MacroGreen, a simple tool for detection of ADP-ribosylated proteins

García Saura et al. report a new tool, MacroGreen, to detect ADP-ribosylation by GFP fluorescence in a microplate reader, or in cells by microscopy. With superior affinity and reduced ADP-ribosyl hydrolase activity, MacroGreen is an easy to produce and suitable tool for rapid detection of ADP-ribosy...

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Autores principales:	Antonio Ginés García-Saura, Laura K. Herzog, Nico P. Dantuma, Herwig Schüler
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/8fe7a8dba01b4c11be93a83d1e113985
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spelling	oai:doaj.org-article:8fe7a8dba01b4c11be93a83d1e1139852021-12-02T16:24:51ZMacroGreen, a simple tool for detection of ADP-ribosylated proteins10.1038/s42003-021-02439-w2399-3642https://doaj.org/article/8fe7a8dba01b4c11be93a83d1e1139852021-07-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02439-whttps://doaj.org/toc/2399-3642García Saura et al. report a new tool, MacroGreen, to detect ADP-ribosylation by GFP fluorescence in a microplate reader, or in cells by microscopy. With superior affinity and reduced ADP-ribosyl hydrolase activity, MacroGreen is an easy to produce and suitable tool for rapid detection of ADP-ribosylated proteins in vitro without a need for specialist reagents and time-consuming methods.Antonio Ginés García-SauraLaura K. HerzogNico P. DantumaHerwig SchülerNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Antonio Ginés García-Saura Laura K. Herzog Nico P. Dantuma Herwig Schüler MacroGreen, a simple tool for detection of ADP-ribosylated proteins
description	García Saura et al. report a new tool, MacroGreen, to detect ADP-ribosylation by GFP fluorescence in a microplate reader, or in cells by microscopy. With superior affinity and reduced ADP-ribosyl hydrolase activity, MacroGreen is an easy to produce and suitable tool for rapid detection of ADP-ribosylated proteins in vitro without a need for specialist reagents and time-consuming methods.
format	article
author	Antonio Ginés García-Saura Laura K. Herzog Nico P. Dantuma Herwig Schüler
author_facet	Antonio Ginés García-Saura Laura K. Herzog Nico P. Dantuma Herwig Schüler
author_sort	Antonio Ginés García-Saura
title	MacroGreen, a simple tool for detection of ADP-ribosylated proteins
title_short	MacroGreen, a simple tool for detection of ADP-ribosylated proteins
title_full	MacroGreen, a simple tool for detection of ADP-ribosylated proteins
title_fullStr	MacroGreen, a simple tool for detection of ADP-ribosylated proteins
title_full_unstemmed	MacroGreen, a simple tool for detection of ADP-ribosylated proteins
title_sort	macrogreen, a simple tool for detection of adp-ribosylated proteins
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8fe7a8dba01b4c11be93a83d1e113985
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MacroGreen, a simple tool for detection of ADP-ribosylated proteins

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