CtIP is required to initiate replication-dependent interstrand crosslink repair.

DNA interstrand crosslinks (ICLs) are toxic lesions that block the progression of replication and transcription. CtIP is a conserved DNA repair protein that facilitates DNA end resection in the double-strand break (DSB) repair pathway. Here we show that CtIP plays a critical role during initiation o...

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Autores principales:	Michelle L Duquette, Qingyuan Zhu, Ewan R Taylor, Angela J Tsay, Linda Z Shi, Michael W Berns, Clare H McGowan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/8f6f239b578c43518c801b93d319f176
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spelling	oai:doaj.org-article:8f6f239b578c43518c801b93d319f1762021-11-18T06:20:34ZCtIP is required to initiate replication-dependent interstrand crosslink repair.1553-73901553-740410.1371/journal.pgen.1003050https://doaj.org/article/8f6f239b578c43518c801b93d319f1762012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23144634/?tool=EBIhttps://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404DNA interstrand crosslinks (ICLs) are toxic lesions that block the progression of replication and transcription. CtIP is a conserved DNA repair protein that facilitates DNA end resection in the double-strand break (DSB) repair pathway. Here we show that CtIP plays a critical role during initiation of ICL processing in replicating human cells that is distinct from its role in DSB repair. CtIP depletion sensitizes human cells to ICL inducing agents and significantly impairs the accumulation of DNA damage response proteins RPA, ATR, FANCD2, γH2AX, and phosphorylated ATM at sites of laser generated ICLs. In contrast, the appearance of γH2AX and phosphorylated ATM at sites of laser generated double strand breaks (DSBs) is CtIP-independent. We present a model in which CtIP functions early in ICL repair in a BRCA1- and FANCM-dependent manner prior to generation of DSB repair intermediates.Michelle L DuquetteQingyuan ZhuEwan R TaylorAngela J TsayLinda Z ShiMichael W BernsClare H McGowanPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 8, Iss 11, p e1003050 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Michelle L Duquette Qingyuan Zhu Ewan R Taylor Angela J Tsay Linda Z Shi Michael W Berns Clare H McGowan CtIP is required to initiate replication-dependent interstrand crosslink repair.
description	DNA interstrand crosslinks (ICLs) are toxic lesions that block the progression of replication and transcription. CtIP is a conserved DNA repair protein that facilitates DNA end resection in the double-strand break (DSB) repair pathway. Here we show that CtIP plays a critical role during initiation of ICL processing in replicating human cells that is distinct from its role in DSB repair. CtIP depletion sensitizes human cells to ICL inducing agents and significantly impairs the accumulation of DNA damage response proteins RPA, ATR, FANCD2, γH2AX, and phosphorylated ATM at sites of laser generated ICLs. In contrast, the appearance of γH2AX and phosphorylated ATM at sites of laser generated double strand breaks (DSBs) is CtIP-independent. We present a model in which CtIP functions early in ICL repair in a BRCA1- and FANCM-dependent manner prior to generation of DSB repair intermediates.
format	article
author	Michelle L Duquette Qingyuan Zhu Ewan R Taylor Angela J Tsay Linda Z Shi Michael W Berns Clare H McGowan
author_facet	Michelle L Duquette Qingyuan Zhu Ewan R Taylor Angela J Tsay Linda Z Shi Michael W Berns Clare H McGowan
author_sort	Michelle L Duquette
title	CtIP is required to initiate replication-dependent interstrand crosslink repair.
title_short	CtIP is required to initiate replication-dependent interstrand crosslink repair.
title_full	CtIP is required to initiate replication-dependent interstrand crosslink repair.
title_fullStr	CtIP is required to initiate replication-dependent interstrand crosslink repair.
title_full_unstemmed	CtIP is required to initiate replication-dependent interstrand crosslink repair.
title_sort	ctip is required to initiate replication-dependent interstrand crosslink repair.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/8f6f239b578c43518c801b93d319f176
work_keys_str_mv	AT michellelduquette ctipisrequiredtoinitiatereplicationdependentinterstrandcrosslinkrepair AT qingyuanzhu ctipisrequiredtoinitiatereplicationdependentinterstrandcrosslinkrepair AT ewanrtaylor ctipisrequiredtoinitiatereplicationdependentinterstrandcrosslinkrepair AT angelajtsay ctipisrequiredtoinitiatereplicationdependentinterstrandcrosslinkrepair AT lindazshi ctipisrequiredtoinitiatereplicationdependentinterstrandcrosslinkrepair AT michaelwberns ctipisrequiredtoinitiatereplicationdependentinterstrandcrosslinkrepair AT clarehmcgowan ctipisrequiredtoinitiatereplicationdependentinterstrandcrosslinkrepair
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CtIP is required to initiate replication-dependent interstrand crosslink repair.

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