Genomic analyses of the microsporidian Nosema ceranae, an emergent pathogen of honey bees.

Recent steep declines in honey bee health have severely impacted the beekeeping industry, presenting new risks for agricultural commodities that depend on insect pollination. Honey bee declines could reflect increased pressures from parasites and pathogens. The incidence of the microsporidian pathog...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	R Scott Cornman, Yan Ping Chen, Michael C Schatz, Craig Street, Yan Zhao, Brian Desany, Michael Egholm, Stephen Hutchison, Jeffery S Pettis, W Ian Lipkin, Jay D Evans
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/8fa6c49fab21462a9e9aa2860c10b81f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8fa6c49fab21462a9e9aa2860c10b81f
record_format	dspace
spelling	oai:doaj.org-article:8fa6c49fab21462a9e9aa2860c10b81f2021-11-25T05:47:51ZGenomic analyses of the microsporidian Nosema ceranae, an emergent pathogen of honey bees.1553-73661553-737410.1371/journal.ppat.1000466https://doaj.org/article/8fa6c49fab21462a9e9aa2860c10b81f2009-06-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19503607/pdf/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Recent steep declines in honey bee health have severely impacted the beekeeping industry, presenting new risks for agricultural commodities that depend on insect pollination. Honey bee declines could reflect increased pressures from parasites and pathogens. The incidence of the microsporidian pathogen Nosema ceranae has increased significantly in the past decade. Here we present a draft assembly (7.86 MB) of the N. ceranae genome derived from pyrosequence data, including initial gene models and genomic comparisons with other members of this highly derived fungal lineage. N. ceranae has a strongly AT-biased genome (74% A+T) and a diversity of repetitive elements, complicating the assembly. Of 2,614 predicted protein-coding sequences, we conservatively estimate that 1,366 have homologs in the microsporidian Encephalitozoon cuniculi, the most closely related published genome sequence. We identify genes conserved among microsporidia that lack clear homology outside this group, which are of special interest as potential virulence factors in this group of obligate parasites. A substantial fraction of the diminutive N. ceranae proteome consists of novel and transposable-element proteins. For a majority of well-supported gene models, a conserved sense-strand motif can be found within 15 bases upstream of the start codon; a previously uncharacterized version of this motif is also present in E. cuniculi. These comparisons provide insight into the architecture, regulation, and evolution of microsporidian genomes, and will drive investigations into honey bee-Nosema interactions.R Scott CornmanYan Ping ChenMichael C SchatzCraig StreetYan ZhaoBrian DesanyMichael EgholmStephen HutchisonJeffery S PettisW Ian LipkinJay D EvansPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 5, Iss 6, p e1000466 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 R Scott Cornman Yan Ping Chen Michael C Schatz Craig Street Yan Zhao Brian Desany Michael Egholm Stephen Hutchison Jeffery S Pettis W Ian Lipkin Jay D Evans Genomic analyses of the microsporidian Nosema ceranae, an emergent pathogen of honey bees.
description	Recent steep declines in honey bee health have severely impacted the beekeeping industry, presenting new risks for agricultural commodities that depend on insect pollination. Honey bee declines could reflect increased pressures from parasites and pathogens. The incidence of the microsporidian pathogen Nosema ceranae has increased significantly in the past decade. Here we present a draft assembly (7.86 MB) of the N. ceranae genome derived from pyrosequence data, including initial gene models and genomic comparisons with other members of this highly derived fungal lineage. N. ceranae has a strongly AT-biased genome (74% A+T) and a diversity of repetitive elements, complicating the assembly. Of 2,614 predicted protein-coding sequences, we conservatively estimate that 1,366 have homologs in the microsporidian Encephalitozoon cuniculi, the most closely related published genome sequence. We identify genes conserved among microsporidia that lack clear homology outside this group, which are of special interest as potential virulence factors in this group of obligate parasites. A substantial fraction of the diminutive N. ceranae proteome consists of novel and transposable-element proteins. For a majority of well-supported gene models, a conserved sense-strand motif can be found within 15 bases upstream of the start codon; a previously uncharacterized version of this motif is also present in E. cuniculi. These comparisons provide insight into the architecture, regulation, and evolution of microsporidian genomes, and will drive investigations into honey bee-Nosema interactions.
format	article
author	R Scott Cornman Yan Ping Chen Michael C Schatz Craig Street Yan Zhao Brian Desany Michael Egholm Stephen Hutchison Jeffery S Pettis W Ian Lipkin Jay D Evans
author_facet	R Scott Cornman Yan Ping Chen Michael C Schatz Craig Street Yan Zhao Brian Desany Michael Egholm Stephen Hutchison Jeffery S Pettis W Ian Lipkin Jay D Evans
author_sort	R Scott Cornman
title	Genomic analyses of the microsporidian Nosema ceranae, an emergent pathogen of honey bees.
title_short	Genomic analyses of the microsporidian Nosema ceranae, an emergent pathogen of honey bees.
title_full	Genomic analyses of the microsporidian Nosema ceranae, an emergent pathogen of honey bees.
title_fullStr	Genomic analyses of the microsporidian Nosema ceranae, an emergent pathogen of honey bees.
title_full_unstemmed	Genomic analyses of the microsporidian Nosema ceranae, an emergent pathogen of honey bees.
title_sort	genomic analyses of the microsporidian nosema ceranae, an emergent pathogen of honey bees.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/8fa6c49fab21462a9e9aa2860c10b81f
work_keys_str_mv	AT rscottcornman genomicanalysesofthemicrosporidiannosemaceranaeanemergentpathogenofhoneybees AT yanpingchen genomicanalysesofthemicrosporidiannosemaceranaeanemergentpathogenofhoneybees AT michaelcschatz genomicanalysesofthemicrosporidiannosemaceranaeanemergentpathogenofhoneybees AT craigstreet genomicanalysesofthemicrosporidiannosemaceranaeanemergentpathogenofhoneybees AT yanzhao genomicanalysesofthemicrosporidiannosemaceranaeanemergentpathogenofhoneybees AT briandesany genomicanalysesofthemicrosporidiannosemaceranaeanemergentpathogenofhoneybees AT michaelegholm genomicanalysesofthemicrosporidiannosemaceranaeanemergentpathogenofhoneybees AT stephenhutchison genomicanalysesofthemicrosporidiannosemaceranaeanemergentpathogenofhoneybees AT jefferyspettis genomicanalysesofthemicrosporidiannosemaceranaeanemergentpathogenofhoneybees AT wianlipkin genomicanalysesofthemicrosporidiannosemaceranaeanemergentpathogenofhoneybees AT jaydevans genomicanalysesofthemicrosporidiannosemaceranaeanemergentpathogenofhoneybees
_version_	1718414470022168576

Genomic analyses of the microsporidian Nosema ceranae, an emergent pathogen of honey bees.

Ejemplares similares