Connecting variability in global transcription rate to mitochondrial variability.

Populations of genetically identical eukaryotic cells show significant cell-to-cell variability in gene expression. However, we lack a good understanding of the origins of this variation. We have found marked cell-to-cell variability in average cellular rates of transcription. We also found marked c...

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Autores principales:	Ricardo Pires das Neves, Nick S Jones, Lorena Andreu, Rajeev Gupta, Tariq Enver, Francisco J Iborra
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/8f8a6b3d7bb942b2a6057da38c626b02
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spelling	oai:doaj.org-article:8f8a6b3d7bb942b2a6057da38c626b022021-11-18T05:36:25ZConnecting variability in global transcription rate to mitochondrial variability.1544-91731545-788510.1371/journal.pbio.1000560https://doaj.org/article/8f8a6b3d7bb942b2a6057da38c626b022010-12-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21179497/pdf/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885Populations of genetically identical eukaryotic cells show significant cell-to-cell variability in gene expression. However, we lack a good understanding of the origins of this variation. We have found marked cell-to-cell variability in average cellular rates of transcription. We also found marked cell-to-cell variability in the amount of cellular mitochondrial mass. We undertook fusion studies that suggested that variability in transcription rate depends on small diffusible factors. Following this, in vitro studies showed that transcription rate has a sensitive dependence on [ATP] but not on the concentration of other nucleotide triphosphates (NTPs). Further experiments that perturbed populations by changing nutrient levels and available [ATP] suggested this connection holds in vivo. We found evidence that cells with higher mitochondrial mass, or higher total membrane potential, have a faster rate of transcription per unit volume of nuclear material. We also found evidence that transcription rate variability is substantially modulated by the presence of anti- or prooxidants. Daughter studies showed that a cause of variability in mitochondrial content is apparently stochastic segregation of mitochondria at division. We conclude by noting that daughters that stochastically inherit a lower mitochondrial mass than their sisters have relatively longer cell cycles. Our findings reveal a link between variability in energy metabolism and variability in transcription rate.Ricardo Pires das NevesNick S JonesLorena AndreuRajeev GuptaTariq EnverFrancisco J IborraPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 8, Iss 12, p e1000560 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Ricardo Pires das Neves Nick S Jones Lorena Andreu Rajeev Gupta Tariq Enver Francisco J Iborra Connecting variability in global transcription rate to mitochondrial variability.
description	Populations of genetically identical eukaryotic cells show significant cell-to-cell variability in gene expression. However, we lack a good understanding of the origins of this variation. We have found marked cell-to-cell variability in average cellular rates of transcription. We also found marked cell-to-cell variability in the amount of cellular mitochondrial mass. We undertook fusion studies that suggested that variability in transcription rate depends on small diffusible factors. Following this, in vitro studies showed that transcription rate has a sensitive dependence on [ATP] but not on the concentration of other nucleotide triphosphates (NTPs). Further experiments that perturbed populations by changing nutrient levels and available [ATP] suggested this connection holds in vivo. We found evidence that cells with higher mitochondrial mass, or higher total membrane potential, have a faster rate of transcription per unit volume of nuclear material. We also found evidence that transcription rate variability is substantially modulated by the presence of anti- or prooxidants. Daughter studies showed that a cause of variability in mitochondrial content is apparently stochastic segregation of mitochondria at division. We conclude by noting that daughters that stochastically inherit a lower mitochondrial mass than their sisters have relatively longer cell cycles. Our findings reveal a link between variability in energy metabolism and variability in transcription rate.
format	article
author	Ricardo Pires das Neves Nick S Jones Lorena Andreu Rajeev Gupta Tariq Enver Francisco J Iborra
author_facet	Ricardo Pires das Neves Nick S Jones Lorena Andreu Rajeev Gupta Tariq Enver Francisco J Iborra
author_sort	Ricardo Pires das Neves
title	Connecting variability in global transcription rate to mitochondrial variability.
title_short	Connecting variability in global transcription rate to mitochondrial variability.
title_full	Connecting variability in global transcription rate to mitochondrial variability.
title_fullStr	Connecting variability in global transcription rate to mitochondrial variability.
title_full_unstemmed	Connecting variability in global transcription rate to mitochondrial variability.
title_sort	connecting variability in global transcription rate to mitochondrial variability.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/8f8a6b3d7bb942b2a6057da38c626b02
work_keys_str_mv	AT ricardopiresdasneves connectingvariabilityinglobaltranscriptionratetomitochondrialvariability AT nicksjones connectingvariabilityinglobaltranscriptionratetomitochondrialvariability AT lorenaandreu connectingvariabilityinglobaltranscriptionratetomitochondrialvariability AT rajeevgupta connectingvariabilityinglobaltranscriptionratetomitochondrialvariability AT tariqenver connectingvariabilityinglobaltranscriptionratetomitochondrialvariability AT franciscojiborra connectingvariabilityinglobaltranscriptionratetomitochondrialvariability
_version_	1718424926465032192

Connecting variability in global transcription rate to mitochondrial variability.

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