A transcriptome atlas of the mouse iris at single-cell resolution defines cell types and the genomic response to pupil dilation

The iris controls the level of retinal illumination by controlling pupil diameter. It is a site of diverse ophthalmologic diseases and it is a potential source of cells for ocular auto-transplantation. The present study provides foundational data on the mouse iris based on single nucleus RNA sequenc...

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Autores principales:	Jie Wang, Amir Rattner, Jeremy Nathans
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	pupils ocular structure smooth muscle single-cell sequencing Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/8fea2d0934074d4982e113780fd77067
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spelling	oai:doaj.org-article:8fea2d0934074d4982e113780fd770672021-11-16T12:30:36ZA transcriptome atlas of the mouse iris at single-cell resolution defines cell types and the genomic response to pupil dilation10.7554/eLife.734772050-084Xe73477https://doaj.org/article/8fea2d0934074d4982e113780fd770672021-11-01T00:00:00Zhttps://elifesciences.org/articles/73477https://doaj.org/toc/2050-084XThe iris controls the level of retinal illumination by controlling pupil diameter. It is a site of diverse ophthalmologic diseases and it is a potential source of cells for ocular auto-transplantation. The present study provides foundational data on the mouse iris based on single nucleus RNA sequencing. More specifically, this work has (1) defined all of the major cell types in the mouse iris and ciliary body, (2) led to the discovery of two types of iris stromal cells and two types of iris sphincter cells, (3) revealed the differences in cell type-specific transcriptomes in the resting vs. dilated states, and (4) identified and validated antibody and in situ hybridization probes that can be used to visualize the major iris cell types. By immunostaining for specific iris cell types, we have observed and quantified distortions in nuclear morphology associated with iris dilation and clarified the neural crest contribution to the iris by showing that Wnt1-Cre-expressing progenitors contribute to nearly all iris cell types, whereas Sox10-Cre-expressing progenitors contribute only to stromal cells. This work should be useful as a point of reference for investigations of iris development, disease, and pharmacology, for the isolation and propagation of defined iris cell types, and for iris cell engineering and transplantation.Jie WangAmir RattnerJeremy NathanseLife Sciences Publications Ltdarticlepupilsocular structuresmooth musclesingle-cell sequencingMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	pupils ocular structure smooth muscle single-cell sequencing Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	pupils ocular structure smooth muscle single-cell sequencing Medicine R Science Q Biology (General) QH301-705.5 Jie Wang Amir Rattner Jeremy Nathans A transcriptome atlas of the mouse iris at single-cell resolution defines cell types and the genomic response to pupil dilation
description	The iris controls the level of retinal illumination by controlling pupil diameter. It is a site of diverse ophthalmologic diseases and it is a potential source of cells for ocular auto-transplantation. The present study provides foundational data on the mouse iris based on single nucleus RNA sequencing. More specifically, this work has (1) defined all of the major cell types in the mouse iris and ciliary body, (2) led to the discovery of two types of iris stromal cells and two types of iris sphincter cells, (3) revealed the differences in cell type-specific transcriptomes in the resting vs. dilated states, and (4) identified and validated antibody and in situ hybridization probes that can be used to visualize the major iris cell types. By immunostaining for specific iris cell types, we have observed and quantified distortions in nuclear morphology associated with iris dilation and clarified the neural crest contribution to the iris by showing that Wnt1-Cre-expressing progenitors contribute to nearly all iris cell types, whereas Sox10-Cre-expressing progenitors contribute only to stromal cells. This work should be useful as a point of reference for investigations of iris development, disease, and pharmacology, for the isolation and propagation of defined iris cell types, and for iris cell engineering and transplantation.
format	article
author	Jie Wang Amir Rattner Jeremy Nathans
author_facet	Jie Wang Amir Rattner Jeremy Nathans
author_sort	Jie Wang
title	A transcriptome atlas of the mouse iris at single-cell resolution defines cell types and the genomic response to pupil dilation
title_short	A transcriptome atlas of the mouse iris at single-cell resolution defines cell types and the genomic response to pupil dilation
title_full	A transcriptome atlas of the mouse iris at single-cell resolution defines cell types and the genomic response to pupil dilation
title_fullStr	A transcriptome atlas of the mouse iris at single-cell resolution defines cell types and the genomic response to pupil dilation
title_full_unstemmed	A transcriptome atlas of the mouse iris at single-cell resolution defines cell types and the genomic response to pupil dilation
title_sort	transcriptome atlas of the mouse iris at single-cell resolution defines cell types and the genomic response to pupil dilation
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/8fea2d0934074d4982e113780fd77067
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A transcriptome atlas of the mouse iris at single-cell resolution defines cell types and the genomic response to pupil dilation

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