Identification and analysis of brown planthopper-responsive microRNAs in resistant and susceptible rice plants

Abstract The brown planthopper (BPH) is the most devastating insect pest of rice. The rice gene BPH15 confers resistance to BPH. MicroRNAs (miRNAs) regulate a spectrum of development and defense response processes in plants. In this study, we analyzed six miRNA profiles of a BPH15 introgression line...

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Autores principales:	Yan Wu, Wentang Lv, Liang Hu, Weiwei Rao, Ya Zeng, Lili Zhu, Yuqing He, Guangcun He
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8fa918e284974358b365beaa7bb5f528
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spelling	oai:doaj.org-article:8fa918e284974358b365beaa7bb5f5282021-12-02T16:06:17ZIdentification and analysis of brown planthopper-responsive microRNAs in resistant and susceptible rice plants10.1038/s41598-017-09143-y2045-2322https://doaj.org/article/8fa918e284974358b365beaa7bb5f5282017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09143-yhttps://doaj.org/toc/2045-2322Abstract The brown planthopper (BPH) is the most devastating insect pest of rice. The rice gene BPH15 confers resistance to BPH. MicroRNAs (miRNAs) regulate a spectrum of development and defense response processes in plants. In this study, we analyzed six miRNA profiles of a BPH15 introgression line (P15) and a susceptible recipient line (PC) at three time points (0 h, 6 h and 48 h) after BPH attack, and identified 464 known miRNAs and 183 potential novel miRNAs. Before the BPH feeding, we identified 23 miRNAs differentially expressed in P15 and PC. We speculated that the resistant plant is in a priming state by the regulation of miRNAs. After the BPH feeding, 104 miRNAs were found to be expressed differentially in P15 (68 in P15-6/P15-0, 36 in P15-48/P15-0), and 80 miRNAs were found expressed differentially in PC (32 in PC-6/PC-0, 48 in PC-48/PC-0), which illustrated that miRNA expression is activated upon attack. These miRNAs regulate different pathways that contribute to the basal defense and specific resistance of rice to the BPH. Our study provides additional data for scientists to further explore the mechanism of plant defense against insect attack and to find a way for efficient insect control.Yan WuWentang LvLiang HuWeiwei RaoYa ZengLili ZhuYuqing HeGuangcun HeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yan Wu Wentang Lv Liang Hu Weiwei Rao Ya Zeng Lili Zhu Yuqing He Guangcun He Identification and analysis of brown planthopper-responsive microRNAs in resistant and susceptible rice plants
description	Abstract The brown planthopper (BPH) is the most devastating insect pest of rice. The rice gene BPH15 confers resistance to BPH. MicroRNAs (miRNAs) regulate a spectrum of development and defense response processes in plants. In this study, we analyzed six miRNA profiles of a BPH15 introgression line (P15) and a susceptible recipient line (PC) at three time points (0 h, 6 h and 48 h) after BPH attack, and identified 464 known miRNAs and 183 potential novel miRNAs. Before the BPH feeding, we identified 23 miRNAs differentially expressed in P15 and PC. We speculated that the resistant plant is in a priming state by the regulation of miRNAs. After the BPH feeding, 104 miRNAs were found to be expressed differentially in P15 (68 in P15-6/P15-0, 36 in P15-48/P15-0), and 80 miRNAs were found expressed differentially in PC (32 in PC-6/PC-0, 48 in PC-48/PC-0), which illustrated that miRNA expression is activated upon attack. These miRNAs regulate different pathways that contribute to the basal defense and specific resistance of rice to the BPH. Our study provides additional data for scientists to further explore the mechanism of plant defense against insect attack and to find a way for efficient insect control.
format	article
author	Yan Wu Wentang Lv Liang Hu Weiwei Rao Ya Zeng Lili Zhu Yuqing He Guangcun He
author_facet	Yan Wu Wentang Lv Liang Hu Weiwei Rao Ya Zeng Lili Zhu Yuqing He Guangcun He
author_sort	Yan Wu
title	Identification and analysis of brown planthopper-responsive microRNAs in resistant and susceptible rice plants
title_short	Identification and analysis of brown planthopper-responsive microRNAs in resistant and susceptible rice plants
title_full	Identification and analysis of brown planthopper-responsive microRNAs in resistant and susceptible rice plants
title_fullStr	Identification and analysis of brown planthopper-responsive microRNAs in resistant and susceptible rice plants
title_full_unstemmed	Identification and analysis of brown planthopper-responsive microRNAs in resistant and susceptible rice plants
title_sort	identification and analysis of brown planthopper-responsive micrornas in resistant and susceptible rice plants
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8fa918e284974358b365beaa7bb5f528
work_keys_str_mv	AT yanwu identificationandanalysisofbrownplanthopperresponsivemicrornasinresistantandsusceptiblericeplants AT wentanglv identificationandanalysisofbrownplanthopperresponsivemicrornasinresistantandsusceptiblericeplants AT lianghu identificationandanalysisofbrownplanthopperresponsivemicrornasinresistantandsusceptiblericeplants AT weiweirao identificationandanalysisofbrownplanthopperresponsivemicrornasinresistantandsusceptiblericeplants AT yazeng identificationandanalysisofbrownplanthopperresponsivemicrornasinresistantandsusceptiblericeplants AT lilizhu identificationandanalysisofbrownplanthopperresponsivemicrornasinresistantandsusceptiblericeplants AT yuqinghe identificationandanalysisofbrownplanthopperresponsivemicrornasinresistantandsusceptiblericeplants AT guangcunhe identificationandanalysisofbrownplanthopperresponsivemicrornasinresistantandsusceptiblericeplants
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Identification and analysis of brown planthopper-responsive microRNAs in resistant and susceptible rice plants

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