Thermoresponsive GenisteinNLC-dexamethasone-moxifloxacin multi drug delivery system in lens capsule bag to prevent complications after cataract surgery

Abstract Cataract surgery is the most common intraocular procedure. To decrease postsurgical inflammation, prevent infection and reduce the incidence of secondary cataract, we built a temperature-sensitive drug delivery system carrying dexamethasone, moxifloxacin and genistein nanostructured lipid c...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Tingyu Yan, Zhongxu Ma, Jingjing Liu, Na Yin, Shizhen Lei, Xinxin Zhang, Xuedong Li, Yu Zhang, Jun Kong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8fde6917f8d6461faaaf59ecc4eeb315
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8fde6917f8d6461faaaf59ecc4eeb315
record_format	dspace
spelling	oai:doaj.org-article:8fde6917f8d6461faaaf59ecc4eeb3152021-12-02T11:45:53ZThermoresponsive GenisteinNLC-dexamethasone-moxifloxacin multi drug delivery system in lens capsule bag to prevent complications after cataract surgery10.1038/s41598-020-80476-x2045-2322https://doaj.org/article/8fde6917f8d6461faaaf59ecc4eeb3152021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80476-xhttps://doaj.org/toc/2045-2322Abstract Cataract surgery is the most common intraocular procedure. To decrease postsurgical inflammation, prevent infection and reduce the incidence of secondary cataract, we built a temperature-sensitive drug delivery system carrying dexamethasone, moxifloxacin and genistein nanostructured lipid carrier (GenNLC) modified by mPEG-PLA based on F127/F68 as hydrogel. Characterizations and release profiles of the drug delivery system were studied. In vitro functions were detected by CCK-8 test, immunofluorescence, wound-healing assay, real time-PCR and western blotting. The size of GenNLCs was 39.47 ± 0.69 nm in average with surface charges of − 4.32 ± 0.84 mV. The hydrogel gelation temperature and time were 32 °C, 20 s with a viscosity, hardness, adhesiveness and stringiness of 6.135 Pa.s, 54.0 g, 22.0 g, and 3.24 mm, respectively. Transmittance of the gel-release medium was above 90% (93.44 ± 0.33% to 100%) at range of 430 nm to 800 nm. Moxifloxacin released completely within 10 days. Fifty percent of dexamethasone released at a constant rate in the first week, and then released sustainably with a tapering down rate until day 30. Genistein released slowly but persistently with a cumulative release of 63% at day 40. The thermoresponsive hydrogel inhibited the proliferation, migration and epithelial-mesenchymal transition of SRA 01/04 cells, which were confirmed by testing CCK-8, wound-healing assay, western blot, real time-PCR (RT-PCR) and immunofluorescence. These results support this intracameral thermoresponsive in situ multi-drug delivery system with programmed release amounts and release profiles to cut down the need of eye drops for preventing inflammation or infection and to reduce posterior capsular opacification following cataract surgery.Tingyu YanZhongxu MaJingjing LiuNa YinShizhen LeiXinxin ZhangXuedong LiYu ZhangJun KongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tingyu Yan Zhongxu Ma Jingjing Liu Na Yin Shizhen Lei Xinxin Zhang Xuedong Li Yu Zhang Jun Kong Thermoresponsive GenisteinNLC-dexamethasone-moxifloxacin multi drug delivery system in lens capsule bag to prevent complications after cataract surgery
description	Abstract Cataract surgery is the most common intraocular procedure. To decrease postsurgical inflammation, prevent infection and reduce the incidence of secondary cataract, we built a temperature-sensitive drug delivery system carrying dexamethasone, moxifloxacin and genistein nanostructured lipid carrier (GenNLC) modified by mPEG-PLA based on F127/F68 as hydrogel. Characterizations and release profiles of the drug delivery system were studied. In vitro functions were detected by CCK-8 test, immunofluorescence, wound-healing assay, real time-PCR and western blotting. The size of GenNLCs was 39.47 ± 0.69 nm in average with surface charges of − 4.32 ± 0.84 mV. The hydrogel gelation temperature and time were 32 °C, 20 s with a viscosity, hardness, adhesiveness and stringiness of 6.135 Pa.s, 54.0 g, 22.0 g, and 3.24 mm, respectively. Transmittance of the gel-release medium was above 90% (93.44 ± 0.33% to 100%) at range of 430 nm to 800 nm. Moxifloxacin released completely within 10 days. Fifty percent of dexamethasone released at a constant rate in the first week, and then released sustainably with a tapering down rate until day 30. Genistein released slowly but persistently with a cumulative release of 63% at day 40. The thermoresponsive hydrogel inhibited the proliferation, migration and epithelial-mesenchymal transition of SRA 01/04 cells, which were confirmed by testing CCK-8, wound-healing assay, western blot, real time-PCR (RT-PCR) and immunofluorescence. These results support this intracameral thermoresponsive in situ multi-drug delivery system with programmed release amounts and release profiles to cut down the need of eye drops for preventing inflammation or infection and to reduce posterior capsular opacification following cataract surgery.
format	article
author	Tingyu Yan Zhongxu Ma Jingjing Liu Na Yin Shizhen Lei Xinxin Zhang Xuedong Li Yu Zhang Jun Kong
author_facet	Tingyu Yan Zhongxu Ma Jingjing Liu Na Yin Shizhen Lei Xinxin Zhang Xuedong Li Yu Zhang Jun Kong
author_sort	Tingyu Yan
title	Thermoresponsive GenisteinNLC-dexamethasone-moxifloxacin multi drug delivery system in lens capsule bag to prevent complications after cataract surgery
title_short	Thermoresponsive GenisteinNLC-dexamethasone-moxifloxacin multi drug delivery system in lens capsule bag to prevent complications after cataract surgery
title_full	Thermoresponsive GenisteinNLC-dexamethasone-moxifloxacin multi drug delivery system in lens capsule bag to prevent complications after cataract surgery
title_fullStr	Thermoresponsive GenisteinNLC-dexamethasone-moxifloxacin multi drug delivery system in lens capsule bag to prevent complications after cataract surgery
title_full_unstemmed	Thermoresponsive GenisteinNLC-dexamethasone-moxifloxacin multi drug delivery system in lens capsule bag to prevent complications after cataract surgery
title_sort	thermoresponsive genisteinnlc-dexamethasone-moxifloxacin multi drug delivery system in lens capsule bag to prevent complications after cataract surgery
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8fde6917f8d6461faaaf59ecc4eeb315
work_keys_str_mv	AT tingyuyan thermoresponsivegenisteinnlcdexamethasonemoxifloxacinmultidrugdeliverysysteminlenscapsulebagtopreventcomplicationsaftercataractsurgery AT zhongxuma thermoresponsivegenisteinnlcdexamethasonemoxifloxacinmultidrugdeliverysysteminlenscapsulebagtopreventcomplicationsaftercataractsurgery AT jingjingliu thermoresponsivegenisteinnlcdexamethasonemoxifloxacinmultidrugdeliverysysteminlenscapsulebagtopreventcomplicationsaftercataractsurgery AT nayin thermoresponsivegenisteinnlcdexamethasonemoxifloxacinmultidrugdeliverysysteminlenscapsulebagtopreventcomplicationsaftercataractsurgery AT shizhenlei thermoresponsivegenisteinnlcdexamethasonemoxifloxacinmultidrugdeliverysysteminlenscapsulebagtopreventcomplicationsaftercataractsurgery AT xinxinzhang thermoresponsivegenisteinnlcdexamethasonemoxifloxacinmultidrugdeliverysysteminlenscapsulebagtopreventcomplicationsaftercataractsurgery AT xuedongli thermoresponsivegenisteinnlcdexamethasonemoxifloxacinmultidrugdeliverysysteminlenscapsulebagtopreventcomplicationsaftercataractsurgery AT yuzhang thermoresponsivegenisteinnlcdexamethasonemoxifloxacinmultidrugdeliverysysteminlenscapsulebagtopreventcomplicationsaftercataractsurgery AT junkong thermoresponsivegenisteinnlcdexamethasonemoxifloxacinmultidrugdeliverysysteminlenscapsulebagtopreventcomplicationsaftercataractsurgery
_version_	1718395239741259776

Thermoresponsive GenisteinNLC-dexamethasone-moxifloxacin multi drug delivery system in lens capsule bag to prevent complications after cataract surgery

Ejemplares similares