Exercise Training Attenuates Ovariectomy-Induced Alterations in Skeletal Muscle Remodeling, Apoptotic Signaling, and Atrophy Signaling in Rat Skeletal Muscle

Purpose The effects of aerobic exercise training on soleus muscle morphology, mitochondria-mediated apoptotic signaling, and atrophy/hypertrophy signaling in ovariectomized rat skeletal muscle were investigated. Methods Female Sprague-Dawley rats were divided into control (CON), ovariectomy (OVX), a...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Eun-Jeong Cho, Youngju Choi, Jiyeon Kim, Jun Hyun Bae, Jinkyung Cho, Dong-Ho Park, Ju-Hee Kang, Jin Hwan Yoon, Eunmi Park, Dae Yun Seo, Sukho Lee, Hyo-Bum Kwak
Formato: article
Lenguaje:EN
Publicado: Korean Continence Society 2021
Materias:
Acceso en línea:https://doaj.org/article/8fced9389d924c49956d708c74ff0692
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:8fced9389d924c49956d708c74ff0692
record_format dspace
spelling oai:doaj.org-article:8fced9389d924c49956d708c74ff06922021-11-30T04:10:47ZExercise Training Attenuates Ovariectomy-Induced Alterations in Skeletal Muscle Remodeling, Apoptotic Signaling, and Atrophy Signaling in Rat Skeletal Muscle2093-47772093-693110.5213/inj.2142334.167https://doaj.org/article/8fced9389d924c49956d708c74ff06922021-11-01T00:00:00Zhttp://www.einj.org/upload/pdf/inj-2142334-167.pdfhttps://doaj.org/toc/2093-4777https://doaj.org/toc/2093-6931Purpose The effects of aerobic exercise training on soleus muscle morphology, mitochondria-mediated apoptotic signaling, and atrophy/hypertrophy signaling in ovariectomized rat skeletal muscle were investigated. Methods Female Sprague-Dawley rats were divided into control (CON), ovariectomy (OVX), and ovariectomy plus exercise (OVX+EX) groups. After ovarian excision, exercise training was performed using a rat treadmill at 20 m/min, 50 min/day, 5 days/week for 12 weeks. Protein levels of mitochondria-mediated apoptotic signaling and atrophy/hypertrophy signaling in the skeletal muscle (soleus) were examined through western immunoblot analysis. Results The number of myocytes and myocyte cross-sectional area (CSA) were increased and the extramyocyte space was decreased in the OVX group compared to those in the CON group. However, aerobic exercise training significantly increased myocyte CSA and decreased extramyocyte space in the OVX+EX group compared to those in the OVX group. The protein levels of proapoptotic signaling and muscle atrophy signaling were significantly increased, whereas the protein levels of muscle hypertrophy signaling were significantly decreased in the OVX group compared to that in the CON group. Aerobic exercise training significantly decreased the protein levels of proapoptotic signaling and increased the protein level of antiapoptotic protein in the OVX+EX group compared to that in the OVX group. Aerobic exercise training significantly increased the protein levels of hypertrophy signaling and decreased protein levels of atrophy signaling in the OVX+EX group compared to those in the OVX group. Conclusions Treadmill exercise improved estrogen deficiency-induced impairment in skeletal muscle remodeling, mitochondria-mediated apoptotic signaling, and atrophy/hypertrophy signaling in skeletal muscle.Eun-Jeong ChoYoungju ChoiJiyeon KimJun Hyun BaeJinkyung ChoDong-Ho ParkJu-Hee KangJin Hwan YoonEunmi ParkDae Yun SeoSukho LeeHyo-Bum KwakKorean Continence Societyarticleestrogenovariectomysarcopeniaexerciseskeletal muscleDiseases of the genitourinary system. UrologyRC870-923ENInternational Neurourology Journal, Vol 25, Iss Suppl 2, Pp S47-54 (2021)
institution DOAJ
collection DOAJ
language EN
topic estrogen
ovariectomy
sarcopenia
exercise
skeletal muscle
Diseases of the genitourinary system. Urology
RC870-923
spellingShingle estrogen
ovariectomy
sarcopenia
exercise
skeletal muscle
Diseases of the genitourinary system. Urology
RC870-923
Eun-Jeong Cho
Youngju Choi
Jiyeon Kim
Jun Hyun Bae
Jinkyung Cho
Dong-Ho Park
Ju-Hee Kang
Jin Hwan Yoon
Eunmi Park
Dae Yun Seo
Sukho Lee
Hyo-Bum Kwak
Exercise Training Attenuates Ovariectomy-Induced Alterations in Skeletal Muscle Remodeling, Apoptotic Signaling, and Atrophy Signaling in Rat Skeletal Muscle
description Purpose The effects of aerobic exercise training on soleus muscle morphology, mitochondria-mediated apoptotic signaling, and atrophy/hypertrophy signaling in ovariectomized rat skeletal muscle were investigated. Methods Female Sprague-Dawley rats were divided into control (CON), ovariectomy (OVX), and ovariectomy plus exercise (OVX+EX) groups. After ovarian excision, exercise training was performed using a rat treadmill at 20 m/min, 50 min/day, 5 days/week for 12 weeks. Protein levels of mitochondria-mediated apoptotic signaling and atrophy/hypertrophy signaling in the skeletal muscle (soleus) were examined through western immunoblot analysis. Results The number of myocytes and myocyte cross-sectional area (CSA) were increased and the extramyocyte space was decreased in the OVX group compared to those in the CON group. However, aerobic exercise training significantly increased myocyte CSA and decreased extramyocyte space in the OVX+EX group compared to those in the OVX group. The protein levels of proapoptotic signaling and muscle atrophy signaling were significantly increased, whereas the protein levels of muscle hypertrophy signaling were significantly decreased in the OVX group compared to that in the CON group. Aerobic exercise training significantly decreased the protein levels of proapoptotic signaling and increased the protein level of antiapoptotic protein in the OVX+EX group compared to that in the OVX group. Aerobic exercise training significantly increased the protein levels of hypertrophy signaling and decreased protein levels of atrophy signaling in the OVX+EX group compared to those in the OVX group. Conclusions Treadmill exercise improved estrogen deficiency-induced impairment in skeletal muscle remodeling, mitochondria-mediated apoptotic signaling, and atrophy/hypertrophy signaling in skeletal muscle.
format article
author Eun-Jeong Cho
Youngju Choi
Jiyeon Kim
Jun Hyun Bae
Jinkyung Cho
Dong-Ho Park
Ju-Hee Kang
Jin Hwan Yoon
Eunmi Park
Dae Yun Seo
Sukho Lee
Hyo-Bum Kwak
author_facet Eun-Jeong Cho
Youngju Choi
Jiyeon Kim
Jun Hyun Bae
Jinkyung Cho
Dong-Ho Park
Ju-Hee Kang
Jin Hwan Yoon
Eunmi Park
Dae Yun Seo
Sukho Lee
Hyo-Bum Kwak
author_sort Eun-Jeong Cho
title Exercise Training Attenuates Ovariectomy-Induced Alterations in Skeletal Muscle Remodeling, Apoptotic Signaling, and Atrophy Signaling in Rat Skeletal Muscle
title_short Exercise Training Attenuates Ovariectomy-Induced Alterations in Skeletal Muscle Remodeling, Apoptotic Signaling, and Atrophy Signaling in Rat Skeletal Muscle
title_full Exercise Training Attenuates Ovariectomy-Induced Alterations in Skeletal Muscle Remodeling, Apoptotic Signaling, and Atrophy Signaling in Rat Skeletal Muscle
title_fullStr Exercise Training Attenuates Ovariectomy-Induced Alterations in Skeletal Muscle Remodeling, Apoptotic Signaling, and Atrophy Signaling in Rat Skeletal Muscle
title_full_unstemmed Exercise Training Attenuates Ovariectomy-Induced Alterations in Skeletal Muscle Remodeling, Apoptotic Signaling, and Atrophy Signaling in Rat Skeletal Muscle
title_sort exercise training attenuates ovariectomy-induced alterations in skeletal muscle remodeling, apoptotic signaling, and atrophy signaling in rat skeletal muscle
publisher Korean Continence Society
publishDate 2021
url https://doaj.org/article/8fced9389d924c49956d708c74ff0692
work_keys_str_mv AT eunjeongcho exercisetrainingattenuatesovariectomyinducedalterationsinskeletalmuscleremodelingapoptoticsignalingandatrophysignalinginratskeletalmuscle
AT youngjuchoi exercisetrainingattenuatesovariectomyinducedalterationsinskeletalmuscleremodelingapoptoticsignalingandatrophysignalinginratskeletalmuscle
AT jiyeonkim exercisetrainingattenuatesovariectomyinducedalterationsinskeletalmuscleremodelingapoptoticsignalingandatrophysignalinginratskeletalmuscle
AT junhyunbae exercisetrainingattenuatesovariectomyinducedalterationsinskeletalmuscleremodelingapoptoticsignalingandatrophysignalinginratskeletalmuscle
AT jinkyungcho exercisetrainingattenuatesovariectomyinducedalterationsinskeletalmuscleremodelingapoptoticsignalingandatrophysignalinginratskeletalmuscle
AT donghopark exercisetrainingattenuatesovariectomyinducedalterationsinskeletalmuscleremodelingapoptoticsignalingandatrophysignalinginratskeletalmuscle
AT juheekang exercisetrainingattenuatesovariectomyinducedalterationsinskeletalmuscleremodelingapoptoticsignalingandatrophysignalinginratskeletalmuscle
AT jinhwanyoon exercisetrainingattenuatesovariectomyinducedalterationsinskeletalmuscleremodelingapoptoticsignalingandatrophysignalinginratskeletalmuscle
AT eunmipark exercisetrainingattenuatesovariectomyinducedalterationsinskeletalmuscleremodelingapoptoticsignalingandatrophysignalinginratskeletalmuscle
AT daeyunseo exercisetrainingattenuatesovariectomyinducedalterationsinskeletalmuscleremodelingapoptoticsignalingandatrophysignalinginratskeletalmuscle
AT sukholee exercisetrainingattenuatesovariectomyinducedalterationsinskeletalmuscleremodelingapoptoticsignalingandatrophysignalinginratskeletalmuscle
AT hyobumkwak exercisetrainingattenuatesovariectomyinducedalterationsinskeletalmuscleremodelingapoptoticsignalingandatrophysignalinginratskeletalmuscle
_version_ 1718406875432615936