3D bioprinting via an in situ crosslinking technique towards engineering cartilage tissue

Abstract 3D bioprinting is a promising approach for the repair of cartilage tissue after damage due to injury or disease; however, the design of 3D printed scaffolds has been limited by the availability of bioinks with requisite printability, cytocompatibility, and bioactivity. To address this, we d...

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Autores principales:	Jonathan H. Galarraga, Mi Y. Kwon, Jason A. Burdick
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8f642da163654c7bb22463da110983c6
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spelling	oai:doaj.org-article:8f642da163654c7bb22463da110983c62021-12-02T13:35:03Z3D bioprinting via an in situ crosslinking technique towards engineering cartilage tissue10.1038/s41598-019-56117-32045-2322https://doaj.org/article/8f642da163654c7bb22463da110983c62019-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-56117-3https://doaj.org/toc/2045-2322Abstract 3D bioprinting is a promising approach for the repair of cartilage tissue after damage due to injury or disease; however, the design of 3D printed scaffolds has been limited by the availability of bioinks with requisite printability, cytocompatibility, and bioactivity. To address this, we developed an approach termed in situ crosslinking that permits the printing of non-viscous, photocrosslinkable bioinks via the direct-curing of the bioink with light through a photopermeable capillary prior to deposition. Using a norbornene-modified hyaluronic acid (NorHA) macromer as a representative bioink and our understanding of thiol-ene curing kinetics with visible light, we varied the printing parameters (e.g., capillary length, flow rate, light intensity) to identify printing conditions that were optimal for the ink. The printing process was cytocompatible, with high cell viability and homogenous distribution of mesenchymal stromal cells (MSCs) observed throughout printed constructs. Over 56 days of culture in chondrogenic media, printed constructs increased in compressive moduli, biochemical content (i.e., sulfated glycosaminoglycans, collagen), and histological staining of matrix associated with cartilage tissue. This generalizable printing approach may be used towards the repair of focal defects in articular cartilage or broadly towards widespread biomedical applications across a range of photocrosslinkable bioinks that can now be printed.Jonathan H. GalarragaMi Y. KwonJason A. BurdickNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-12 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jonathan H. Galarraga Mi Y. Kwon Jason A. Burdick 3D bioprinting via an in situ crosslinking technique towards engineering cartilage tissue
description	Abstract 3D bioprinting is a promising approach for the repair of cartilage tissue after damage due to injury or disease; however, the design of 3D printed scaffolds has been limited by the availability of bioinks with requisite printability, cytocompatibility, and bioactivity. To address this, we developed an approach termed in situ crosslinking that permits the printing of non-viscous, photocrosslinkable bioinks via the direct-curing of the bioink with light through a photopermeable capillary prior to deposition. Using a norbornene-modified hyaluronic acid (NorHA) macromer as a representative bioink and our understanding of thiol-ene curing kinetics with visible light, we varied the printing parameters (e.g., capillary length, flow rate, light intensity) to identify printing conditions that were optimal for the ink. The printing process was cytocompatible, with high cell viability and homogenous distribution of mesenchymal stromal cells (MSCs) observed throughout printed constructs. Over 56 days of culture in chondrogenic media, printed constructs increased in compressive moduli, biochemical content (i.e., sulfated glycosaminoglycans, collagen), and histological staining of matrix associated with cartilage tissue. This generalizable printing approach may be used towards the repair of focal defects in articular cartilage or broadly towards widespread biomedical applications across a range of photocrosslinkable bioinks that can now be printed.
format	article
author	Jonathan H. Galarraga Mi Y. Kwon Jason A. Burdick
author_facet	Jonathan H. Galarraga Mi Y. Kwon Jason A. Burdick
author_sort	Jonathan H. Galarraga
title	3D bioprinting via an in situ crosslinking technique towards engineering cartilage tissue
title_short	3D bioprinting via an in situ crosslinking technique towards engineering cartilage tissue
title_full	3D bioprinting via an in situ crosslinking technique towards engineering cartilage tissue
title_fullStr	3D bioprinting via an in situ crosslinking technique towards engineering cartilage tissue
title_full_unstemmed	3D bioprinting via an in situ crosslinking technique towards engineering cartilage tissue
title_sort	3d bioprinting via an in situ crosslinking technique towards engineering cartilage tissue
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/8f642da163654c7bb22463da110983c6
work_keys_str_mv	AT jonathanhgalarraga 3dbioprintingviaaninsitucrosslinkingtechniquetowardsengineeringcartilagetissue AT miykwon 3dbioprintingviaaninsitucrosslinkingtechniquetowardsengineeringcartilagetissue AT jasonaburdick 3dbioprintingviaaninsitucrosslinkingtechniquetowardsengineeringcartilagetissue
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3D bioprinting via an in situ crosslinking technique towards engineering cartilage tissue

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