The Irish potato famine pathogen Phytophthora infestans translocates the CRN8 kinase into host plant cells.

Phytopathogenic oomycetes, such as Phytophthora infestans, secrete an arsenal of effector proteins that modulate plant innate immunity to enable infection. We describe CRN8, a host-translocated effector of P. infestans that has kinase activity in planta. CRN8 is a modular protein of the CRN effector...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mireille van Damme, Tolga O Bozkurt, Cahid Cakir, Sebastian Schornack, Jan Sklenar, Alexandra M E Jones, Sophien Kamoun
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/8fd5c14c9fe644208d53fe2e766bf61d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8fd5c14c9fe644208d53fe2e766bf61d
record_format	dspace
spelling	oai:doaj.org-article:8fd5c14c9fe644208d53fe2e766bf61d2021-11-18T06:04:03ZThe Irish potato famine pathogen Phytophthora infestans translocates the CRN8 kinase into host plant cells.1553-73661553-737410.1371/journal.ppat.1002875https://doaj.org/article/8fd5c14c9fe644208d53fe2e766bf61d2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22927814/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Phytopathogenic oomycetes, such as Phytophthora infestans, secrete an arsenal of effector proteins that modulate plant innate immunity to enable infection. We describe CRN8, a host-translocated effector of P. infestans that has kinase activity in planta. CRN8 is a modular protein of the CRN effector family. The C-terminus of CRN8 localizes to the host nucleus and triggers cell death when the protein is expressed in planta. Cell death induction by CRN8 is dependent on its localization to the plant nucleus, which requires a functional nuclear localization signal (NLS). The C-terminal sequence of CRN8 has similarity to a serine/threonine RD kinase domain. We demonstrated that CRN8 is a functional RD kinase and that its auto-phosphorylation is dependent on an intact catalytic site. Co-immunoprecipitation experiments revealed that CRN8 forms a dimer or multimer. Heterologous expression of CRN8 in planta resulted in enhanced virulence by P. infestans. In contrast, in planta expression of the dominant-negative CRN8(R469A;D470A) resulted in reduced P. infestans infection, further implicating CRN8 in virulence. Overall, our results indicate that similar to animal parasites, plant pathogens also translocate biochemically active kinase effectors inside host cells.Mireille van DammeTolga O BozkurtCahid CakirSebastian SchornackJan SklenarAlexandra M E JonesSophien KamounPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 8, Iss 8, p e1002875 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Mireille van Damme Tolga O Bozkurt Cahid Cakir Sebastian Schornack Jan Sklenar Alexandra M E Jones Sophien Kamoun The Irish potato famine pathogen Phytophthora infestans translocates the CRN8 kinase into host plant cells.
description	Phytopathogenic oomycetes, such as Phytophthora infestans, secrete an arsenal of effector proteins that modulate plant innate immunity to enable infection. We describe CRN8, a host-translocated effector of P. infestans that has kinase activity in planta. CRN8 is a modular protein of the CRN effector family. The C-terminus of CRN8 localizes to the host nucleus and triggers cell death when the protein is expressed in planta. Cell death induction by CRN8 is dependent on its localization to the plant nucleus, which requires a functional nuclear localization signal (NLS). The C-terminal sequence of CRN8 has similarity to a serine/threonine RD kinase domain. We demonstrated that CRN8 is a functional RD kinase and that its auto-phosphorylation is dependent on an intact catalytic site. Co-immunoprecipitation experiments revealed that CRN8 forms a dimer or multimer. Heterologous expression of CRN8 in planta resulted in enhanced virulence by P. infestans. In contrast, in planta expression of the dominant-negative CRN8(R469A;D470A) resulted in reduced P. infestans infection, further implicating CRN8 in virulence. Overall, our results indicate that similar to animal parasites, plant pathogens also translocate biochemically active kinase effectors inside host cells.
format	article
author	Mireille van Damme Tolga O Bozkurt Cahid Cakir Sebastian Schornack Jan Sklenar Alexandra M E Jones Sophien Kamoun
author_facet	Mireille van Damme Tolga O Bozkurt Cahid Cakir Sebastian Schornack Jan Sklenar Alexandra M E Jones Sophien Kamoun
author_sort	Mireille van Damme
title	The Irish potato famine pathogen Phytophthora infestans translocates the CRN8 kinase into host plant cells.
title_short	The Irish potato famine pathogen Phytophthora infestans translocates the CRN8 kinase into host plant cells.
title_full	The Irish potato famine pathogen Phytophthora infestans translocates the CRN8 kinase into host plant cells.
title_fullStr	The Irish potato famine pathogen Phytophthora infestans translocates the CRN8 kinase into host plant cells.
title_full_unstemmed	The Irish potato famine pathogen Phytophthora infestans translocates the CRN8 kinase into host plant cells.
title_sort	irish potato famine pathogen phytophthora infestans translocates the crn8 kinase into host plant cells.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/8fd5c14c9fe644208d53fe2e766bf61d
work_keys_str_mv	AT mireillevandamme theirishpotatofaminepathogenphytophthorainfestanstranslocatesthecrn8kinaseintohostplantcells AT tolgaobozkurt theirishpotatofaminepathogenphytophthorainfestanstranslocatesthecrn8kinaseintohostplantcells AT cahidcakir theirishpotatofaminepathogenphytophthorainfestanstranslocatesthecrn8kinaseintohostplantcells AT sebastianschornack theirishpotatofaminepathogenphytophthorainfestanstranslocatesthecrn8kinaseintohostplantcells AT jansklenar theirishpotatofaminepathogenphytophthorainfestanstranslocatesthecrn8kinaseintohostplantcells AT alexandramejones theirishpotatofaminepathogenphytophthorainfestanstranslocatesthecrn8kinaseintohostplantcells AT sophienkamoun theirishpotatofaminepathogenphytophthorainfestanstranslocatesthecrn8kinaseintohostplantcells AT mireillevandamme irishpotatofaminepathogenphytophthorainfestanstranslocatesthecrn8kinaseintohostplantcells AT tolgaobozkurt irishpotatofaminepathogenphytophthorainfestanstranslocatesthecrn8kinaseintohostplantcells AT cahidcakir irishpotatofaminepathogenphytophthorainfestanstranslocatesthecrn8kinaseintohostplantcells AT sebastianschornack irishpotatofaminepathogenphytophthorainfestanstranslocatesthecrn8kinaseintohostplantcells AT jansklenar irishpotatofaminepathogenphytophthorainfestanstranslocatesthecrn8kinaseintohostplantcells AT alexandramejones irishpotatofaminepathogenphytophthorainfestanstranslocatesthecrn8kinaseintohostplantcells AT sophienkamoun irishpotatofaminepathogenphytophthorainfestanstranslocatesthecrn8kinaseintohostplantcells
_version_	1718424617333293056

The Irish potato famine pathogen Phytophthora infestans translocates the CRN8 kinase into host plant cells.

Ejemplares similares