Effects of high molecular weight poly-γ-glutamic acid on PIGS with porcine preproductive and respiratory syndrome virus (PRRSV) infection

Bacillus subtilis sups. chungkookjang produces a higher molecular mass poly-γ-glutamic acid (γ-PGA). Recently, previous studies have demonstrated immune stimulation and an antitumor effect of the high molecular mass γ-PGA using various mouse models although these effects have not been shown in other...

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Autores principales:	Seo Byoung-Joo, Lee Jee-Hoon, Kang Ick-Jae, Shabir Nadeem, Khatun Amina, Yang Myeon-Sik, Park Chul, Kim Bumseok, Kim Won-Il
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2017
Materias:	antiviral activity immune stimulation neutralizing antibody poly-γ-glutamic acid prrsv Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/8ffc3d3af43a43ffaee573ff5cbf74ab
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spelling	oai:doaj.org-article:8ffc3d3af43a43ffaee573ff5cbf74ab2021-11-17T21:27:51ZEffects of high molecular weight poly-γ-glutamic acid on PIGS with porcine preproductive and respiratory syndrome virus (PRRSV) infection1820-744810.1515/acve-2017-0014https://doaj.org/article/8ffc3d3af43a43ffaee573ff5cbf74ab2017-06-01T00:00:00Zhttps://doi.org/10.1515/acve-2017-0014https://doaj.org/toc/1820-7448Bacillus subtilis sups. chungkookjang produces a higher molecular mass poly-γ-glutamic acid (γ-PGA). Recently, previous studies have demonstrated immune stimulation and an antitumor effect of the high molecular mass γ-PGA using various mouse models although these effects have not been shown in other species of animals. Therefore, the current study was conducted to determine the effect of γ-PGA in pigs with and without PRRSV infection. PRRS-negative pigs were intramuscularly injected with 1, 3, or 5 ml of 20 mg/mll γ-PGA, and one group of pigs served as a non-treatment (NT) group. All groups treated with γ-PGA had significantly higher weight gains, and pigs treated with 5 ml of γ-PGA exhibited higher tumor necrosis factor (TNF)-α, interferon (IFN)-α and IFN-β expression levels compared with the NT group. According to the preliminary results, an animal challenge study was conducted with a highly virulent PRRSV strain, MN184, along with γ-PGA treatment at different time points. Pigs treated with γ-PGA had lower levels of viral loads in the sera and in lungs and gained significantly more weight (p<0.05) compared with the NT group after being challenged with MN184. Moreover, γ-PGA-treatment groups had higher levels of neutralizing antibodies and cytokines related to proinflammatory, humoral and cell-mediated responses than the control group after the PRRSV challenge. Therefore, it was concluded that γ-PGA induces higher levels of immune responses and increases resistance to PRRSV infection in pigs.Seo Byoung-JooLee Jee-HoonKang Ick-JaeShabir NadeemKhatun AminaYang Myeon-SikPark ChulKim BumseokKim Won-IlSciendoarticleantiviral activityimmune stimulationneutralizing antibodypoly-γ-glutamic acidprrsvVeterinary medicineSF600-1100ENActa Veterinaria, Vol 67, Iss 2, Pp 153-167 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	antiviral activity immune stimulation neutralizing antibody poly-γ-glutamic acid prrsv Veterinary medicine SF600-1100
spellingShingle	antiviral activity immune stimulation neutralizing antibody poly-γ-glutamic acid prrsv Veterinary medicine SF600-1100 Seo Byoung-Joo Lee Jee-Hoon Kang Ick-Jae Shabir Nadeem Khatun Amina Yang Myeon-Sik Park Chul Kim Bumseok Kim Won-Il Effects of high molecular weight poly-γ-glutamic acid on PIGS with porcine preproductive and respiratory syndrome virus (PRRSV) infection
description	Bacillus subtilis sups. chungkookjang produces a higher molecular mass poly-γ-glutamic acid (γ-PGA). Recently, previous studies have demonstrated immune stimulation and an antitumor effect of the high molecular mass γ-PGA using various mouse models although these effects have not been shown in other species of animals. Therefore, the current study was conducted to determine the effect of γ-PGA in pigs with and without PRRSV infection. PRRS-negative pigs were intramuscularly injected with 1, 3, or 5 ml of 20 mg/mll γ-PGA, and one group of pigs served as a non-treatment (NT) group. All groups treated with γ-PGA had significantly higher weight gains, and pigs treated with 5 ml of γ-PGA exhibited higher tumor necrosis factor (TNF)-α, interferon (IFN)-α and IFN-β expression levels compared with the NT group. According to the preliminary results, an animal challenge study was conducted with a highly virulent PRRSV strain, MN184, along with γ-PGA treatment at different time points. Pigs treated with γ-PGA had lower levels of viral loads in the sera and in lungs and gained significantly more weight (p<0.05) compared with the NT group after being challenged with MN184. Moreover, γ-PGA-treatment groups had higher levels of neutralizing antibodies and cytokines related to proinflammatory, humoral and cell-mediated responses than the control group after the PRRSV challenge. Therefore, it was concluded that γ-PGA induces higher levels of immune responses and increases resistance to PRRSV infection in pigs.
format	article
author	Seo Byoung-Joo Lee Jee-Hoon Kang Ick-Jae Shabir Nadeem Khatun Amina Yang Myeon-Sik Park Chul Kim Bumseok Kim Won-Il
author_facet	Seo Byoung-Joo Lee Jee-Hoon Kang Ick-Jae Shabir Nadeem Khatun Amina Yang Myeon-Sik Park Chul Kim Bumseok Kim Won-Il
author_sort	Seo Byoung-Joo
title	Effects of high molecular weight poly-γ-glutamic acid on PIGS with porcine preproductive and respiratory syndrome virus (PRRSV) infection
title_short	Effects of high molecular weight poly-γ-glutamic acid on PIGS with porcine preproductive and respiratory syndrome virus (PRRSV) infection
title_full	Effects of high molecular weight poly-γ-glutamic acid on PIGS with porcine preproductive and respiratory syndrome virus (PRRSV) infection
title_fullStr	Effects of high molecular weight poly-γ-glutamic acid on PIGS with porcine preproductive and respiratory syndrome virus (PRRSV) infection
title_full_unstemmed	Effects of high molecular weight poly-γ-glutamic acid on PIGS with porcine preproductive and respiratory syndrome virus (PRRSV) infection
title_sort	effects of high molecular weight poly-γ-glutamic acid on pigs with porcine preproductive and respiratory syndrome virus (prrsv) infection
publisher	Sciendo
publishDate	2017
url	https://doaj.org/article/8ffc3d3af43a43ffaee573ff5cbf74ab
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Effects of high molecular weight poly-γ-glutamic acid on PIGS with porcine preproductive and respiratory syndrome virus (PRRSV) infection

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