PmAP2-β depletion enhanced activation of the Toll signaling pathway during yellow head virus infection in the black tiger shrimp Penaeus monodon

Abstract Yellow head virus (YHV) is a pathogen which causes high mortality in penaeid shrimp. Previous studies suggested that YHV enters shrimp cells via clathrin-mediated endocytosis. This research investigated the roles of clathrin adaptor protein 2 subunit β (AP-2β) from Penaeus monodon during YH...

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Autores principales:	Thapanan Jatuyosporn, Pasunee Laohawutthichai, Premruethai Supungul, Rogerio R. Sotelo-Mundo, Adrian Ochoa-Leyva, Anchalee Tassanakajon, Kuakarun Krusong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8fcb2ceb70724e768ca80bb7361e13ff
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spelling	oai:doaj.org-article:8fcb2ceb70724e768ca80bb7361e13ff2021-12-02T14:59:09ZPmAP2-β depletion enhanced activation of the Toll signaling pathway during yellow head virus infection in the black tiger shrimp Penaeus monodon10.1038/s41598-021-89922-w2045-2322https://doaj.org/article/8fcb2ceb70724e768ca80bb7361e13ff2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89922-whttps://doaj.org/toc/2045-2322Abstract Yellow head virus (YHV) is a pathogen which causes high mortality in penaeid shrimp. Previous studies suggested that YHV enters shrimp cells via clathrin-mediated endocytosis. This research investigated the roles of clathrin adaptor protein 2 subunit β (AP-2β) from Penaeus monodon during YHV infection. PmAP2-β was continuously up-regulated more than twofold during 6–36 hpi. Suppression of PmAP2-β significantly reduced YHV copy numbers and delayed shrimp mortality. Quantitative RT-PCR revealed that knockdown of PmAP2-β significantly enhanced the expression level of PmSpätzle, a signaling ligand in the Toll pathway, by 30-fold at 6 and 12 hpi. Moreover, the expression levels of gene components in the Imd and JAK/STAT signaling pathways under the suppression of PmAP2-β during YHV infection were also investigated. Interestingly, anti-lipopolysaccharide factor isoform 3 (ALFPm3) was up-regulated by 40-fold in PmAP2-β knockdown shrimp upon YHV infection. In addition, silencing of PmAP2-β dramatically enhanced crustinPm1 expression in YHV-infected shrimp. Knockdown of ALFPm3 and crustinPm1 significantly reduced shrimp survival rate. Taken together, this work suggested that PmAP2-β-deficiency promoted the Toll pathway signalings, resulting in elevated levels of ALFPm3 and crustinPm1, the crucial antimicrobial peptides in defence against YHV.Thapanan JatuyospornPasunee LaohawutthichaiPremruethai SupungulRogerio R. Sotelo-MundoAdrian Ochoa-LeyvaAnchalee TassanakajonKuakarun KrusongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Thapanan Jatuyosporn Pasunee Laohawutthichai Premruethai Supungul Rogerio R. Sotelo-Mundo Adrian Ochoa-Leyva Anchalee Tassanakajon Kuakarun Krusong PmAP2-β depletion enhanced activation of the Toll signaling pathway during yellow head virus infection in the black tiger shrimp Penaeus monodon
description	Abstract Yellow head virus (YHV) is a pathogen which causes high mortality in penaeid shrimp. Previous studies suggested that YHV enters shrimp cells via clathrin-mediated endocytosis. This research investigated the roles of clathrin adaptor protein 2 subunit β (AP-2β) from Penaeus monodon during YHV infection. PmAP2-β was continuously up-regulated more than twofold during 6–36 hpi. Suppression of PmAP2-β significantly reduced YHV copy numbers and delayed shrimp mortality. Quantitative RT-PCR revealed that knockdown of PmAP2-β significantly enhanced the expression level of PmSpätzle, a signaling ligand in the Toll pathway, by 30-fold at 6 and 12 hpi. Moreover, the expression levels of gene components in the Imd and JAK/STAT signaling pathways under the suppression of PmAP2-β during YHV infection were also investigated. Interestingly, anti-lipopolysaccharide factor isoform 3 (ALFPm3) was up-regulated by 40-fold in PmAP2-β knockdown shrimp upon YHV infection. In addition, silencing of PmAP2-β dramatically enhanced crustinPm1 expression in YHV-infected shrimp. Knockdown of ALFPm3 and crustinPm1 significantly reduced shrimp survival rate. Taken together, this work suggested that PmAP2-β-deficiency promoted the Toll pathway signalings, resulting in elevated levels of ALFPm3 and crustinPm1, the crucial antimicrobial peptides in defence against YHV.
format	article
author	Thapanan Jatuyosporn Pasunee Laohawutthichai Premruethai Supungul Rogerio R. Sotelo-Mundo Adrian Ochoa-Leyva Anchalee Tassanakajon Kuakarun Krusong
author_facet	Thapanan Jatuyosporn Pasunee Laohawutthichai Premruethai Supungul Rogerio R. Sotelo-Mundo Adrian Ochoa-Leyva Anchalee Tassanakajon Kuakarun Krusong
author_sort	Thapanan Jatuyosporn
title	PmAP2-β depletion enhanced activation of the Toll signaling pathway during yellow head virus infection in the black tiger shrimp Penaeus monodon
title_short	PmAP2-β depletion enhanced activation of the Toll signaling pathway during yellow head virus infection in the black tiger shrimp Penaeus monodon
title_full	PmAP2-β depletion enhanced activation of the Toll signaling pathway during yellow head virus infection in the black tiger shrimp Penaeus monodon
title_fullStr	PmAP2-β depletion enhanced activation of the Toll signaling pathway during yellow head virus infection in the black tiger shrimp Penaeus monodon
title_full_unstemmed	PmAP2-β depletion enhanced activation of the Toll signaling pathway during yellow head virus infection in the black tiger shrimp Penaeus monodon
title_sort	pmap2-β depletion enhanced activation of the toll signaling pathway during yellow head virus infection in the black tiger shrimp penaeus monodon
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8fcb2ceb70724e768ca80bb7361e13ff
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PmAP2-β depletion enhanced activation of the Toll signaling pathway during yellow head virus infection in the black tiger shrimp Penaeus monodon

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