ROS-induced dramatic lipid changes in Arabidopsis

Objectives: The beneficial role of ROS was probably in promoting intercellular communication by modifying membrane constituents [Liang D. A salutary role of reactive oxygen species in intercellular tunnel-mediated communication. Front Cell Dev Biol. 2018;6:2]. We investigated how the membrane lipids...

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Autores principales:	Tianlin Jin, Xue Wang, Zhuying Deng, Xiaofang Liu, Dacheng Liang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	membrane lipid arabidopsis h2o2 ros ros inhibitor Pathology RB1-214 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/8ff9e8f98d924cf3b990bb45d58aebc9
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spelling	oai:doaj.org-article:8ff9e8f98d924cf3b990bb45d58aebc92021-11-11T14:23:41ZROS-induced dramatic lipid changes in Arabidopsis1351-00021743-292810.1080/13510002.2021.2002001https://doaj.org/article/8ff9e8f98d924cf3b990bb45d58aebc92021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/13510002.2021.2002001https://doaj.org/toc/1351-0002https://doaj.org/toc/1743-2928Objectives: The beneficial role of ROS was probably in promoting intercellular communication by modifying membrane constituents [Liang D. A salutary role of reactive oxygen species in intercellular tunnel-mediated communication. Front Cell Dev Biol. 2018;6:2]. We investigated how the membrane lipids were responding to ROS and ROS inhibitors. Methods: To examine how ROS affected the lipid profiles, we used thin-layer chromatography to characterize lipid profiles in Arabidopsis plants. Then, the confocal microscopy imaging was used to confirm the change of membrane lipid in a plasma membrane marker line exposed to ROS and ROS inhibitors. Results: We found the relative contents of most lipids in H2O2-treated Arabidopsis plants were increased in roots, rather than in shoots. The increased fluorescent signal of membrane marker induced by H2O2 was mainly enriched in the conductive parts of roots. Several ROS inhibitors also strongly affected the lipid profiles. Among them, diethyldithiocarbamate (DDC) can progressively change the lipid profiles with treatment going on. Membrane marker signal was mainly accumulated in the root tips and epidermal cells after treatment by DDC. Discussion: H2O2 may enhance intercellular communication by inducing different lipid species in the conductive parts of roots. The lipid profiles were widely responding to various ROS reagents and might play a role in intercellular signaling.Tianlin JinXue WangZhuying DengXiaofang LiuDacheng LiangTaylor & Francis Grouparticlemembranelipidarabidopsish2o2rosros inhibitorPathologyRB1-214Biology (General)QH301-705.5ENRedox Report, Vol 26, Iss 1, Pp 190-196 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	membrane lipid arabidopsis h2o2 ros ros inhibitor Pathology RB1-214 Biology (General) QH301-705.5
spellingShingle	membrane lipid arabidopsis h2o2 ros ros inhibitor Pathology RB1-214 Biology (General) QH301-705.5 Tianlin Jin Xue Wang Zhuying Deng Xiaofang Liu Dacheng Liang ROS-induced dramatic lipid changes in Arabidopsis
description	Objectives: The beneficial role of ROS was probably in promoting intercellular communication by modifying membrane constituents [Liang D. A salutary role of reactive oxygen species in intercellular tunnel-mediated communication. Front Cell Dev Biol. 2018;6:2]. We investigated how the membrane lipids were responding to ROS and ROS inhibitors. Methods: To examine how ROS affected the lipid profiles, we used thin-layer chromatography to characterize lipid profiles in Arabidopsis plants. Then, the confocal microscopy imaging was used to confirm the change of membrane lipid in a plasma membrane marker line exposed to ROS and ROS inhibitors. Results: We found the relative contents of most lipids in H2O2-treated Arabidopsis plants were increased in roots, rather than in shoots. The increased fluorescent signal of membrane marker induced by H2O2 was mainly enriched in the conductive parts of roots. Several ROS inhibitors also strongly affected the lipid profiles. Among them, diethyldithiocarbamate (DDC) can progressively change the lipid profiles with treatment going on. Membrane marker signal was mainly accumulated in the root tips and epidermal cells after treatment by DDC. Discussion: H2O2 may enhance intercellular communication by inducing different lipid species in the conductive parts of roots. The lipid profiles were widely responding to various ROS reagents and might play a role in intercellular signaling.
format	article
author	Tianlin Jin Xue Wang Zhuying Deng Xiaofang Liu Dacheng Liang
author_facet	Tianlin Jin Xue Wang Zhuying Deng Xiaofang Liu Dacheng Liang
author_sort	Tianlin Jin
title	ROS-induced dramatic lipid changes in Arabidopsis
title_short	ROS-induced dramatic lipid changes in Arabidopsis
title_full	ROS-induced dramatic lipid changes in Arabidopsis
title_fullStr	ROS-induced dramatic lipid changes in Arabidopsis
title_full_unstemmed	ROS-induced dramatic lipid changes in Arabidopsis
title_sort	ros-induced dramatic lipid changes in arabidopsis
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/8ff9e8f98d924cf3b990bb45d58aebc9
work_keys_str_mv	AT tianlinjin rosinduceddramaticlipidchangesinarabidopsis AT xuewang rosinduceddramaticlipidchangesinarabidopsis AT zhuyingdeng rosinduceddramaticlipidchangesinarabidopsis AT xiaofangliu rosinduceddramaticlipidchangesinarabidopsis AT dachengliang rosinduceddramaticlipidchangesinarabidopsis
_version_	1718438960263331840

ROS-induced dramatic lipid changes in Arabidopsis

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