Diagnostics and genotyping of Canine parvovirus type 2 (CPV-2) from disease cases in south-eastern Poland

Canine parvovirus type 2 is one of the most common causes of death among puppies. Despite preventive vaccination, the disease continues to be diagnosed. The aim of the study was to provide a molecular characterization of CPV-2 isolates found in southeastern Poland. Genetic CPV-2 material was isolate...

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Autores principales:	Kowalczyk Marek, Majer-Dziedzic Barbara, Kostro Krzysztof, Szabelak Aleksandra, Ziętek Jerzy, Gryzinska Magdalena, Jakubczak Andrzej
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2019
Materias:	canine parvovirus type 2 molecular diagnostics phylogenetics vp2 protein Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/8f922cbee6314ce9b7a6be9cf9d832a6
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spelling	oai:doaj.org-article:8f922cbee6314ce9b7a6be9cf9d832a62021-11-17T21:27:53ZDiagnostics and genotyping of Canine parvovirus type 2 (CPV-2) from disease cases in south-eastern Poland1820-744810.2478/acve-2019-0002https://doaj.org/article/8f922cbee6314ce9b7a6be9cf9d832a62019-03-01T00:00:00Zhttps://doi.org/10.2478/acve-2019-0002https://doaj.org/toc/1820-7448Canine parvovirus type 2 is one of the most common causes of death among puppies. Despite preventive vaccination, the disease continues to be diagnosed. The aim of the study was to provide a molecular characterization of CPV-2 isolates found in southeastern Poland. Genetic CPV-2 material was isolated from the blood (n=10) and feces (n=50) of infected dogs. The presence of CPV-2 was confirmed by amplification of sequences coding both VP1 and VP2 protein. The products of the PCR reaction with primers amplifying VP2 protein were sequenced and used for genotyping. Bioinformatics analysis of the sequenced PCR product was performed to determine the phylogenetic relationships with variants recorded in the public databases. Based on the analysis of polymorphism in the nucleotide sequence 7 nucleotide variants were detected and assigned into four amino acid groups. Representatives of three groups contained asparagine at amino acid position 426 of the VP2 protein, which is characteristic of CPV-2a. The variant from the fourth group belonged to type CPV-2b. CPV-2a is the dominant antigenic type of CPV-2 in Poland. The pathogen’s high degree of polymorphism is manifested not only by the presence of numerous variants within the type, but also by the presence of representatives of type CPV-2b. Further studies of the molecular epidemiology of CPV-2 are necessary to optimize the effectiveness of preventive measures.Kowalczyk MarekMajer-Dziedzic BarbaraKostro KrzysztofSzabelak AleksandraZiętek JerzyGryzinska MagdalenaJakubczak AndrzejSciendoarticlecanine parvovirus type 2molecular diagnosticsphylogeneticsvp2 proteinVeterinary medicineSF600-1100ENActa Veterinaria, Vol 69, Iss 1, Pp 32-46 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	canine parvovirus type 2 molecular diagnostics phylogenetics vp2 protein Veterinary medicine SF600-1100
spellingShingle	canine parvovirus type 2 molecular diagnostics phylogenetics vp2 protein Veterinary medicine SF600-1100 Kowalczyk Marek Majer-Dziedzic Barbara Kostro Krzysztof Szabelak Aleksandra Ziętek Jerzy Gryzinska Magdalena Jakubczak Andrzej Diagnostics and genotyping of Canine parvovirus type 2 (CPV-2) from disease cases in south-eastern Poland
description	Canine parvovirus type 2 is one of the most common causes of death among puppies. Despite preventive vaccination, the disease continues to be diagnosed. The aim of the study was to provide a molecular characterization of CPV-2 isolates found in southeastern Poland. Genetic CPV-2 material was isolated from the blood (n=10) and feces (n=50) of infected dogs. The presence of CPV-2 was confirmed by amplification of sequences coding both VP1 and VP2 protein. The products of the PCR reaction with primers amplifying VP2 protein were sequenced and used for genotyping. Bioinformatics analysis of the sequenced PCR product was performed to determine the phylogenetic relationships with variants recorded in the public databases. Based on the analysis of polymorphism in the nucleotide sequence 7 nucleotide variants were detected and assigned into four amino acid groups. Representatives of three groups contained asparagine at amino acid position 426 of the VP2 protein, which is characteristic of CPV-2a. The variant from the fourth group belonged to type CPV-2b. CPV-2a is the dominant antigenic type of CPV-2 in Poland. The pathogen’s high degree of polymorphism is manifested not only by the presence of numerous variants within the type, but also by the presence of representatives of type CPV-2b. Further studies of the molecular epidemiology of CPV-2 are necessary to optimize the effectiveness of preventive measures.
format	article
author	Kowalczyk Marek Majer-Dziedzic Barbara Kostro Krzysztof Szabelak Aleksandra Ziętek Jerzy Gryzinska Magdalena Jakubczak Andrzej
author_facet	Kowalczyk Marek Majer-Dziedzic Barbara Kostro Krzysztof Szabelak Aleksandra Ziętek Jerzy Gryzinska Magdalena Jakubczak Andrzej
author_sort	Kowalczyk Marek
title	Diagnostics and genotyping of Canine parvovirus type 2 (CPV-2) from disease cases in south-eastern Poland
title_short	Diagnostics and genotyping of Canine parvovirus type 2 (CPV-2) from disease cases in south-eastern Poland
title_full	Diagnostics and genotyping of Canine parvovirus type 2 (CPV-2) from disease cases in south-eastern Poland
title_fullStr	Diagnostics and genotyping of Canine parvovirus type 2 (CPV-2) from disease cases in south-eastern Poland
title_full_unstemmed	Diagnostics and genotyping of Canine parvovirus type 2 (CPV-2) from disease cases in south-eastern Poland
title_sort	diagnostics and genotyping of canine parvovirus type 2 (cpv-2) from disease cases in south-eastern poland
publisher	Sciendo
publishDate	2019
url	https://doaj.org/article/8f922cbee6314ce9b7a6be9cf9d832a6
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Diagnostics and genotyping of Canine parvovirus type 2 (CPV-2) from disease cases in south-eastern Poland

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