A novel cell membrane affinity sample pretreatment technique for recognition and preconcentration of active components from traditional Chinese medicine

Abstract We describe a novel biomembrane affinity sample pretreatment technique to quickly screen and preconcentrate active components from traditional Chinese medicine (TCM), which adopts cell membrane coated silica particles (CMCSPs) as affinity ligands which benefit the biomembrane’s ability to m...

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Autores principales:	Yusi Bu, Xiaoshuang He, Qi Hu, Cheng Wang, Xiaoyu Xie, Sicen Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8f8c62662ed34c0b85056aac2d8d21dc
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spelling	oai:doaj.org-article:8f8c62662ed34c0b85056aac2d8d21dc2021-12-02T11:52:34ZA novel cell membrane affinity sample pretreatment technique for recognition and preconcentration of active components from traditional Chinese medicine10.1038/s41598-017-03709-62045-2322https://doaj.org/article/8f8c62662ed34c0b85056aac2d8d21dc2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03709-6https://doaj.org/toc/2045-2322Abstract We describe a novel biomembrane affinity sample pretreatment technique to quickly screen and preconcentrate active components from traditional Chinese medicine (TCM), which adopts cell membrane coated silica particles (CMCSPs) as affinity ligands which benefit the biomembrane’s ability to maximize simulation of drug-receptor interactions in vivo. In this study, the prepared CMCSPs formed by irreversible adsorption of fibroblast growth factor receptor 4 (FGFR4) cell membrane on the surface of silica were characterized using different spectroscopic and imaging instruments. Drug binding experiments showed the excellent adsorption rate and adsorption capacity of FGFR4/CMCSPs compared with non-coated silica particles. The FGFR4/CMCSPs were used as solid-phase extraction sorbents to pretreat the TCM Aconitum szechenyianum Gay. The resultant FGFR4/CMCSPs exhibited good performance. In addition, high selectivity and recognition ability of the FGFR4/CMCSPs were determined by selectivity experiments. Four alkaloid were screened and identified, one of these alkaloid, napellonine, showed favorable anti-tumor activity in preliminary pharmacological verification trials including cell proliferation and molecular docking assays. The proposed cell membrane affinity sample pretreatment method is a reliable, effective and time-saving method for fast screening and enriching active compounds and can be extended to pretreat other TCMs as leading compounds resources.Yusi BuXiaoshuang HeQi HuCheng WangXiaoyu XieSicen WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yusi Bu Xiaoshuang He Qi Hu Cheng Wang Xiaoyu Xie Sicen Wang A novel cell membrane affinity sample pretreatment technique for recognition and preconcentration of active components from traditional Chinese medicine
description	Abstract We describe a novel biomembrane affinity sample pretreatment technique to quickly screen and preconcentrate active components from traditional Chinese medicine (TCM), which adopts cell membrane coated silica particles (CMCSPs) as affinity ligands which benefit the biomembrane’s ability to maximize simulation of drug-receptor interactions in vivo. In this study, the prepared CMCSPs formed by irreversible adsorption of fibroblast growth factor receptor 4 (FGFR4) cell membrane on the surface of silica were characterized using different spectroscopic and imaging instruments. Drug binding experiments showed the excellent adsorption rate and adsorption capacity of FGFR4/CMCSPs compared with non-coated silica particles. The FGFR4/CMCSPs were used as solid-phase extraction sorbents to pretreat the TCM Aconitum szechenyianum Gay. The resultant FGFR4/CMCSPs exhibited good performance. In addition, high selectivity and recognition ability of the FGFR4/CMCSPs were determined by selectivity experiments. Four alkaloid were screened and identified, one of these alkaloid, napellonine, showed favorable anti-tumor activity in preliminary pharmacological verification trials including cell proliferation and molecular docking assays. The proposed cell membrane affinity sample pretreatment method is a reliable, effective and time-saving method for fast screening and enriching active compounds and can be extended to pretreat other TCMs as leading compounds resources.
format	article
author	Yusi Bu Xiaoshuang He Qi Hu Cheng Wang Xiaoyu Xie Sicen Wang
author_facet	Yusi Bu Xiaoshuang He Qi Hu Cheng Wang Xiaoyu Xie Sicen Wang
author_sort	Yusi Bu
title	A novel cell membrane affinity sample pretreatment technique for recognition and preconcentration of active components from traditional Chinese medicine
title_short	A novel cell membrane affinity sample pretreatment technique for recognition and preconcentration of active components from traditional Chinese medicine
title_full	A novel cell membrane affinity sample pretreatment technique for recognition and preconcentration of active components from traditional Chinese medicine
title_fullStr	A novel cell membrane affinity sample pretreatment technique for recognition and preconcentration of active components from traditional Chinese medicine
title_full_unstemmed	A novel cell membrane affinity sample pretreatment technique for recognition and preconcentration of active components from traditional Chinese medicine
title_sort	novel cell membrane affinity sample pretreatment technique for recognition and preconcentration of active components from traditional chinese medicine
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8f8c62662ed34c0b85056aac2d8d21dc
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_version_	1718394995872890880

A novel cell membrane affinity sample pretreatment technique for recognition and preconcentration of active components from traditional Chinese medicine

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