Systematic Reconstruction of the Complete Two-Component Sensorial Network in <named-content content-type="genus-species">Staphylococcus aureus</named-content>

ABSTRACT In bacteria, adaptation to changes in the environment is mainly controlled through two-component signal transduction systems (TCSs). Most bacteria contain dozens of TCSs, each of them responsible for sensing a different range of signals and controlling the expression of a repertoire of targ...

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Autores principales:	B. Rapun-Araiz, A. F. Haag, V. De Cesare, C. Gil, P. Dorado-Morales, J. R. Penades, I. Lasa
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	Staphylococcus aureus regulon two-component systems Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/8f82edbfd1904e46a6a589a0f177ed94
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spelling	oai:doaj.org-article:8f82edbfd1904e46a6a589a0f177ed942021-12-02T18:44:37ZSystematic Reconstruction of the Complete Two-Component Sensorial Network in <named-content content-type="genus-species">Staphylococcus aureus</named-content>10.1128/mSystems.00511-202379-5077https://doaj.org/article/8f82edbfd1904e46a6a589a0f177ed942020-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00511-20https://doaj.org/toc/2379-5077ABSTRACT In bacteria, adaptation to changes in the environment is mainly controlled through two-component signal transduction systems (TCSs). Most bacteria contain dozens of TCSs, each of them responsible for sensing a different range of signals and controlling the expression of a repertoire of target genes (regulon). Over the years, identification of the regulon controlled by each individual TCS in different bacteria has been a recurrent question. However, limitations associated with the classical approaches used have left our knowledge far from complete. In this report, using a pioneering approach in which a strain devoid of the complete nonessential TCS network was systematically complemented with the constitutively active form of each response regulator, we have reconstituted the regulon of each TCS of S. aureus in the absence of interference between members of the family. Transcriptome sequencing (RNA-Seq) and proteomics allowed us to determine the size, complexity, and insulation of each regulon and to identify the genes regulated exclusively by one or many TCSs. This gain-of-function strategy provides the first description of the complete TCS regulon in a living cell, which we expect will be useful to understand the pathobiology of this important pathogen. IMPORTANCE Bacteria are able to sense environmental conditions and respond accordingly. Their sensorial system relies on pairs of sensory and regulatory proteins, known as two-component systems (TCSs). The majority of bacteria contain dozens of TCSs, each of them responsible for sensing and responding to a different range of signals. Traditionally, the function of each TCS has been determined by analyzing the changes in gene expression caused by the absence of individual TCSs. Here, we used a bacterial strain deprived of the complete TC sensorial system to introduce, one by one, the active form of every TCS. This gain-of-function strategy allowed us to identify the changes in gene expression conferred by each TCS without interference of other members of the family.B. Rapun-AraizA. F. HaagV. De CesareC. GilP. Dorado-MoralesJ. R. PenadesI. LasaAmerican Society for MicrobiologyarticleStaphylococcus aureusregulontwo-component systemsMicrobiologyQR1-502ENmSystems, Vol 5, Iss 4 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Staphylococcus aureus regulon two-component systems Microbiology QR1-502
spellingShingle	Staphylococcus aureus regulon two-component systems Microbiology QR1-502 B. Rapun-Araiz A. F. Haag V. De Cesare C. Gil P. Dorado-Morales J. R. Penades I. Lasa Systematic Reconstruction of the Complete Two-Component Sensorial Network in <named-content content-type="genus-species">Staphylococcus aureus</named-content>
description	ABSTRACT In bacteria, adaptation to changes in the environment is mainly controlled through two-component signal transduction systems (TCSs). Most bacteria contain dozens of TCSs, each of them responsible for sensing a different range of signals and controlling the expression of a repertoire of target genes (regulon). Over the years, identification of the regulon controlled by each individual TCS in different bacteria has been a recurrent question. However, limitations associated with the classical approaches used have left our knowledge far from complete. In this report, using a pioneering approach in which a strain devoid of the complete nonessential TCS network was systematically complemented with the constitutively active form of each response regulator, we have reconstituted the regulon of each TCS of S. aureus in the absence of interference between members of the family. Transcriptome sequencing (RNA-Seq) and proteomics allowed us to determine the size, complexity, and insulation of each regulon and to identify the genes regulated exclusively by one or many TCSs. This gain-of-function strategy provides the first description of the complete TCS regulon in a living cell, which we expect will be useful to understand the pathobiology of this important pathogen. IMPORTANCE Bacteria are able to sense environmental conditions and respond accordingly. Their sensorial system relies on pairs of sensory and regulatory proteins, known as two-component systems (TCSs). The majority of bacteria contain dozens of TCSs, each of them responsible for sensing and responding to a different range of signals. Traditionally, the function of each TCS has been determined by analyzing the changes in gene expression caused by the absence of individual TCSs. Here, we used a bacterial strain deprived of the complete TC sensorial system to introduce, one by one, the active form of every TCS. This gain-of-function strategy allowed us to identify the changes in gene expression conferred by each TCS without interference of other members of the family.
format	article
author	B. Rapun-Araiz A. F. Haag V. De Cesare C. Gil P. Dorado-Morales J. R. Penades I. Lasa
author_facet	B. Rapun-Araiz A. F. Haag V. De Cesare C. Gil P. Dorado-Morales J. R. Penades I. Lasa
author_sort	B. Rapun-Araiz
title	Systematic Reconstruction of the Complete Two-Component Sensorial Network in <named-content content-type="genus-species">Staphylococcus aureus</named-content>
title_short	Systematic Reconstruction of the Complete Two-Component Sensorial Network in <named-content content-type="genus-species">Staphylococcus aureus</named-content>
title_full	Systematic Reconstruction of the Complete Two-Component Sensorial Network in <named-content content-type="genus-species">Staphylococcus aureus</named-content>
title_fullStr	Systematic Reconstruction of the Complete Two-Component Sensorial Network in <named-content content-type="genus-species">Staphylococcus aureus</named-content>
title_full_unstemmed	Systematic Reconstruction of the Complete Two-Component Sensorial Network in <named-content content-type="genus-species">Staphylococcus aureus</named-content>
title_sort	systematic reconstruction of the complete two-component sensorial network in <named-content content-type="genus-species">staphylococcus aureus</named-content>
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/8f82edbfd1904e46a6a589a0f177ed94
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Systematic Reconstruction of the Complete Two-Component Sensorial Network in <named-content content-type="genus-species">Staphylococcus aureus</named-content>

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