes to be used as a fingerprint to trace the virus evolution.
format	article
author	Federico Colombo Elisa Corsiero Myles J. Lewis Costantino Pitzalis
author_facet	Federico Colombo Elisa Corsiero Myles J. Lewis Costantino Pitzalis
author_sort	Federico Colombo
title	Generation of restriction endonucleases barcode map to trace SARS-CoV-2 origin and evolution
title_short	Generation of restriction endonucleases barcode map to trace SARS-CoV-2 origin and evolution
title_full	Generation of restriction endonucleases barcode map to trace SARS-CoV-2 origin and evolution
title_fullStr	Generation of restriction endonucleases barcode map to trace SARS-CoV-2 origin and evolution
title_full_unstemmed	Generation of restriction endonucleases barcode map to trace SARS-CoV-2 origin and evolution
title_sort	generation of restriction endonucleases barcode map to trace sars-cov-2 origin and evolution
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8f5ad5791f7140adafe410f90355b306
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Generation of restriction endonucleases barcode map to trace SARS-CoV-2 origin and evolution

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