Functional expression of an oxygen-labile nitrogenase in an oxygenic photosynthetic organism

Abstract Transfer of nitrogen fixation ability to plants, especially crops, is a promising approach to mitigate dependence on chemical nitrogen fertilizer and alleviate environmental pollution caused by nitrogen fertilizer run-off. However, the need to transfer a large number of nitrogen fixation (n...

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Autores principales:	Ryoma Tsujimoto, Hiroya Kotani, Konomi Yokomizo, Hisanori Yamakawa, Aoi Nonaka, Yuichi Fujita
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8f8bfde25596419d9e28912d38902419
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spelling	oai:doaj.org-article:8f8bfde25596419d9e28912d389024192021-12-02T16:08:03ZFunctional expression of an oxygen-labile nitrogenase in an oxygenic photosynthetic organism10.1038/s41598-018-25396-72045-2322https://doaj.org/article/8f8bfde25596419d9e28912d389024192018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25396-7https://doaj.org/toc/2045-2322Abstract Transfer of nitrogen fixation ability to plants, especially crops, is a promising approach to mitigate dependence on chemical nitrogen fertilizer and alleviate environmental pollution caused by nitrogen fertilizer run-off. However, the need to transfer a large number of nitrogen fixation (nif) genes and the extreme vulnerability of nitrogenase to oxygen constitute major obstacles for transfer of nitrogen-fixing ability to plants. Here we demonstrate functional expression of a cyanobacterial nitrogenase in the non-diazotrophic cyanobacterium Synechocystis sp. PCC 6803 (Synechocystis 6803). A 20.8-kb chromosomal fragment containing 25 nif and nif-related genes of the diazotrophic cyanobacterium Leptolyngbya boryana was integrated into a neutral genome site of Synechocystis 6803 by five-step homologous recombination together with the cnfR gene encoding the transcriptional activator of the nif genes to isolate CN1. In addition, two other transformants CN2 and CN3 carrying additional one and four genes, respectively, were isolated from CN1. Low but significant nitrogenase activity was detected in all transformants. This is the first example of nitrogenase activity detected in non-diazotrophic photosynthetic organisms. These strains provide valuable platforms to investigate unknown factors that enable nitrogen-fixing growth of non-diazotrophic photosynthetic organisms, including plants.Ryoma TsujimotoHiroya KotaniKonomi YokomizoHisanori YamakawaAoi NonakaYuichi FujitaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ryoma Tsujimoto Hiroya Kotani Konomi Yokomizo Hisanori Yamakawa Aoi Nonaka Yuichi Fujita Functional expression of an oxygen-labile nitrogenase in an oxygenic photosynthetic organism
description	Abstract Transfer of nitrogen fixation ability to plants, especially crops, is a promising approach to mitigate dependence on chemical nitrogen fertilizer and alleviate environmental pollution caused by nitrogen fertilizer run-off. However, the need to transfer a large number of nitrogen fixation (nif) genes and the extreme vulnerability of nitrogenase to oxygen constitute major obstacles for transfer of nitrogen-fixing ability to plants. Here we demonstrate functional expression of a cyanobacterial nitrogenase in the non-diazotrophic cyanobacterium Synechocystis sp. PCC 6803 (Synechocystis 6803). A 20.8-kb chromosomal fragment containing 25 nif and nif-related genes of the diazotrophic cyanobacterium Leptolyngbya boryana was integrated into a neutral genome site of Synechocystis 6803 by five-step homologous recombination together with the cnfR gene encoding the transcriptional activator of the nif genes to isolate CN1. In addition, two other transformants CN2 and CN3 carrying additional one and four genes, respectively, were isolated from CN1. Low but significant nitrogenase activity was detected in all transformants. This is the first example of nitrogenase activity detected in non-diazotrophic photosynthetic organisms. These strains provide valuable platforms to investigate unknown factors that enable nitrogen-fixing growth of non-diazotrophic photosynthetic organisms, including plants.
format	article
author	Ryoma Tsujimoto Hiroya Kotani Konomi Yokomizo Hisanori Yamakawa Aoi Nonaka Yuichi Fujita
author_facet	Ryoma Tsujimoto Hiroya Kotani Konomi Yokomizo Hisanori Yamakawa Aoi Nonaka Yuichi Fujita
author_sort	Ryoma Tsujimoto
title	Functional expression of an oxygen-labile nitrogenase in an oxygenic photosynthetic organism
title_short	Functional expression of an oxygen-labile nitrogenase in an oxygenic photosynthetic organism
title_full	Functional expression of an oxygen-labile nitrogenase in an oxygenic photosynthetic organism
title_fullStr	Functional expression of an oxygen-labile nitrogenase in an oxygenic photosynthetic organism
title_full_unstemmed	Functional expression of an oxygen-labile nitrogenase in an oxygenic photosynthetic organism
title_sort	functional expression of an oxygen-labile nitrogenase in an oxygenic photosynthetic organism
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/8f8bfde25596419d9e28912d38902419
work_keys_str_mv	AT ryomatsujimoto functionalexpressionofanoxygenlabilenitrogenaseinanoxygenicphotosyntheticorganism AT hiroyakotani functionalexpressionofanoxygenlabilenitrogenaseinanoxygenicphotosyntheticorganism AT konomiyokomizo functionalexpressionofanoxygenlabilenitrogenaseinanoxygenicphotosyntheticorganism AT hisanoriyamakawa functionalexpressionofanoxygenlabilenitrogenaseinanoxygenicphotosyntheticorganism AT aoinonaka functionalexpressionofanoxygenlabilenitrogenaseinanoxygenicphotosyntheticorganism AT yuichifujita functionalexpressionofanoxygenlabilenitrogenaseinanoxygenicphotosyntheticorganism
_version_	1718384671255953408

Functional expression of an oxygen-labile nitrogenase in an oxygenic photosynthetic organism

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