A panel of 8-lncRNA predicts prognosis of breast cancer patients and migration of breast cancer cells.

<h4>Background</h4>Breast cancer (BCa) is the most commonly diagnosed cancer and the leading cause of cancer death among females around the world. Recent studies have indicated that long non-coding RNAs (lncRNAs) can serve as an independent biomarker for diagnosis and prognosis in many t...

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Autores principales:	Lili Zhu, Kang Cui, Lanling Weng, Pu Yu, Yabing Du, Tengfei Zhang, Hong Liu, Bingjie Li, Wang Ma
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8fded7e57dc446f59644555f2a212504
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spelling	oai:doaj.org-article:8fded7e57dc446f59644555f2a2125042021-12-02T20:03:57ZA panel of 8-lncRNA predicts prognosis of breast cancer patients and migration of breast cancer cells.1932-620310.1371/journal.pone.0249174https://doaj.org/article/8fded7e57dc446f59644555f2a2125042021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0249174https://doaj.org/toc/1932-6203<h4>Background</h4>Breast cancer (BCa) is the most commonly diagnosed cancer and the leading cause of cancer death among females around the world. Recent studies have indicated that long non-coding RNAs (lncRNAs) can serve as an independent biomarker for diagnosis and prognosis in many types of cancer, including pancreatic adenocarcinoma, gastric cancer, liver cancer, and lung cancer. Previous studies have shown that many lncRNAs are associated with the occurrence and development of BCa. However, few studies have combined multiple lncRNAs to predict the prognosis of early-stage BCa patients.<h4>Methods</h4>Systematic and comprehensive analysis of data from The Cancer Genome Atlas (TCGA) was conducted to identify lncRNA signatures with prognostic value in BCa. Additionally, the relative expression levels of the 8 lncRNA of several BCa cell lines were detected by quantitative real-time PCR (qPCR) and the results were substituted into a risk score formula. Finally, migration assays were used to verify the result from prognostic analysis according to the risk scores among cell lines with different risk scores.<h4>Results</h4>Our study included 808 BCa patients with complete clinical data. A panel of 8 lncRNAs was identified using Wilcox tests as different between normal and tumor tissue of the BCa patients. This panel was used to analyze the survival of BCa patients. Patients with low risk scores had greater overall survival (OS) than those with high risk scores. Multivariate Cox regression analyses demonstrated that the lncRNA signature was an independent prognostic factor. Gene Set Enrichment Analysis (GSEA) suggested that the lncRNAs might be involved in several molecular signaling pathways implicated in BCa such as the DNA replication pathway, the cell cycle pathway, and the pentose phosphate pathway. Validation experiments in breast cancer cells to test cell migration by using wound-healing assays supported the results of the model.<h4>Conclusion</h4>Our study demonstrated that a panel of 8 lncRNAs has the potential to be used as an independent prognostic biomarker of BCa.Lili ZhuKang CuiLanling WengPu YuYabing DuTengfei ZhangHong LiuBingjie LiWang MaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0249174 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lili Zhu Kang Cui Lanling Weng Pu Yu Yabing Du Tengfei Zhang Hong Liu Bingjie Li Wang Ma A panel of 8-lncRNA predicts prognosis of breast cancer patients and migration of breast cancer cells.
description	<h4>Background</h4>Breast cancer (BCa) is the most commonly diagnosed cancer and the leading cause of cancer death among females around the world. Recent studies have indicated that long non-coding RNAs (lncRNAs) can serve as an independent biomarker for diagnosis and prognosis in many types of cancer, including pancreatic adenocarcinoma, gastric cancer, liver cancer, and lung cancer. Previous studies have shown that many lncRNAs are associated with the occurrence and development of BCa. However, few studies have combined multiple lncRNAs to predict the prognosis of early-stage BCa patients.<h4>Methods</h4>Systematic and comprehensive analysis of data from The Cancer Genome Atlas (TCGA) was conducted to identify lncRNA signatures with prognostic value in BCa. Additionally, the relative expression levels of the 8 lncRNA of several BCa cell lines were detected by quantitative real-time PCR (qPCR) and the results were substituted into a risk score formula. Finally, migration assays were used to verify the result from prognostic analysis according to the risk scores among cell lines with different risk scores.<h4>Results</h4>Our study included 808 BCa patients with complete clinical data. A panel of 8 lncRNAs was identified using Wilcox tests as different between normal and tumor tissue of the BCa patients. This panel was used to analyze the survival of BCa patients. Patients with low risk scores had greater overall survival (OS) than those with high risk scores. Multivariate Cox regression analyses demonstrated that the lncRNA signature was an independent prognostic factor. Gene Set Enrichment Analysis (GSEA) suggested that the lncRNAs might be involved in several molecular signaling pathways implicated in BCa such as the DNA replication pathway, the cell cycle pathway, and the pentose phosphate pathway. Validation experiments in breast cancer cells to test cell migration by using wound-healing assays supported the results of the model.<h4>Conclusion</h4>Our study demonstrated that a panel of 8 lncRNAs has the potential to be used as an independent prognostic biomarker of BCa.
format	article
author	Lili Zhu Kang Cui Lanling Weng Pu Yu Yabing Du Tengfei Zhang Hong Liu Bingjie Li Wang Ma
author_facet	Lili Zhu Kang Cui Lanling Weng Pu Yu Yabing Du Tengfei Zhang Hong Liu Bingjie Li Wang Ma
author_sort	Lili Zhu
title	A panel of 8-lncRNA predicts prognosis of breast cancer patients and migration of breast cancer cells.
title_short	A panel of 8-lncRNA predicts prognosis of breast cancer patients and migration of breast cancer cells.
title_full	A panel of 8-lncRNA predicts prognosis of breast cancer patients and migration of breast cancer cells.
title_fullStr	A panel of 8-lncRNA predicts prognosis of breast cancer patients and migration of breast cancer cells.
title_full_unstemmed	A panel of 8-lncRNA predicts prognosis of breast cancer patients and migration of breast cancer cells.
title_sort	panel of 8-lncrna predicts prognosis of breast cancer patients and migration of breast cancer cells.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/8fded7e57dc446f59644555f2a212504
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A panel of 8-lncRNA predicts prognosis of breast cancer patients and migration of breast cancer cells.

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