In vitro evaluation of chemical decontamination of titanium discs

Abstract Peri-implant diseases are caused by bacterial biofilm colonizing implant surfaces. Prevention and management of peri-implant mucositis and peri-implantitis rely on effective biofilm removal. This study aimed to evaluate biofilm removal and cytocompatibility following chemo-mechanical surfac...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yuki Ichioka, Jan Derks, Gunnar Dahlén, Tord Berglundh, Lena Larsson
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8ffc756b5a2846818fc3067139a1328b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8ffc756b5a2846818fc3067139a1328b
record_format	dspace
spelling	oai:doaj.org-article:8ffc756b5a2846818fc3067139a1328b2021-11-28T12:19:38ZIn vitro evaluation of chemical decontamination of titanium discs10.1038/s41598-021-02220-32045-2322https://doaj.org/article/8ffc756b5a2846818fc3067139a1328b2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02220-3https://doaj.org/toc/2045-2322Abstract Peri-implant diseases are caused by bacterial biofilm colonizing implant surfaces. Prevention and management of peri-implant mucositis and peri-implantitis rely on effective biofilm removal. This study aimed to evaluate biofilm removal and cytocompatibility following chemo-mechanical surface decontamination of biofilm-coated titanium discs. Biofilm-coated (Streptococcus gordonii) discs, with either non-modified (smooth) or modified (rough) surfaces, were instrumented using a sterile gauze soaked in one out of four solutions: saline (NaCl), alkaline electrized water (AEW), citric acid (CA) or N-acetyl-l-cysteine (NAC). Non-contaminated, untreated titanium discs served as controls (C). Residual deposits (bacteria and gauze fibers) and cytocompatibility for osteoblast-like cells were evaluated using SEM and immunofluorescence. Cytotoxicity was assessed using WST-8 assay and immunofluorescence. All protocols were equally effective in removing bacteria from smooth surfaces, while AEW and CA were found to be superior at rough surfaces. AEW and NAC were superior in promoting cytocompatibility over NaCl. NAC and CA had a strong cytotoxic effect on osteoblast-like and fibroblast cells. In conclusion, AEW may be beneficial in the decontamination of implant surfaces, effectively removing bacterial biofilm and restoring cytocompatibility.Yuki IchiokaJan DerksGunnar DahlénTord BerglundhLena LarssonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuki Ichioka Jan Derks Gunnar Dahlén Tord Berglundh Lena Larsson In vitro evaluation of chemical decontamination of titanium discs
description	Abstract Peri-implant diseases are caused by bacterial biofilm colonizing implant surfaces. Prevention and management of peri-implant mucositis and peri-implantitis rely on effective biofilm removal. This study aimed to evaluate biofilm removal and cytocompatibility following chemo-mechanical surface decontamination of biofilm-coated titanium discs. Biofilm-coated (Streptococcus gordonii) discs, with either non-modified (smooth) or modified (rough) surfaces, were instrumented using a sterile gauze soaked in one out of four solutions: saline (NaCl), alkaline electrized water (AEW), citric acid (CA) or N-acetyl-l-cysteine (NAC). Non-contaminated, untreated titanium discs served as controls (C). Residual deposits (bacteria and gauze fibers) and cytocompatibility for osteoblast-like cells were evaluated using SEM and immunofluorescence. Cytotoxicity was assessed using WST-8 assay and immunofluorescence. All protocols were equally effective in removing bacteria from smooth surfaces, while AEW and CA were found to be superior at rough surfaces. AEW and NAC were superior in promoting cytocompatibility over NaCl. NAC and CA had a strong cytotoxic effect on osteoblast-like and fibroblast cells. In conclusion, AEW may be beneficial in the decontamination of implant surfaces, effectively removing bacterial biofilm and restoring cytocompatibility.
format	article
author	Yuki Ichioka Jan Derks Gunnar Dahlén Tord Berglundh Lena Larsson
author_facet	Yuki Ichioka Jan Derks Gunnar Dahlén Tord Berglundh Lena Larsson
author_sort	Yuki Ichioka
title	In vitro evaluation of chemical decontamination of titanium discs
title_short	In vitro evaluation of chemical decontamination of titanium discs
title_full	In vitro evaluation of chemical decontamination of titanium discs
title_fullStr	In vitro evaluation of chemical decontamination of titanium discs
title_full_unstemmed	In vitro evaluation of chemical decontamination of titanium discs
title_sort	in vitro evaluation of chemical decontamination of titanium discs
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8ffc756b5a2846818fc3067139a1328b
work_keys_str_mv	AT yukiichioka invitroevaluationofchemicaldecontaminationoftitaniumdiscs AT janderks invitroevaluationofchemicaldecontaminationoftitaniumdiscs AT gunnardahlen invitroevaluationofchemicaldecontaminationoftitaniumdiscs AT tordberglundh invitroevaluationofchemicaldecontaminationoftitaniumdiscs AT lenalarsson invitroevaluationofchemicaldecontaminationoftitaniumdiscs
_version_	1718408089515851776

In vitro evaluation of chemical decontamination of titanium discs

Ejemplares similares