Interactions of anaerobic bacteria with dental stem cells: an in vitro study.

<h4>Background</h4>In patients with periodontitis, it is highly likely that local (progenitor) cells encounter pathogenic bacteria. The purpose of this in vitro study was to elucidate how human dental follicle stem cells (hDFSC) react towards a direct challenge with anaerobic periodontal...

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Autores principales:	Anne Biedermann, Katja Kriebel, Bernd Kreikemeyer, Hermann Lang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8febc296985e4ef2b366573ce80aa0b9
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spelling	oai:doaj.org-article:8febc296985e4ef2b366573ce80aa0b92021-11-25T05:54:41ZInteractions of anaerobic bacteria with dental stem cells: an in vitro study.1932-620310.1371/journal.pone.0110616https://doaj.org/article/8febc296985e4ef2b366573ce80aa0b92014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0110616https://doaj.org/toc/1932-6203<h4>Background</h4>In patients with periodontitis, it is highly likely that local (progenitor) cells encounter pathogenic bacteria. The purpose of this in vitro study was to elucidate how human dental follicle stem cells (hDFSC) react towards a direct challenge with anaerobic periodontal pathogens under their natural oxygen-free atmosphere. HDFSC were compared to human bone marrow mesenchymal stem cells (hBMSC) and differentiated primary human gingival fibroblasts (hGiF), as well as permanent gingival carcinoma cells (Ca9-22).<h4>Methodology/principal findings</h4>The different cell types were investigated in a co-culture system with Porphyromonas gingivalis (P. gingivalis) and Fusobacterium nucleatum (F. nucleatum). The viability of the cells and pathogens under anaerobic conditions, as well as interactions in terms of adherence and internalization, were examined. Additionally, the release of pro-inflammatory interleukin-8 (IL-8) and anti-inflammatory interleukin-10 (IL-10) was quantified via enzyme-linked immunosorbent assay. The bacteria adhered less efficiently to hDFSC compared to Ca9-22 (P. gingivalis: 0.18% adherence to hDFSC; 3.1% adherence to Ca9-22). Similar results were observed for host cell internalization (F. nucleatum: 0.002% internalization into hDFSC; 0.09% internalization into Ca9-22). Statistically significantly less IL-8 was secreted from hDFSC after stimulation with F. nucleatum and P. gingivalis in comparison with hGiF (F. nucleatum: 2080.0 pg/ml--hGiF; 19.7 pg/ml--hDFSC). The IL-10 response of the differentiated cells was found to be low in relation to their pro-inflammatory IL-8 response.<h4>Conclusions/significance</h4>The results indicate that dental stem cells are less prone to interactions with pathogenic bacteria than differentiated cells in an anaerobic environment. Moreover, during bacterial challenge, the stem cell immune response seems to be more towards an anti-inflammatory reaction. For a potential future therapeutic use of hDFSC, these findings support the idea of a save application.Anne BiedermannKatja KriebelBernd KreikemeyerHermann LangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 11, p e110616 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anne Biedermann Katja Kriebel Bernd Kreikemeyer Hermann Lang Interactions of anaerobic bacteria with dental stem cells: an in vitro study.
description	<h4>Background</h4>In patients with periodontitis, it is highly likely that local (progenitor) cells encounter pathogenic bacteria. The purpose of this in vitro study was to elucidate how human dental follicle stem cells (hDFSC) react towards a direct challenge with anaerobic periodontal pathogens under their natural oxygen-free atmosphere. HDFSC were compared to human bone marrow mesenchymal stem cells (hBMSC) and differentiated primary human gingival fibroblasts (hGiF), as well as permanent gingival carcinoma cells (Ca9-22).<h4>Methodology/principal findings</h4>The different cell types were investigated in a co-culture system with Porphyromonas gingivalis (P. gingivalis) and Fusobacterium nucleatum (F. nucleatum). The viability of the cells and pathogens under anaerobic conditions, as well as interactions in terms of adherence and internalization, were examined. Additionally, the release of pro-inflammatory interleukin-8 (IL-8) and anti-inflammatory interleukin-10 (IL-10) was quantified via enzyme-linked immunosorbent assay. The bacteria adhered less efficiently to hDFSC compared to Ca9-22 (P. gingivalis: 0.18% adherence to hDFSC; 3.1% adherence to Ca9-22). Similar results were observed for host cell internalization (F. nucleatum: 0.002% internalization into hDFSC; 0.09% internalization into Ca9-22). Statistically significantly less IL-8 was secreted from hDFSC after stimulation with F. nucleatum and P. gingivalis in comparison with hGiF (F. nucleatum: 2080.0 pg/ml--hGiF; 19.7 pg/ml--hDFSC). The IL-10 response of the differentiated cells was found to be low in relation to their pro-inflammatory IL-8 response.<h4>Conclusions/significance</h4>The results indicate that dental stem cells are less prone to interactions with pathogenic bacteria than differentiated cells in an anaerobic environment. Moreover, during bacterial challenge, the stem cell immune response seems to be more towards an anti-inflammatory reaction. For a potential future therapeutic use of hDFSC, these findings support the idea of a save application.
format	article
author	Anne Biedermann Katja Kriebel Bernd Kreikemeyer Hermann Lang
author_facet	Anne Biedermann Katja Kriebel Bernd Kreikemeyer Hermann Lang
author_sort	Anne Biedermann
title	Interactions of anaerobic bacteria with dental stem cells: an in vitro study.
title_short	Interactions of anaerobic bacteria with dental stem cells: an in vitro study.
title_full	Interactions of anaerobic bacteria with dental stem cells: an in vitro study.
title_fullStr	Interactions of anaerobic bacteria with dental stem cells: an in vitro study.
title_full_unstemmed	Interactions of anaerobic bacteria with dental stem cells: an in vitro study.
title_sort	interactions of anaerobic bacteria with dental stem cells: an in vitro study.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/8febc296985e4ef2b366573ce80aa0b9
work_keys_str_mv	AT annebiedermann interactionsofanaerobicbacteriawithdentalstemcellsaninvitrostudy AT katjakriebel interactionsofanaerobicbacteriawithdentalstemcellsaninvitrostudy AT berndkreikemeyer interactionsofanaerobicbacteriawithdentalstemcellsaninvitrostudy AT hermannlang interactionsofanaerobicbacteriawithdentalstemcellsaninvitrostudy
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Interactions of anaerobic bacteria with dental stem cells: an in vitro study.

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