Activation of the WNT-BMP-FGF Regulatory Network Induces the Onset of Cell Death in Anterior Mesodermal Cells to Establish the ANZ

The spatiotemporal control of programmed cell death (PCD) plays a significant role in sculpting the limb. In the early avian limb bud, the anterior necrotic zone (ANZ) and the posterior necrotic zone are two cell death regions associated with digit number reduction. In this study, we evaluated the f...

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Autores principales:	Martha Elena Díaz-Hernández, Claudio Iván Galván-Hernández, Jessica Cristina Marín-Llera, Karen Camargo-Sosa, Marcia Bustamante, Sabina Wischin, Jesús Chimal-Monroy
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	limb development ANZ Fgf signaling Wnt signaing BMP signaling programmed cell death Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/8f5aec2873a546e6bd02082189dd16ba
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spelling	oai:doaj.org-article:8f5aec2873a546e6bd02082189dd16ba2021-11-08T07:42:04ZActivation of the WNT-BMP-FGF Regulatory Network Induces the Onset of Cell Death in Anterior Mesodermal Cells to Establish the ANZ2296-634X10.3389/fcell.2021.703836https://doaj.org/article/8f5aec2873a546e6bd02082189dd16ba2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcell.2021.703836/fullhttps://doaj.org/toc/2296-634XThe spatiotemporal control of programmed cell death (PCD) plays a significant role in sculpting the limb. In the early avian limb bud, the anterior necrotic zone (ANZ) and the posterior necrotic zone are two cell death regions associated with digit number reduction. In this study, we evaluated the first events triggered by the FGF, BMP, and WNT signaling interactions to initiate cell death in the anterior margin of the limb to establish the ANZ. This study demonstrates that in a period of two to 8 h after the inhibition of WNT or FGF signaling or the activation of BMP signaling, cell death was induced in the anterior margin of the limb concomitantly with the regulation of Dkk, Fgf8, and Bmp4 expression. Comparing the gene expression profile between the ANZ and the undifferentiated zone at 22HH and 25HH and between the ANZ of 22HH and 25HH stages correlates with functional programs controlled by the regulatory network FGF, BMP, and WNT signaling in the anterior margin of the limb. This work provides novel insights to recognize a negative feedback loop between FGF8, BMP4, and DKK to control the onset of cell death in the anterior margin of the limb to the establishment of the ANZ.Martha Elena Díaz-HernándezClaudio Iván Galván-HernándezJessica Cristina Marín-LleraKaren Camargo-SosaMarcia BustamanteSabina WischinJesús Chimal-MonroyFrontiers Media S.A.articlelimb developmentANZFgf signalingWnt signaingBMP signalingprogrammed cell deathBiology (General)QH301-705.5ENFrontiers in Cell and Developmental Biology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	limb development ANZ Fgf signaling Wnt signaing BMP signaling programmed cell death Biology (General) QH301-705.5
spellingShingle	limb development ANZ Fgf signaling Wnt signaing BMP signaling programmed cell death Biology (General) QH301-705.5 Martha Elena Díaz-Hernández Claudio Iván Galván-Hernández Jessica Cristina Marín-Llera Karen Camargo-Sosa Marcia Bustamante Sabina Wischin Jesús Chimal-Monroy Activation of the WNT-BMP-FGF Regulatory Network Induces the Onset of Cell Death in Anterior Mesodermal Cells to Establish the ANZ
description	The spatiotemporal control of programmed cell death (PCD) plays a significant role in sculpting the limb. In the early avian limb bud, the anterior necrotic zone (ANZ) and the posterior necrotic zone are two cell death regions associated with digit number reduction. In this study, we evaluated the first events triggered by the FGF, BMP, and WNT signaling interactions to initiate cell death in the anterior margin of the limb to establish the ANZ. This study demonstrates that in a period of two to 8 h after the inhibition of WNT or FGF signaling or the activation of BMP signaling, cell death was induced in the anterior margin of the limb concomitantly with the regulation of Dkk, Fgf8, and Bmp4 expression. Comparing the gene expression profile between the ANZ and the undifferentiated zone at 22HH and 25HH and between the ANZ of 22HH and 25HH stages correlates with functional programs controlled by the regulatory network FGF, BMP, and WNT signaling in the anterior margin of the limb. This work provides novel insights to recognize a negative feedback loop between FGF8, BMP4, and DKK to control the onset of cell death in the anterior margin of the limb to the establishment of the ANZ.
format	article
author	Martha Elena Díaz-Hernández Claudio Iván Galván-Hernández Jessica Cristina Marín-Llera Karen Camargo-Sosa Marcia Bustamante Sabina Wischin Jesús Chimal-Monroy
author_facet	Martha Elena Díaz-Hernández Claudio Iván Galván-Hernández Jessica Cristina Marín-Llera Karen Camargo-Sosa Marcia Bustamante Sabina Wischin Jesús Chimal-Monroy
author_sort	Martha Elena Díaz-Hernández
title	Activation of the WNT-BMP-FGF Regulatory Network Induces the Onset of Cell Death in Anterior Mesodermal Cells to Establish the ANZ
title_short	Activation of the WNT-BMP-FGF Regulatory Network Induces the Onset of Cell Death in Anterior Mesodermal Cells to Establish the ANZ
title_full	Activation of the WNT-BMP-FGF Regulatory Network Induces the Onset of Cell Death in Anterior Mesodermal Cells to Establish the ANZ
title_fullStr	Activation of the WNT-BMP-FGF Regulatory Network Induces the Onset of Cell Death in Anterior Mesodermal Cells to Establish the ANZ
title_full_unstemmed	Activation of the WNT-BMP-FGF Regulatory Network Induces the Onset of Cell Death in Anterior Mesodermal Cells to Establish the ANZ
title_sort	activation of the wnt-bmp-fgf regulatory network induces the onset of cell death in anterior mesodermal cells to establish the anz
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/8f5aec2873a546e6bd02082189dd16ba
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Activation of the WNT-BMP-FGF Regulatory Network Induces the Onset of Cell Death in Anterior Mesodermal Cells to Establish the ANZ

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