Disruption of insect immunity using analogs of the pleiotropic insect peptide hormone Neb-colloostatin: a nanotech approach for pest control II

Abstract This work continues our studies on the pleiotropic activity of the insect peptide Neb-colloostatin in insects. In vivo immunological bioassays demonstrated that hemocytotoxic analogs of Neb-colloostatin injected into Tenebrio molitor significantly reduced the number of hemocytes in the hemo...

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Autores principales:	Patryk Nowicki, Mariola Kuczer, Grzegorz Schroeder, Elżbieta Czarniewska
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8fbc6c9be88140c4b382cd4cd3ef5751
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spelling	oai:doaj.org-article:8fbc6c9be88140c4b382cd4cd3ef57512021-12-02T14:29:09ZDisruption of insect immunity using analogs of the pleiotropic insect peptide hormone Neb-colloostatin: a nanotech approach for pest control II10.1038/s41598-021-87878-52045-2322https://doaj.org/article/8fbc6c9be88140c4b382cd4cd3ef57512021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87878-5https://doaj.org/toc/2045-2322Abstract This work continues our studies on the pleiotropic activity of the insect peptide Neb-colloostatin in insects. In vivo immunological bioassays demonstrated that hemocytotoxic analogs of Neb-colloostatin injected into Tenebrio molitor significantly reduced the number of hemocytes in the hemolymph and impaired phagocytosis, nodulation and phenoloxidase activities in the insects. Among the analogs tested, [Ala1]-,[Val1]-, [Hyp4]- and [Ach4]-colloostatin were particularly potent in disrupting cellular immunity in larvae, pupae and adult insects. This result suggests that the most effective analogs showed increases in the bioactivity period in the hemolymph of insects when compared to Neb-colloostatin. Recently, we demonstrated that it is possible to introduce Neb-colloostatin through the cuticle of an insect into the hemolymph when the peptide is coupled with nanodiamonds. In this study, we showed that [Ala1]-, [Val1]-, [Hyp4]- and [Ach4]-colloostatin, when complexed with nanodiamonds, may also pass through the cuticle into the hemolymph and induce long-term impairments of immunity in T. molitor at all developmental stages. Studies on the tissue selectivity and effectiveness of Neb-colloostatin analogs and efficient methods for their introduction into insects may contribute to the development of eco-friendly pest control methods based on bioactive peptidomimetics.Patryk NowickiMariola KuczerGrzegorz SchroederElżbieta CzarniewskaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Patryk Nowicki Mariola Kuczer Grzegorz Schroeder Elżbieta Czarniewska Disruption of insect immunity using analogs of the pleiotropic insect peptide hormone Neb-colloostatin: a nanotech approach for pest control II
description	Abstract This work continues our studies on the pleiotropic activity of the insect peptide Neb-colloostatin in insects. In vivo immunological bioassays demonstrated that hemocytotoxic analogs of Neb-colloostatin injected into Tenebrio molitor significantly reduced the number of hemocytes in the hemolymph and impaired phagocytosis, nodulation and phenoloxidase activities in the insects. Among the analogs tested, [Ala1]-,[Val1]-, [Hyp4]- and [Ach4]-colloostatin were particularly potent in disrupting cellular immunity in larvae, pupae and adult insects. This result suggests that the most effective analogs showed increases in the bioactivity period in the hemolymph of insects when compared to Neb-colloostatin. Recently, we demonstrated that it is possible to introduce Neb-colloostatin through the cuticle of an insect into the hemolymph when the peptide is coupled with nanodiamonds. In this study, we showed that [Ala1]-, [Val1]-, [Hyp4]- and [Ach4]-colloostatin, when complexed with nanodiamonds, may also pass through the cuticle into the hemolymph and induce long-term impairments of immunity in T. molitor at all developmental stages. Studies on the tissue selectivity and effectiveness of Neb-colloostatin analogs and efficient methods for their introduction into insects may contribute to the development of eco-friendly pest control methods based on bioactive peptidomimetics.
format	article
author	Patryk Nowicki Mariola Kuczer Grzegorz Schroeder Elżbieta Czarniewska
author_facet	Patryk Nowicki Mariola Kuczer Grzegorz Schroeder Elżbieta Czarniewska
author_sort	Patryk Nowicki
title	Disruption of insect immunity using analogs of the pleiotropic insect peptide hormone Neb-colloostatin: a nanotech approach for pest control II
title_short	Disruption of insect immunity using analogs of the pleiotropic insect peptide hormone Neb-colloostatin: a nanotech approach for pest control II
title_full	Disruption of insect immunity using analogs of the pleiotropic insect peptide hormone Neb-colloostatin: a nanotech approach for pest control II
title_fullStr	Disruption of insect immunity using analogs of the pleiotropic insect peptide hormone Neb-colloostatin: a nanotech approach for pest control II
title_full_unstemmed	Disruption of insect immunity using analogs of the pleiotropic insect peptide hormone Neb-colloostatin: a nanotech approach for pest control II
title_sort	disruption of insect immunity using analogs of the pleiotropic insect peptide hormone neb-colloostatin: a nanotech approach for pest control ii
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8fbc6c9be88140c4b382cd4cd3ef5751
work_keys_str_mv	AT patryknowicki disruptionofinsectimmunityusinganalogsofthepleiotropicinsectpeptidehormonenebcolloostatinananotechapproachforpestcontrolii AT mariolakuczer disruptionofinsectimmunityusinganalogsofthepleiotropicinsectpeptidehormonenebcolloostatinananotechapproachforpestcontrolii AT grzegorzschroeder disruptionofinsectimmunityusinganalogsofthepleiotropicinsectpeptidehormonenebcolloostatinananotechapproachforpestcontrolii AT elzbietaczarniewska disruptionofinsectimmunityusinganalogsofthepleiotropicinsectpeptidehormonenebcolloostatinananotechapproachforpestcontrolii
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Disruption of insect immunity using analogs of the pleiotropic insect peptide hormone Neb-colloostatin: a nanotech approach for pest control II

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