HNRNPA2B1 as a trigger of RNA switch modulates the miRNA-mediated regulation of CDK6

Summary: The functional inactivation of tumor suppressor microRNA (miRNA) is closely related to the tumorigenesis of cancer. There are instances where the miRNA and the corresponding target both exist in a cell, but the target gene silencing do not occur as expected. Herein, we found that both miR-5...

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Autores principales:	Menghui Yin, Meidie Cheng, Chengli Liu, Keli Wu, Wei Xiong, Ji Fang, Yinxiong Li, Biliang Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Biological sciences Biochemistry Molecular biology Cell biology Science Q
Acceso en línea:	https://doaj.org/article/8faa0f143d804ab5b62bfd656793089d
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spelling	oai:doaj.org-article:8faa0f143d804ab5b62bfd656793089d2021-11-20T05:10:15ZHNRNPA2B1 as a trigger of RNA switch modulates the miRNA-mediated regulation of CDK62589-004210.1016/j.isci.2021.103345https://doaj.org/article/8faa0f143d804ab5b62bfd656793089d2021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2589004221013146https://doaj.org/toc/2589-0042Summary: The functional inactivation of tumor suppressor microRNA (miRNA) is closely related to the tumorigenesis of cancer. There are instances where the miRNA and the corresponding target both exist in a cell, but the target gene silencing do not occur as expected. Herein, we found that both miR-506 and its target CDK6 are highly co-expressed in lung cancer cells. Sequence analyses suggested that a miR-506 binding site (1648–1654) and a cis-element (1785–1795) for binding by heterogeneous nuclear ribonucleoprotein A2/B1 (HNRNPA2B1) are evolutionarily conserved and forms a stem structure in the 3′ untranslated region (3′UTR) of CDK6. Furthermore, HNRNPA2B1 can bind to the stem structure to denature it and recruit the RNA helicase DExH-box helicase 9 (DHX9) to the 3′UTR, which ultimately facilitates miRNAs-mediated CDK6 silencing. These results indicate that the cis-element of the 3′UTR of CDK6, where HNRNPA2B1 binds, serves as an RNA switch to regulate miRNAs’ function in cancer cells.Menghui YinMeidie ChengChengli LiuKeli WuWei XiongJi FangYinxiong LiBiliang ZhangElsevierarticleBiological sciencesBiochemistryMolecular biologyCell biologyScienceQENiScience, Vol 24, Iss 11, Pp 103345- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biological sciences Biochemistry Molecular biology Cell biology Science Q
spellingShingle	Biological sciences Biochemistry Molecular biology Cell biology Science Q Menghui Yin Meidie Cheng Chengli Liu Keli Wu Wei Xiong Ji Fang Yinxiong Li Biliang Zhang HNRNPA2B1 as a trigger of RNA switch modulates the miRNA-mediated regulation of CDK6
description	Summary: The functional inactivation of tumor suppressor microRNA (miRNA) is closely related to the tumorigenesis of cancer. There are instances where the miRNA and the corresponding target both exist in a cell, but the target gene silencing do not occur as expected. Herein, we found that both miR-506 and its target CDK6 are highly co-expressed in lung cancer cells. Sequence analyses suggested that a miR-506 binding site (1648–1654) and a cis-element (1785–1795) for binding by heterogeneous nuclear ribonucleoprotein A2/B1 (HNRNPA2B1) are evolutionarily conserved and forms a stem structure in the 3′ untranslated region (3′UTR) of CDK6. Furthermore, HNRNPA2B1 can bind to the stem structure to denature it and recruit the RNA helicase DExH-box helicase 9 (DHX9) to the 3′UTR, which ultimately facilitates miRNAs-mediated CDK6 silencing. These results indicate that the cis-element of the 3′UTR of CDK6, where HNRNPA2B1 binds, serves as an RNA switch to regulate miRNAs’ function in cancer cells.
format	article
author	Menghui Yin Meidie Cheng Chengli Liu Keli Wu Wei Xiong Ji Fang Yinxiong Li Biliang Zhang
author_facet	Menghui Yin Meidie Cheng Chengli Liu Keli Wu Wei Xiong Ji Fang Yinxiong Li Biliang Zhang
author_sort	Menghui Yin
title	HNRNPA2B1 as a trigger of RNA switch modulates the miRNA-mediated regulation of CDK6
title_short	HNRNPA2B1 as a trigger of RNA switch modulates the miRNA-mediated regulation of CDK6
title_full	HNRNPA2B1 as a trigger of RNA switch modulates the miRNA-mediated regulation of CDK6
title_fullStr	HNRNPA2B1 as a trigger of RNA switch modulates the miRNA-mediated regulation of CDK6
title_full_unstemmed	HNRNPA2B1 as a trigger of RNA switch modulates the miRNA-mediated regulation of CDK6
title_sort	hnrnpa2b1 as a trigger of rna switch modulates the mirna-mediated regulation of cdk6
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/8faa0f143d804ab5b62bfd656793089d
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HNRNPA2B1 as a trigger of RNA switch modulates the miRNA-mediated regulation of CDK6

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