Modification of the surface of titanium with multifunctional chimeric peptides to prevent biofilm formation via inhibition of initial colonizers

Xi Zhang,1 Hongjuan Geng,1 Lei Gong,2 Qian Zhang,1 Hongjie Li,1 Xu Zhang,1 Yonglan Wang,1 Ping Gao1 1School and Hospital of Stomatology, Tianjin Medical University, Tianjin 300070, China; 2Department of Esophageal Cancer, Tianjin’s Clinical Research Center for Cancer and Key Laboratory of...

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Autores principales:	Zhang X, Geng H, Gong L, Zhang Q, Li H, Wang Y, Gao P
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	multifunctional chimeric peptide modification of titanium surface human β-defensin-3 Ti-binding peptide-1 arginine-glycine-aspartic acid-containing peptides Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/8fc917c1f39e4c6aad7ae907c1a673c9
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spelling	oai:doaj.org-article:8fc917c1f39e4c6aad7ae907c1a673c92021-12-02T07:37:18ZModification of the surface of titanium with multifunctional chimeric peptides to prevent biofilm formation via inhibition of initial colonizers1178-2013https://doaj.org/article/8fc917c1f39e4c6aad7ae907c1a673c92018-09-01T00:00:00Zhttps://www.dovepress.com/modification-of-the-surface-of-titanium-with-multifunctional-chimeric--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Xi Zhang,1 Hongjuan Geng,1 Lei Gong,2 Qian Zhang,1 Hongjie Li,1 Xu Zhang,1 Yonglan Wang,1 Ping Gao1 1School and Hospital of Stomatology, Tianjin Medical University, Tianjin 300070, China; 2Department of Esophageal Cancer, Tianjin’s Clinical Research Center for Cancer and Key Laboratory of Cancer Prevention and Therapy, National Clinical Research Center for Cancer, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300070, China Background: Prevention of bacterial colonization remains a major challenge in the field of oral implant devices. Chimeric peptides with binding, antimicrobial, and osteogenesis motifs may provide a promising alternative for the inhibition of biofilm formation on titanium (Ti) surfaces. Methods: In this study, chimeric peptides were designed by connecting an antimicrobial sequence from human β-defensin-3 with a Ti-binding sequence and arginine-glycine-aspartic acid using a glycine-glycine-glycine linker. Binding to the Ti substrate and antimicrobial properties against streptococci were evaluated. Significant improvement in reduction of bacterial colonization onto the Ti surface was observed, with or without the presence of saliva or serum. The MC3T3-E1 cells grew well on the modified Ti surfaces compared with the control group. Results: The data showed that the three peptide functional motifs maintained their respective functions, and that the antibiofilm mechanism of the chimeric peptide was via suppression of sspA and sspB gene expression. Conclusion: These results indicated that the endogenous peptide fragments engineered on the Ti surface could provide an environmentally friendly approach for improving the biocompatibility of oral implants. Keywords: multifunctional chimeric peptide, modification of titanium surface, human β-defensin-3, Ti-binding peptide-1, arginine-glycine-aspartic acid-containing peptidesZhang XGeng HGong LZhang QLi HZhang XWang YGao PDove Medical Pressarticlemultifunctional chimeric peptidemodification of titanium surfacehuman β-defensin-3Ti-binding peptide-1arginine-glycine-aspartic acid-containing peptidesMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 5361-5375 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	multifunctional chimeric peptide modification of titanium surface human β-defensin-3 Ti-binding peptide-1 arginine-glycine-aspartic acid-containing peptides Medicine (General) R5-920
spellingShingle	multifunctional chimeric peptide modification of titanium surface human β-defensin-3 Ti-binding peptide-1 arginine-glycine-aspartic acid-containing peptides Medicine (General) R5-920 Zhang X Geng H Gong L Zhang Q Li H Zhang X Wang Y Gao P Modification of the surface of titanium with multifunctional chimeric peptides to prevent biofilm formation via inhibition of initial colonizers
description	Xi Zhang,1 Hongjuan Geng,1 Lei Gong,2 Qian Zhang,1 Hongjie Li,1 Xu Zhang,1 Yonglan Wang,1 Ping Gao1 1School and Hospital of Stomatology, Tianjin Medical University, Tianjin 300070, China; 2Department of Esophageal Cancer, Tianjin’s Clinical Research Center for Cancer and Key Laboratory of Cancer Prevention and Therapy, National Clinical Research Center for Cancer, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300070, China Background: Prevention of bacterial colonization remains a major challenge in the field of oral implant devices. Chimeric peptides with binding, antimicrobial, and osteogenesis motifs may provide a promising alternative for the inhibition of biofilm formation on titanium (Ti) surfaces. Methods: In this study, chimeric peptides were designed by connecting an antimicrobial sequence from human β-defensin-3 with a Ti-binding sequence and arginine-glycine-aspartic acid using a glycine-glycine-glycine linker. Binding to the Ti substrate and antimicrobial properties against streptococci were evaluated. Significant improvement in reduction of bacterial colonization onto the Ti surface was observed, with or without the presence of saliva or serum. The MC3T3-E1 cells grew well on the modified Ti surfaces compared with the control group. Results: The data showed that the three peptide functional motifs maintained their respective functions, and that the antibiofilm mechanism of the chimeric peptide was via suppression of sspA and sspB gene expression. Conclusion: These results indicated that the endogenous peptide fragments engineered on the Ti surface could provide an environmentally friendly approach for improving the biocompatibility of oral implants. Keywords: multifunctional chimeric peptide, modification of titanium surface, human β-defensin-3, Ti-binding peptide-1, arginine-glycine-aspartic acid-containing peptides
format	article
author	Zhang X Geng H Gong L Zhang Q Li H Zhang X Wang Y Gao P
author_facet	Zhang X Geng H Gong L Zhang Q Li H Zhang X Wang Y Gao P
author_sort	Zhang X
title	Modification of the surface of titanium with multifunctional chimeric peptides to prevent biofilm formation via inhibition of initial colonizers
title_short	Modification of the surface of titanium with multifunctional chimeric peptides to prevent biofilm formation via inhibition of initial colonizers
title_full	Modification of the surface of titanium with multifunctional chimeric peptides to prevent biofilm formation via inhibition of initial colonizers
title_fullStr	Modification of the surface of titanium with multifunctional chimeric peptides to prevent biofilm formation via inhibition of initial colonizers
title_full_unstemmed	Modification of the surface of titanium with multifunctional chimeric peptides to prevent biofilm formation via inhibition of initial colonizers
title_sort	modification of the surface of titanium with multifunctional chimeric peptides to prevent biofilm formation via inhibition of initial colonizers
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/8fc917c1f39e4c6aad7ae907c1a673c9
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Modification of the surface of titanium with multifunctional chimeric peptides to prevent biofilm formation via inhibition of initial colonizers

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