Investigation of Candida parapsilosis virulence regulatory factors during host-pathogen interaction

Abstract Invasive candidiasis is among the most life-threatening infections in patients in intensive care units. Although Candida albicans is the leading cause of candidaemia, the incidence of Candida parapsilosis infections is also rising, particularly among the neonates. Due to differences in thei...

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Autores principales:	Renáta Tóth, Vitor Cabral, Ernst Thuer, Flóra Bohner, Tibor Németh, Csaba Papp, Leonardo Nimrichter, Gergő Molnár, Csaba Vágvölgyi, Toni Gabaldón, Joshua D. Nosanchuk, Attila Gácser
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8fe993848e92420ea58d8136486cacf5
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spelling	oai:doaj.org-article:8fe993848e92420ea58d8136486cacf52021-12-02T15:08:45ZInvestigation of Candida parapsilosis virulence regulatory factors during host-pathogen interaction10.1038/s41598-018-19453-42045-2322https://doaj.org/article/8fe993848e92420ea58d8136486cacf52018-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-19453-4https://doaj.org/toc/2045-2322Abstract Invasive candidiasis is among the most life-threatening infections in patients in intensive care units. Although Candida albicans is the leading cause of candidaemia, the incidence of Candida parapsilosis infections is also rising, particularly among the neonates. Due to differences in their biology, these species employ different antifungal resistance and virulence mechanisms and also induce dissimilar immune responses. Previously, it has been suggested that core virulence effecting transcription regulators could be attractive ligands for future antifungal drugs. Although the virulence regulatory mechanisms of C. albicans are well studied, less is known about similar mechanisms in C. parapsilosis. In order to search for potential targets for future antifungal drugs against this species, we analyzed the fungal transcriptome during host-pathogen interaction using an in vitro infection model. Selected genes with high expression levels were further examined through their respective null mutant strains, under conditions that mimic the host environment or influence pathogenicity. As a result, we identified several mutants with relevant pathogenicity affecting phenotypes. During the study we highlight three potentially tractable signaling regulators that influence C. parapsilosis pathogenicity in distinct mechanisms. During infection, CPAR2_100540 is responsible for nutrient acquisition, CPAR2_200390 for cell wall assembly and morphology switching and CPAR2_303700 for fungal viability.Renáta TóthVitor CabralErnst ThuerFlóra BohnerTibor NémethCsaba PappLeonardo NimrichterGergő MolnárCsaba VágvölgyiToni GabaldónJoshua D. NosanchukAttila GácserNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-14 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Renáta Tóth Vitor Cabral Ernst Thuer Flóra Bohner Tibor Németh Csaba Papp Leonardo Nimrichter Gergő Molnár Csaba Vágvölgyi Toni Gabaldón Joshua D. Nosanchuk Attila Gácser Investigation of Candida parapsilosis virulence regulatory factors during host-pathogen interaction
description	Abstract Invasive candidiasis is among the most life-threatening infections in patients in intensive care units. Although Candida albicans is the leading cause of candidaemia, the incidence of Candida parapsilosis infections is also rising, particularly among the neonates. Due to differences in their biology, these species employ different antifungal resistance and virulence mechanisms and also induce dissimilar immune responses. Previously, it has been suggested that core virulence effecting transcription regulators could be attractive ligands for future antifungal drugs. Although the virulence regulatory mechanisms of C. albicans are well studied, less is known about similar mechanisms in C. parapsilosis. In order to search for potential targets for future antifungal drugs against this species, we analyzed the fungal transcriptome during host-pathogen interaction using an in vitro infection model. Selected genes with high expression levels were further examined through their respective null mutant strains, under conditions that mimic the host environment or influence pathogenicity. As a result, we identified several mutants with relevant pathogenicity affecting phenotypes. During the study we highlight three potentially tractable signaling regulators that influence C. parapsilosis pathogenicity in distinct mechanisms. During infection, CPAR2_100540 is responsible for nutrient acquisition, CPAR2_200390 for cell wall assembly and morphology switching and CPAR2_303700 for fungal viability.
format	article
author	Renáta Tóth Vitor Cabral Ernst Thuer Flóra Bohner Tibor Németh Csaba Papp Leonardo Nimrichter Gergő Molnár Csaba Vágvölgyi Toni Gabaldón Joshua D. Nosanchuk Attila Gácser
author_facet	Renáta Tóth Vitor Cabral Ernst Thuer Flóra Bohner Tibor Németh Csaba Papp Leonardo Nimrichter Gergő Molnár Csaba Vágvölgyi Toni Gabaldón Joshua D. Nosanchuk Attila Gácser
author_sort	Renáta Tóth
title	Investigation of Candida parapsilosis virulence regulatory factors during host-pathogen interaction
title_short	Investigation of Candida parapsilosis virulence regulatory factors during host-pathogen interaction
title_full	Investigation of Candida parapsilosis virulence regulatory factors during host-pathogen interaction
title_fullStr	Investigation of Candida parapsilosis virulence regulatory factors during host-pathogen interaction
title_full_unstemmed	Investigation of Candida parapsilosis virulence regulatory factors during host-pathogen interaction
title_sort	investigation of candida parapsilosis virulence regulatory factors during host-pathogen interaction
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/8fe993848e92420ea58d8136486cacf5
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Investigation of Candida parapsilosis virulence regulatory factors during host-pathogen interaction

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