De novo identification of essential protein domains from CRISPR-Cas9 tiling-sgRNA knockout screens

Tiling-sgRNA designs allow the in situ evaluation of protein domain functions. Here the authors present ProTiler - a computational method to predict CRISPR knockout hyper-sensitive regions, revealing previously unannotated domains.

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Autores principales:	Wei He, Liang Zhang, Oscar D. Villarreal, Rongjie Fu, Ella Bedford, Jingzhuang Dou, Anish Y. Patel, Mark T. Bedford, Xiaobing Shi, Taiping Chen, Blaine Bartholomew, Han Xu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/8f9bec4646d34e8fa3bf3716402d6050
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spelling	oai:doaj.org-article:8f9bec4646d34e8fa3bf3716402d60502021-12-02T14:35:55ZDe novo identification of essential protein domains from CRISPR-Cas9 tiling-sgRNA knockout screens10.1038/s41467-019-12489-82041-1723https://doaj.org/article/8f9bec4646d34e8fa3bf3716402d60502019-10-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-12489-8https://doaj.org/toc/2041-1723Tiling-sgRNA designs allow the in situ evaluation of protein domain functions. Here the authors present ProTiler - a computational method to predict CRISPR knockout hyper-sensitive regions, revealing previously unannotated domains.Wei HeLiang ZhangOscar D. VillarrealRongjie FuElla BedfordJingzhuang DouAnish Y. PatelMark T. BedfordXiaobing ShiTaiping ChenBlaine BartholomewHan XuNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-10 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Wei He Liang Zhang Oscar D. Villarreal Rongjie Fu Ella Bedford Jingzhuang Dou Anish Y. Patel Mark T. Bedford Xiaobing Shi Taiping Chen Blaine Bartholomew Han Xu De novo identification of essential protein domains from CRISPR-Cas9 tiling-sgRNA knockout screens
description	Tiling-sgRNA designs allow the in situ evaluation of protein domain functions. Here the authors present ProTiler - a computational method to predict CRISPR knockout hyper-sensitive regions, revealing previously unannotated domains.
format	article
author	Wei He Liang Zhang Oscar D. Villarreal Rongjie Fu Ella Bedford Jingzhuang Dou Anish Y. Patel Mark T. Bedford Xiaobing Shi Taiping Chen Blaine Bartholomew Han Xu
author_facet	Wei He Liang Zhang Oscar D. Villarreal Rongjie Fu Ella Bedford Jingzhuang Dou Anish Y. Patel Mark T. Bedford Xiaobing Shi Taiping Chen Blaine Bartholomew Han Xu
author_sort	Wei He
title	De novo identification of essential protein domains from CRISPR-Cas9 tiling-sgRNA knockout screens
title_short	De novo identification of essential protein domains from CRISPR-Cas9 tiling-sgRNA knockout screens
title_full	De novo identification of essential protein domains from CRISPR-Cas9 tiling-sgRNA knockout screens
title_fullStr	De novo identification of essential protein domains from CRISPR-Cas9 tiling-sgRNA knockout screens
title_full_unstemmed	De novo identification of essential protein domains from CRISPR-Cas9 tiling-sgRNA knockout screens
title_sort	de novo identification of essential protein domains from crispr-cas9 tiling-sgrna knockout screens
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/8f9bec4646d34e8fa3bf3716402d6050
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De novo identification of essential protein domains from CRISPR-Cas9 tiling-sgRNA knockout screens

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