Preliminary biocompatibility investigation of magnetic albumin nanosphere designed as a potential versatile drug delivery system

Preliminary biocompatibility investigation of magnetic albumin nanosphere designed as a potential versatile drug delivery system

Luciana Estevanato1, Débora Cintra1, Nayara Baldini1, Flávia Portilho1, Luzirlane Barbosa1, Olímpia Martins2, Bruno Lacava3, Ana Luisa Miranda-Vilela1, Antônio Cláudio Tedesco2, Sônia Báo1, Paulo C...

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Autores principales: Estevanato L, Cintra D, Baldini N, Portilho F, Barbosa L, Martins O, Lacava B, Miranda-Vilela AL, Tedesco AC, Báo S, Morais PC, Lacava ZGM
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2011
Materias:
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/8ff01c4d4ad440528fdd04a93f723579
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spelling oai:doaj.org-article:8ff01c4d4ad440528fdd04a93f7235792021-12-02T01:32:22ZPreliminary biocompatibility investigation of magnetic albumin nanosphere designed as a potential versatile drug delivery system1176-91141178-2013https://doaj.org/article/8ff01c4d4ad440528fdd04a93f7235792011-08-01T00:00:00Zhttp://www.dovepress.com/preliminary-biocompatibility-investigation-of-magnetic-albumin-nanosph-a8098https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Luciana Estevanato1, Débora Cintra1, Nayara Baldini1, Flávia Portilho1, Luzirlane Barbosa1, Olímpia Martins2, Bruno Lacava3, Ana Luisa Miranda-Vilela1, Antônio Cláudio Tedesco2, Sônia Báo1, Paulo C Morais4, Zulmira GM Lacava11Instituto de Ciências Biológicas, Universidade de Brasília, 2Departamento de Química, Laboratório de Fotobiologia e Fotomedicina, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, 3Instituto de Química, Universidade de Brasília, Brasília, 4Instituto de Física, Universidade de Brasília, Brasília, BrazilBackground: The magnetic albumin nanosphere (MAN), encapsulating maghemite nanoparticles, was designed as a magnetic drug delivery system (MDDS) able to perform a variety of biomedical applications. It is noteworthy that MAN was efficient in treating Ehrlich's tumors by the magnetohyperthermia procedure.Methods and materials: In this study, several nanotoxicity tests were systematically carried out in mice from 30 minutes until 30 days after MAN injection to investigate their biocompatibility status. Cytometry analysis, viability tests, micronucleus assay, and histological analysis were performed.Results: Cytometry analysis and viability tests revealed MAN promotes only slight and temporary alterations in the frequency of both leukocyte populations and viable peritoneal cells, respectively. Micronucleus assay showed absolutely no genotoxicity or cytotoxicity effects and histological analysis showed no alterations or even nanoparticle clusters in several investigated organs but, interestingly, revealed the presence of MAN clusters in the central nervous system (CNS).Conclusion: The results showed that MAN has desirable in vivo biocompatibility, presenting potential for use as a MDDS, especially in CNS disease therapy.Keywords: nanotoxicity, nanoparticle, genotoxicity, cytotoxicity, brainEstevanato LCintra DBaldini NPortilho FBarbosa LMartins OLacava BMiranda-Vilela ALTedesco ACBáo SMorais PCLacava ZGMDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 1709-1717 (2011)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Estevanato L
Cintra D
Baldini N
Portilho F
Barbosa L
Martins O
Lacava B
Miranda-Vilela AL
Tedesco AC
Báo S
Morais PC
Lacava ZGM
Preliminary biocompatibility investigation of magnetic albumin nanosphere designed as a potential versatile drug delivery system
description Luciana Estevanato1, Débora Cintra1, Nayara Baldini1, Flávia Portilho1, Luzirlane Barbosa1, Olímpia Martins2, Bruno Lacava3, Ana Luisa Miranda-Vilela1, Antônio Cláudio Tedesco2, Sônia Báo1, Paulo C Morais4, Zulmira GM Lacava11Instituto de Ciências Biológicas, Universidade de Brasília, 2Departamento de Química, Laboratório de Fotobiologia e Fotomedicina, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, 3Instituto de Química, Universidade de Brasília, Brasília, 4Instituto de Física, Universidade de Brasília, Brasília, BrazilBackground: The magnetic albumin nanosphere (MAN), encapsulating maghemite nanoparticles, was designed as a magnetic drug delivery system (MDDS) able to perform a variety of biomedical applications. It is noteworthy that MAN was efficient in treating Ehrlich's tumors by the magnetohyperthermia procedure.Methods and materials: In this study, several nanotoxicity tests were systematically carried out in mice from 30 minutes until 30 days after MAN injection to investigate their biocompatibility status. Cytometry analysis, viability tests, micronucleus assay, and histological analysis were performed.Results: Cytometry analysis and viability tests revealed MAN promotes only slight and temporary alterations in the frequency of both leukocyte populations and viable peritoneal cells, respectively. Micronucleus assay showed absolutely no genotoxicity or cytotoxicity effects and histological analysis showed no alterations or even nanoparticle clusters in several investigated organs but, interestingly, revealed the presence of MAN clusters in the central nervous system (CNS).Conclusion: The results showed that MAN has desirable in vivo biocompatibility, presenting potential for use as a MDDS, especially in CNS disease therapy.Keywords: nanotoxicity, nanoparticle, genotoxicity, cytotoxicity, brain
format article
author Estevanato L
Cintra D
Baldini N
Portilho F
Barbosa L
Martins O
Lacava B
Miranda-Vilela AL
Tedesco AC
Báo S
Morais PC
Lacava ZGM
author_facet Estevanato L
Cintra D
Baldini N
Portilho F
Barbosa L
Martins O
Lacava B
Miranda-Vilela AL
Tedesco AC
Báo S
Morais PC
Lacava ZGM
author_sort Estevanato L
title Preliminary biocompatibility investigation of magnetic albumin nanosphere designed as a potential versatile drug delivery system
title_short Preliminary biocompatibility investigation of magnetic albumin nanosphere designed as a potential versatile drug delivery system
title_full Preliminary biocompatibility investigation of magnetic albumin nanosphere designed as a potential versatile drug delivery system
title_fullStr Preliminary biocompatibility investigation of magnetic albumin nanosphere designed as a potential versatile drug delivery system
title_full_unstemmed Preliminary biocompatibility investigation of magnetic albumin nanosphere designed as a potential versatile drug delivery system
title_sort preliminary biocompatibility investigation of magnetic albumin nanosphere designed as a potential versatile drug delivery system
publisher Dove Medical Press
publishDate 2011
url https://doaj.org/article/8ff01c4d4ad440528fdd04a93f723579
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