Expression Signatures of Long Noncoding RNAs in Left Ventricular Noncompaction
Long noncoding RNAs have gained widespread attention in recent years for their crucial role in biological regulation. They have been implicated in a range of developmental processes and diseases including cancer, cardiovascular, and neuronal diseases. However, the role of long noncoding RNAs (lncRNA...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:8f9732307d214b9fbe9ade3caf0615732021-11-10T07:07:52ZExpression Signatures of Long Noncoding RNAs in Left Ventricular Noncompaction2297-055X10.3389/fcvm.2021.763858https://doaj.org/article/8f9732307d214b9fbe9ade3caf0615732021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcvm.2021.763858/fullhttps://doaj.org/toc/2297-055XLong noncoding RNAs have gained widespread attention in recent years for their crucial role in biological regulation. They have been implicated in a range of developmental processes and diseases including cancer, cardiovascular, and neuronal diseases. However, the role of long noncoding RNAs (lncRNAs) in left ventricular noncompaction (LVNC) has not been explored. In this study, we investigated the expression levels of lncRNAs in the blood of LVNC patients and healthy subjects to identify differentially expressed lncRNA that develop LVNC specific biomarkers and targets for developing therapies using biological pathways. We used Agilent Human lncRNA array that contains both updated lncRNAs and mRNAs probes. We identified 1,568 upregulated and 1,141 downregulated (log fold-change > 2.0) lncRNAs that are differentially expressed between LVNC and the control group. Among them, RP11-1100L3.7 and XLOC_002730 are the most upregulated and downregulated lncRNAs. Using quantitative real-time reverse transcription polymerase chain reaction (RT-QPCR), we confirmed the differential expression of three top upregulated and downregulated lncRNAs along with two other randomly picked lncRNAs. Gene Ontology (GO) and KEGG pathways analysis with these differentially expressed lncRNAs provide insight into the cellular pathway leading to LVNC pathogenesis. We also identified 1,066 upregulated and 1,017 downregulated mRNAs. Gene set enrichment analysis (GSEA) showed that G2M, Estrogen, and inflammatory pathways are enriched in differentially expressed genes (DEG). We also identified miRNA targets for these differentially expressed genes. In this study, we first report the use of LncRNA microarray to understand the pathogenesis of LVNC and to identify several lncRNA and genes and their targets as potential biomarkers.Qingshan TianHanxiao NiuHanxiao NiuDingyang LiuDingyang LiuNa TaNa TaQing YangVikram NortonYujing WuYujing WuAmit K. MaitiHao WuZhenzhong ZhengFrontiers Media S.A.articlelncRNAsLVNCmRNAsmiRNA targetsbiomarkerDiseases of the circulatory (Cardiovascular) systemRC666-701ENFrontiers in Cardiovascular Medicine, Vol 8 (2021) |
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DOAJ |
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EN |
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lncRNAs LVNC mRNAs miRNA targets biomarker Diseases of the circulatory (Cardiovascular) system RC666-701 |
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lncRNAs LVNC mRNAs miRNA targets biomarker Diseases of the circulatory (Cardiovascular) system RC666-701 Qingshan Tian Hanxiao Niu Hanxiao Niu Dingyang Liu Dingyang Liu Na Ta Na Ta Qing Yang Vikram Norton Yujing Wu Yujing Wu Amit K. Maiti Hao Wu Zhenzhong Zheng Expression Signatures of Long Noncoding RNAs in Left Ventricular Noncompaction |
| description |
Long noncoding RNAs have gained widespread attention in recent years for their crucial role in biological regulation. They have been implicated in a range of developmental processes and diseases including cancer, cardiovascular, and neuronal diseases. However, the role of long noncoding RNAs (lncRNAs) in left ventricular noncompaction (LVNC) has not been explored. In this study, we investigated the expression levels of lncRNAs in the blood of LVNC patients and healthy subjects to identify differentially expressed lncRNA that develop LVNC specific biomarkers and targets for developing therapies using biological pathways. We used Agilent Human lncRNA array that contains both updated lncRNAs and mRNAs probes. We identified 1,568 upregulated and 1,141 downregulated (log fold-change > 2.0) lncRNAs that are differentially expressed between LVNC and the control group. Among them, RP11-1100L3.7 and XLOC_002730 are the most upregulated and downregulated lncRNAs. Using quantitative real-time reverse transcription polymerase chain reaction (RT-QPCR), we confirmed the differential expression of three top upregulated and downregulated lncRNAs along with two other randomly picked lncRNAs. Gene Ontology (GO) and KEGG pathways analysis with these differentially expressed lncRNAs provide insight into the cellular pathway leading to LVNC pathogenesis. We also identified 1,066 upregulated and 1,017 downregulated mRNAs. Gene set enrichment analysis (GSEA) showed that G2M, Estrogen, and inflammatory pathways are enriched in differentially expressed genes (DEG). We also identified miRNA targets for these differentially expressed genes. In this study, we first report the use of LncRNA microarray to understand the pathogenesis of LVNC and to identify several lncRNA and genes and their targets as potential biomarkers. |
| format |
article |
| author |
Qingshan Tian Hanxiao Niu Hanxiao Niu Dingyang Liu Dingyang Liu Na Ta Na Ta Qing Yang Vikram Norton Yujing Wu Yujing Wu Amit K. Maiti Hao Wu Zhenzhong Zheng |
| author_facet |
Qingshan Tian Hanxiao Niu Hanxiao Niu Dingyang Liu Dingyang Liu Na Ta Na Ta Qing Yang Vikram Norton Yujing Wu Yujing Wu Amit K. Maiti Hao Wu Zhenzhong Zheng |
| author_sort |
Qingshan Tian |
| title |
Expression Signatures of Long Noncoding RNAs in Left Ventricular Noncompaction |
| title_short |
Expression Signatures of Long Noncoding RNAs in Left Ventricular Noncompaction |
| title_full |
Expression Signatures of Long Noncoding RNAs in Left Ventricular Noncompaction |
| title_fullStr |
Expression Signatures of Long Noncoding RNAs in Left Ventricular Noncompaction |
| title_full_unstemmed |
Expression Signatures of Long Noncoding RNAs in Left Ventricular Noncompaction |
| title_sort |
expression signatures of long noncoding rnas in left ventricular noncompaction |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/8f9732307d214b9fbe9ade3caf061573 |
| work_keys_str_mv |
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