Remifentanil attenuates sepsis-induced intestinal injury by inducing autophagy

Remifentanil (RFT), extensively used for general anesthesia, is a synthetic ultra-short-acting opioid used as an anti-inflammatory oxidant to alleviate a plethora of diseases. This study was designed to determine whether RFT would provide protective effects on sepsis-induced intestinal injury. RFT w...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mingli Wang, Shiqi Guo, Yu Zhang, Yao Zhang, Hong Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	remifentanil sepsis-induced intestinal injury autophagy Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/8fc418efd67941af9e0519f3610caac2
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8fc418efd67941af9e0519f3610caac2
record_format	dspace
spelling	oai:doaj.org-article:8fc418efd67941af9e0519f3610caac22021-11-04T15:51:54ZRemifentanil attenuates sepsis-induced intestinal injury by inducing autophagy2165-59792165-598710.1080/21655979.2021.1997562https://doaj.org/article/8fc418efd67941af9e0519f3610caac22021-10-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1997562https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Remifentanil (RFT), extensively used for general anesthesia, is a synthetic ultra-short-acting opioid used as an anti-inflammatory oxidant to alleviate a plethora of diseases. This study was designed to determine whether RFT would provide protective effects on sepsis-induced intestinal injury. RFT was used to incubate the lipopolysaccharide (LPS)-treated IEC-6 cells for determining the role of RFT in sepsis-induced intestinal injury and the underlying mechanism. The determination of cell viability and inflammation of LPS-treated IEC-6 cells influenced by RFT was conducted by Cell counting Kit-8 (CCK-8), RT-qPCR, and western blot, while the detection of LDH, diamine oxidase (DAO), and intestinal-type fatty acid binding proteins (I-FABP) was conducted for determining the intestinal cytotoxicity in these cells. The apoptosis of these cells was detected by TUNEL, with autophagy-related protein expression measured by western blot to confirm whether autophagy was activated. Finally, the aforementioned assays were conducted again after 3-Methyladenine (3-MA), an autophagy inhibitor, was used on these cells to investigate whether RFT exerted its effects on LPS-treated IEC-6 cells via modulation of autophagy. RFT alleviates LPS-induced IEC-6 cell inflammation, cytotoxicity and apoptosis, and autophagy-related proteins were expressed at higher levels when RFT was used on these cells. Nevertheless, further treatment of 3-MA weakened the restorative impacts of RFT on the inflammation, cytotoxicity and apoptosis of these cells. To conclude, this paper is the first to present evidence that RFT attenuates sepsis-induced intestinal injury by inducing autophagy, which will provide instructions for the future investigations into the use of RFT in treatment of intestinal injury.Mingli WangShiqi GuoYu ZhangYao ZhangHong ZhangTaylor & Francis Grouparticleremifentanilsepsis-induced intestinal injuryautophagyBiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	remifentanil sepsis-induced intestinal injury autophagy Biotechnology TP248.13-248.65
spellingShingle	remifentanil sepsis-induced intestinal injury autophagy Biotechnology TP248.13-248.65 Mingli Wang Shiqi Guo Yu Zhang Yao Zhang Hong Zhang Remifentanil attenuates sepsis-induced intestinal injury by inducing autophagy
description	Remifentanil (RFT), extensively used for general anesthesia, is a synthetic ultra-short-acting opioid used as an anti-inflammatory oxidant to alleviate a plethora of diseases. This study was designed to determine whether RFT would provide protective effects on sepsis-induced intestinal injury. RFT was used to incubate the lipopolysaccharide (LPS)-treated IEC-6 cells for determining the role of RFT in sepsis-induced intestinal injury and the underlying mechanism. The determination of cell viability and inflammation of LPS-treated IEC-6 cells influenced by RFT was conducted by Cell counting Kit-8 (CCK-8), RT-qPCR, and western blot, while the detection of LDH, diamine oxidase (DAO), and intestinal-type fatty acid binding proteins (I-FABP) was conducted for determining the intestinal cytotoxicity in these cells. The apoptosis of these cells was detected by TUNEL, with autophagy-related protein expression measured by western blot to confirm whether autophagy was activated. Finally, the aforementioned assays were conducted again after 3-Methyladenine (3-MA), an autophagy inhibitor, was used on these cells to investigate whether RFT exerted its effects on LPS-treated IEC-6 cells via modulation of autophagy. RFT alleviates LPS-induced IEC-6 cell inflammation, cytotoxicity and apoptosis, and autophagy-related proteins were expressed at higher levels when RFT was used on these cells. Nevertheless, further treatment of 3-MA weakened the restorative impacts of RFT on the inflammation, cytotoxicity and apoptosis of these cells. To conclude, this paper is the first to present evidence that RFT attenuates sepsis-induced intestinal injury by inducing autophagy, which will provide instructions for the future investigations into the use of RFT in treatment of intestinal injury.
format	article
author	Mingli Wang Shiqi Guo Yu Zhang Yao Zhang Hong Zhang
author_facet	Mingli Wang Shiqi Guo Yu Zhang Yao Zhang Hong Zhang
author_sort	Mingli Wang
title	Remifentanil attenuates sepsis-induced intestinal injury by inducing autophagy
title_short	Remifentanil attenuates sepsis-induced intestinal injury by inducing autophagy
title_full	Remifentanil attenuates sepsis-induced intestinal injury by inducing autophagy
title_fullStr	Remifentanil attenuates sepsis-induced intestinal injury by inducing autophagy
title_full_unstemmed	Remifentanil attenuates sepsis-induced intestinal injury by inducing autophagy
title_sort	remifentanil attenuates sepsis-induced intestinal injury by inducing autophagy
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/8fc418efd67941af9e0519f3610caac2
work_keys_str_mv	AT mingliwang remifentanilattenuatessepsisinducedintestinalinjurybyinducingautophagy AT shiqiguo remifentanilattenuatessepsisinducedintestinalinjurybyinducingautophagy AT yuzhang remifentanilattenuatessepsisinducedintestinalinjurybyinducingautophagy AT yaozhang remifentanilattenuatessepsisinducedintestinalinjurybyinducingautophagy AT hongzhang remifentanilattenuatessepsisinducedintestinalinjurybyinducingautophagy
_version_	1718444726876635136

Remifentanil attenuates sepsis-induced intestinal injury by inducing autophagy

Ejemplares similares